The Investigation of silymarin effect on colon ulcer induced acetic acid in mice

The aim of this research is the investigation of anti- inflammatory effect of silymarin on the treatment of colon ulcer induced acetic acid in mice Balb/C spicies. In this study, 32 mice are divided into 4 groups (n=8).These groups include: control group with no colitis report, the Sham group with colitis report that are not treated and the groups with colitis report that received 40 and 80 mg/kg B.W. of silymarin orally.The groups with silymarin treatment initially received silymarin for 14 days before induction of the disease and then for 1 week after effected by disease. The drugs are fed to the mice per oral. To induce colitis, 1 ml of acetic acid (%4) is injected directly into the rectum. In the one week after induction of colitis, the animals sacrificed and the damages of the colon investigated with Murthy method. Also, the concentration of alkaline phosphatase enzyme measured. Also, tissue oedema verified. The results shown the concentration of alkaline phosphatase enzyme has been increased significantly (P<0.05) in colitis group in comparision with control group whear as the concentration of these enzyme has been decreased significantly (P<0.05) in treatment groups in comparision with colitis group. Also, acetic acid causes severe inflammation and damages of the colon. It is concluded treatment utilized silymarin can be considered as a suggestive way.