Histochemical study of Verbascum speciocum extract's effects on the wound healing in rats

The aim of this essay was to assess the ointment effects of alcoholical extraction prepared from annual leaves of Verbascum speciocum grown in 'Bouein Zahra' area, on skin lesions made in Wistar rats. In this study, at in vivo condition, 36 adult male Wistar rats were divided in 3 groups: Control (no treatment), Sham (treated with petroleum jelly) and Experimental (treated with ointment verbascum extract in different doses). The operation day was considered as day zero. 10 days after treatment, up to the day of 14th, after the surgery on the killed rats, the samples were collected from wound part of each rat for histological analysis and also collagen special staining and average of epidermis thickness, and hair follicle & blood vessels diameter were done. There was an increase in hair follicles’ diameter in experimental groups with 1/3LD50 Ointment comparing with control groups also a significant increase was found in skin epidermis thickness in experimental groups with 1/4 LD50 versus control groups. Moreover a significant increase was observed in blood vessels’ diameter in experimental groups exposed to 1/3LD50 ointment to control. In this study, we realized that the external use of V. speciocum extract on skin lesion with high dosage has a cytotoxic effects and were shown necrosis in the treated tissues but the lower dosages of extraction have been shown better wound healing.