FISH study of chromosome 15 in a model effected with endometrial adenocarcinoma cancer

Main goal of this investigation were verification of gene's behavior which located on RNO15 with allelic imbalance during Endometrium cancer development. BDII/Han females were crossed to males from two other inbred rat strains known to have low incidence of EAC (BN/Han and SPRD-Cu3/Han). To generate F2 rats the F1 animals were intercrossed. In addition, backcross populations were generated by crossing male F1 rats to female BDII rats. In order to characterize these tumor-specific genetic aberrations in greater detail, we applied molecular cytogenetic methods (FISH, chromosome painting) to 18 rat endometrial adenocarcinoma (EAC) cell cultures. Analysis in the tumors by FISH analysis showed the presence of small regions of amplifications. These changes were cryptic so that they occurred inside cytogenetically normal-looking chromosomes. We detected rather specific chromosome aberrations (translocations, deletions, amplifications) often leading to copy number changes in small DNA segments (gains, losses).