Micropropagation of forest tree of Sorbus aucuparia.L by bud culture of mature plants

Sorbus aucuparia is an important slow growing forest tree with medicinal, industrial, and ornamental uses which is used for reforestation in high altitudes of mountainous areas. The species was endangered at northern forests of Iran. Therefore, micropropagation of adult trees through bud culture can help in conservation purposes of the species. For investigation of micropropagation of the species, apical and lateral buds from micro-cutting shoots of the adult trees were collected from Farim and Sangdeh, Sari Province. Pretreatments of sterilizing were cleaning and brushing of the buds with detergent and ethanol 70% solution and then dipping in 0.2 % fungicide of Tyram for 24 hours. Cleaning of buds with % 0.1 HgCl2 solution for 7 minutes for the samples of the end of fall season was the best treatment. The best medium for shoot regeneration was DKW medium with BA (0.5 mg/l), IBA (0.01 mg/l) and TDZ (0.05 mg/l). Lateral buds culture from shoot cutting was relatively successful in MS medium mixed with vermiculite. Pre-treatment for rooting of shoots was done in free hormone medium and rooting of shoots was done in modified MCM medium supplemented with 1 mg/l of IBA in dark treatment. The plantlets were successfully acclimated in green house