Protein Changes of Ri T-DNA Carrier Transgenic Nicotiana tabacum L. Plant in Response to GA3 Treatment Comprising with Control

Plant growth regulators are able to change the physiological process. Aim of this study is studies of gibberellin (GA3) effects on soluble proteins and root and leaf protein banding profile of short stem Ri T-DNA carrier transgenic Nicotiana tabacum L. plant in comprising with control.

First, transformation of tobacco plant cv. Wisconsin was confirmed using PCR technique. Then transgenic and non transgenic plants were treated with 0,. 2. 4 mg/l GA3 in MS media under in vitro culture for 4 weeks. Soluble proteins as well as SDS PAGE of root and leaves from transgenic and non transgenic plants treated with GA3 were investigated.

Transformation in transgenic plant was confirmed using GUS primers. Assessment of roots and leaves protein of transgenic and non transgenic treated with different concentrations of GA3 revealed that exogenous GA3 had significant impact on increasing of total root protein of root and leaf in both transgenic and non transgenic plants. Also GA3 increased the number of protein bands in electrophoresis pattern.

It is believed that exogenous GA has an impact on root and leaf protein synthesis through transcription and translation processes and also inhibited the activity of protein degradation enzymes.