Experimental studies of interactions between blastema tissue and three-demensional matrixes decellularized of New Zealand rabbit bladder in vitro

The histological events in experimental studies of interaction between blastema tissue and decellularized bladder of New Zealand rabbit invitro was the aim of this investigation. In order to decellularization of rabbit’s bladder, in physical way, the pieces of bladder tissue were frozen at -4°c for 24 hours, and kept in liquid nitrogen. In chemical Method, the bladder in 1% wt/vol solution of sodium dodecyl sulfate (SDS) for 24 hours to decellularization was kept. For blastema tissue preparation with the help of a special punch, New Zealand rabbit ear (pinna) was pierced, after 72 hours with the help of another puncher, a circular blastema from the edge of holes was separated and the decellularized samples were kept in to the middle of the blastema ring and then, transfered to the culture media in a sterile condition. The samples with hematoxylin & eosin, pick indigo and pickrofushin (van gaison) were stained. In this study, the studies of light microscopy showed that, colonial cells that connected together were perfectly migrated inside the scaffold on the day 15. On the same day, the blastema cells were differentiated to epithelial demensional matrix cells and bladder transitional epithelium created in the center of scaffold. Angiogenesis was observed in scaffold on the next days. It appears, the decellularized bladder of New-Zealand rabbit as a bio-scaffold is capable to provide a proper environment, induce blastema cells to migrate into the scaffold and create connections between blastema cells and differentiation.