Study of PAI-1 4G/5G polymorphism as a genetic risk factor for cardiovascular disease in the Iranian population

Two hundred and eight asymptomatic individuals from different origins of Iran were included in this study in order to assess the distribution of the PAI-1 4G/5G polymorphism responding for cardiovascular disease (CVD) in the general Iranian population using a novel technique reverse hybridization Strip Assay for the rapid and simultaneous detection. The test is based on multiplex PCR and hybridization to a teststrip presenting a parallel array of allele-specific oligonucleotide probes. The allele frequency of mutant PAI-1 4G/5G (0.45) is comparable to that of Europeans, but exceeded the much lower frequencies known from the most of Asia. Here we describe the distribution of mutant allele PAI-1 4G/5G in different ethnicities of Iranian population. Our data represent the only comprehensive study to date with respect to thrombophilic gene polymorphism in Iran.