Acute Treadmill Exercise Increases Heat Shock Protein 72 and Total Antioxidant Capacity Level in The Streptozotocin-Induced Diabetic Rat Hippocampus

The purpose of this study was to investigate the effects of acute treadmill exercise on Hsp72 and TAC in the hippocampus of diabetic rats. Forty male rats, weight 165±1 g, were assigned randomly into 4 groups: Diabetic control group, and 3 diabetic+acute training groups, including groups 1,2 and 3, the animals of which were sacrificed 30 minutes, 4 hours and 24 hours postexercise respectively. Diabetes was induced by injecting streptozotocin (STZ; 0/5 mg/kg dissolved in 0.1 M citrate buffer, pH 4.5) into the abdominal cavity. Rats were subjected to treadmill exercise, (treadmill speed 10 m/min, gradually increased to 18 m/min, grade 0%, duration exercise 60 min). Data were analysed with one-way Anova and tukey post hoc, P≤0/05. Acute exercise training increased the hippocampal HSP72 in all groups, but a significant increase took place only in the group 2, compared with the control group (1/82 mg/ml, P=0/043). TAC level increased significantly in group 1 (P=0.036) compared with the controls. but increase in group 2 and 3 was not significant. These results show that acute treadmill exercise improved HSP72 and TAC levels in the hippocampus of diabetic subjects and can have beneficial effects, and can hence be recommended as a therapeutic strategy for diabetes.