Study of the antioxidant effects of total and flavonoiods extracts of Cuscuta lehmanniana Bunge against on PC12 cells in vitro diabetic model

Central and peripheral neuropathies are the most common side effects of Diabetes Mellitus (DM). The exact mechanisms of diabetic neurotoxicity are still unknown. Recently oxidative stress is introduced as one of the factors for diabetics’ neuropathies. Antioxidants also used as therapeutic agents to reduce the side effects of diabetes. In the present research the antioxidant effects of total and flavonoid extracts of Cusseta lehmaniana Bunge on high glucose inducted Pc12 were studied. PC12 cell line treated with high glucose was used. Total and flavonoid extract of C. lehmaniana Bungs were prepared. PC12 cells treated with 6X fold high glucose concentration exposed to extracts. Antioxidant activity of extracts, cell viability and apoptosis were studied by DPPH, MTT, Annexin staining and Western Blotting respectively. The MTT result showed 50μg/ml of flavonoid extract and 100μg/ml of total C. lehmaniana Bungs extract increased cell viability after 3 hours pretreatment. DPPH assay demonstrated 50 μg/ml flavonoid extract can increased radical scavenging inhibits compare with ascorbic acid. Several assays were used for evaluated of anti apoptotic effect such as: Annexin, SubG1and Western blotting for expression of Bax protein. Based on our findings, it is concluded that both total and flavonoid extract of C. lehmaniana Bungs have the potential to protect PC12 cells against glucose neurotoxicity by reducing apoptosis via increased Bax expression protein.