Comparison of polymorphism in second intron of bovine Leptin gene in Iranian Taleshi and Holstein cattles using PCR-RFLP

There are main differences between two Iranian Taleshi and Holstein cattles in milk and meat production and reproduction traits. In this study, polymorphism in second intron of bovine Leptin gene was investigated in order to find molecular mechanisms of these differences in DNA levels. DNA extraction was carried out according to modified salting procedure from blood of one hundered cattles (70 Taleshi and 0 Holstein cattles) and then was amplified 4 bp from second intron of Leptin gene. PCR products were then digested with SauAI restriction enzyme for genotype frequency identification. Results revealed that AA, AB and BB genotype frequencies were 6.4,. 4 and 7.6 percent for Taleshi native cattles and 56.7, 4. and zero percent for Holstein cattles, respectively. Frequency of A and B alleles for Taleshi and Holstein cattles were also 77.,. 9 and 78.,. 7 percent respectively. According the result, polymorphism rate of Iranian cattles in Leptin gene was low and this might cause some breeding difficulties in future.