Polymorphism in the PEPCK-C Gene of Sistan and Baluchestan Native Chicken Breeds

PEPCK-C gene codes the phosphoenolpytuvate carboxykinase enzyme which has a key role in gloconeogenesis. There are two different types of PEPCK gene: mitochondrial PEPCK (PEPCK-M) and Cytosolic PEPCK (PEOCK-C). In these study 100 random samples from two native populations Khazak and Dashtiari in Sistan and Bluchestan region, namely, Khazak and Dashtiari were analyzed. DNA was extracted from feather pulps and PCR was carried out using primers F1R1, F2R2, F3R3 and F4R4 which amplifies 800 bp, 1000 bp, 1000 bp and 1080 bp fragments from the chicken PEPCK-C gene. The fragment covers from promoter to exon 3 of the PEPCK-C gene. PCR products were then digested by 2 restriction enzymes BstE Π and Aci I. Allelic as well as genotypic frequencies for two populations were determined and analyzed by Popgene software. Haplotype analysis in two population showed that there were three haplotypes B, C and D and their frequencies were 0., 0.84 and 0.02 for Khazak chickens and 0.02, 0.90 and 0.08 for Dashtiari chickens, respectively.