Investigation on casual and associated agents with bacterial canker stone fruit trees in Golestan Province

Bacterial canker and leaf spot are typical symptoms and of the most important diseases of stone fruit trees in world and in North your country. Significant management of plant disease is possible by identification and diagnosis plant pathogenic casual and associated agents. Various samples of canker and leaf spot tissues during 2007-2008 collected from different orchards of stone fruit trees of Golestan province and pathogenicity tests was demonstrated on peach young branches and leaf tissues by various bacterial isolates. Based on the morphological, nutritional, biochemical, physiological, total cellular protein pattern and PCR reaction with d21 and d22 primers Pseudomonas syringae pv. syringae (Pss), and Xanthomonas arboricola pv. pruni identified as casual agents of bacterial canker in this province. Pss was the major agent disease on the east regions such as Ali-Abad and Azad shahr but in west regions such as Kordkooy Xap was major agent. P.viridiflava isolated from gorgan areas only as associated agent with disease. This research is the first report on Xap, the casual agent of bacterial canker in stone fruit trees Golestan Province and also first report on P.viridiflava, as associated agent with bacterial canker stone fruit trees on the north regions Iran.