A simple, fast and commercial method for trypsin purification from bovine pancreas

Trypsin is a serine protease found in the digestive system of many vertebrates، where it hydrolyses proteins. Trypsin has been used widely in various biotechnological processes. In this study trypsin was purified using two precipitation cut، 35% and 75% as saturation percent of Ammonium sulfate solutions، TCA precipitation (2. 5% W/V)، pH precipitation and finally affinity chromatography. This method could be used to obtain 2. 8 g purified trypsin with specific activity 155 U/mg and 26% recovery from 1kg bovine pancreas. Therefore، this method is a simple، fast and commercial method for scale-up of trypsin extraction from bovine pancreas.