Study on Association of Peroxisome Proliferator-Activated Receptor α with C-Reactive Protein, and Additional Gene- Gene Interaction in Chinese Han

The aim was to examine the association between 6 single nucleotide polymorphisms (SNPs) of peroxisome proliferator-activated receptors α (PPAR α) poly morphisms and C-reactive protein (CRP) level, as well as additional gene-gene interaction among the 6 SNPs. A total of 1260 subjects (583 men, 677 women), with a mean age of 41.3 ± 14.6 years old, were selected. Six SNPs of PPAR α were selected for genotyping in the study including: rs135539, rs 135551 and rs135549, rs1800206, rs1800243,and rs4253623. Linear regression analysis was performed to verify the polymorphism association between SNP with CRP levels. Generalized MDR (GMDR) was employed to analysis the interaction among six SNPs. Linear regression results indicated a significant negative correlation between mutation of rs1800206 and CRP level. The carriers of the V allele (LV + VV) of rs1800206 were associated with a significant decreased level of CRP (regression coefficients was -0.533, standard error was 0.148 (P < 0.001)). However, the other 5 SNPs in PPAR α were not significantly associated with CRP level before or after covariate adjustment. GMDR model indicated that there was a significant two-locus model (P = 0.0107) involving rs1800206 and rs135539, indicating a potential gene–gene interaction between rs1800206 and rs135539. Overall, the two- locus models had a cross-validation consistency of 10 of 10, respectively, and had the testing accuracy of 55.9%, respectively. Our results support an important association between rs1800206 minor allele (V) of PPAR α and lower CRP level. The interaction analysis showed a combined effect between rs1800206 and rs135539 on the lower CRP level.