Identification of essential oil content and composition of Lippia citriodora seed

Lemon verbena (Lippia citriodora) is a medicinal plant, belonging to Verbenaceae family and essential oil has accumulated in its vegetative and reproductive parts. This study was to evaluate the quantity and quality of seed essential oil of lemon verbena (Lippia citriodora). After preparing healthy cuttings and treatment of them for IBA, root cuttings were transferred to the greenhouse for culture and care. After flowering and fruit ripening, seeds were collected and the seed essential oil was obtained by hydro-distillation method (30 grams of seed) and was dehydrated using dry sodium sulfate. The essential oil was analyzed by GC and GC/MS. Results showed oil output in operation was 31%. Twelve major compositions containing 80.12% of the total oil were identified. The main compounds of this essential oil were Geranial (34.1%), Neral (26.01%) and Limonene (7.04%). The amount of these compounds had increased so significant in comparison with these values in essential oil from vegetative parts.