An Investigation of Morpho-physiological Traits in Tobacco (Nicotiana tabacum L.) Somaclones

Nowadays, tobacco is considered as one of the important world industrial plants. In the present study, for a creation of somaclonal variation, calli were produced on MS medium supplemented by 2 mg/l of Naphthalene Acetic Acid (NAA) and then regenerated on MS medium supplemented with 1 mg/l Benzyl Amino Purine (BAP) and 0.1 mg/l of NAA. To determine correlations among traits and to fit multiple regression model to the green leaf yield, the produced somaclones and cv. Burley21 (as control) were investigated at the experimental field of Tirtash Research and Education Center. Different morpho-physiological traits were evaluated. Simple and canonical correlations were assessed and multiple regression was fitted on green leaf yield. Coefficients of canonical correlation indicated that there existed relationships between nicotine percentage and leaf yield, plant height and also between sugar percentage and plant height, leaf width as well as internode length. The most important characters entering multiple regression model were leaf length, stem diameter, nicotine percentage and plant height (R2=0.8). These traits can be employed, as some most important yield factors, by researchers in their future works.