In Vitro Optimization of Rooting in Two Genotype of Lentil (Lens culinaris Medik.)

Establishing of an efficient and repeatable regeneration protocol is one of the basic prerequirements for gene transformation and plant breeding. Lentil, because of rooting problems, is a recalcitrant legume in respect of whole regeneration. For this, we examined two methods in vitro and in vivo flowing in vitro method to induce root on regenerated shoots. In vitro-in vivo method was better than in vitro for rooting of regenerated shoots. The shoots regenerated from medium containing 2 mg/lit BAP produced higher frequency of rooting (70%) than medium containing 3, 4 mg/lit BAP that represents the inhibitory effect of cytokenins on root induction. IBA (10 mg /lit) as a overnight pretreatment, induced higher frequency of rooting than 3 days pretreatment while, NAA hormone with similar concentration as a 3 days pretreatment produced highest frequency of rooting (80%). Genotypes had no effect on percent, number and length of produced roots.