Comparison of Genetic and Morpho-physiological Distance via SDS-PAGE Marker in Some Rapeseed Genotypes

Oilseed crops such as rapeseed have the important role in the production ofenergy for humans. The most important part of plant breeding programsunderstanding of genetic diversity to classify population based on different markersfor selecting the suitable parents. In this research, genetic diversity of 16 genotypes ofrapeseed was evaluated using SDS-PAGE protein markers and morpho-physiologicaltraits in the field and laboratory conditions. Extraction of leaf proteins was performedbefore physiological maturity and then concentration of proteins was measured byBradford method. For separating and patterning of the extracted proteins, SDS-PAGEtechnique via poly acrylamide electrophoresis was used. After staining of proteins bycoomassie blue R-250 and scoring of the bands, cluster analysis was computed basedon Jaccard coefficient which that the genotypes classified into 2 separate groups.Results of cluster analysis based on agronomical traits shown that the studiedgenotypes classified into 5 groups. Correlation between morphological and moleculartraits was assessed using Mantel test and significant positive correlation was observedbetween them. Thus according to genetic distances between the genotypes, we can use cross between Milena and Dante for obtain the highest amount of heterosis in futurebreeding program and hybridization between Opera and Sahara based on morphophysiological markers according to similarity coefficient is justified.