Assay of TGF-β and b-FGF on the Potential of Peripheral Blood-Borne Stem Cells and Bone Marrow-Derived Stem Cells In Wound Healing in a Murine Model

Effects of TGF-β and b-FGF on the Potential of Peripheral Blood-Borne Stem Cells and Bone Marrow-Derived Stem Cells In Wound Healing in a Murine Model Peripheral blood mesenchymal stem cells (PBMSCs) and bone marrow stem cells (BMSCs) cultured in media with transforming growth factor-beta (TGF-β) and basic fibroblast growth factor (b-FGF). Stem cells labeled with PKH26-GL, red fluorescent cell linker compound (Sigma, USA) and injected into tail vein of Models, Migration of cells histologically assayed, RT-PCR assay of collagen type I (COL1A1) transcripts. Our results show that the synergistic effects of transforming growth factor-beta (TGF-β-sigma) and basic fibroblast growth factor (b-FGF-sigma) lead to a significant increase in migration and recruitment of both PBMSCs and BMSCs to the wound site, with more potent effects on PBMSCs as compared with BMSCs. Reverse transcription polymerase chain reaction of collagen type I (COL1A1) transcripts (348 bp) confirmed that TGF-β and b-FGF activate collagen I (production in marrow stem cells at higher transcription levels), with more vigorous effects of TGF-β on PBMSCs as compared with the same condition on BMSCs. The growth factors have positive effect in wound healing potential of PBMSCs and BMSCs.