Isolation and Morphological and Molecular Identification of New Species of Cyanobacteria from Firoozkooh region (Tehran Province) Using Different Culture Media

Microalgae are cosmopolitan microorganisms and minerals are considered as limiting factors for growth of them. In the present study cyanobacteria were isolated and indentified and also the effect of different media on their growth was examined. Consequently, two new species of cyanobacteria belonged to the family of Oscillatoriaceae were isolated and reported for the first time from Tehran province. To this end, soil samples were collected from Tehran Province in summer 2008. Samples were cultured in BBM & N8 media to evaluate the effect of nutrients on the growth of microalgae. Isolation was carried out by several subcultures. Morphological indentification was done using systematics keys and partial sequencing of 16S rRNA was used for molecular identification. The results showed that two species were recorded from Tehran Province, Leptolyngbya sp. ISC 40 Gomont and Leptolyngbya sp. ISC 25 Gomont. The collected soil samples were analyzed physically and chemically. Results indicated that BBM medium was suitable for isolation and as indicated by infertility of cultured soils, two identified species are resistance to environmental factors.