Selective Spectrophotometric Determination of Metformin Hydrochloride in Pharmaceuticals and Urine Using Two Nitrophenols as Chromogenic Agents
Author(s):
Abstract:
Metformin hydrochloride (MFH) is an oral anti-diabetic drug of biguanide class. Two selective spectrophotometric methods were presented for the determination of MFH in pharmaceuticals. The methods were based on the measurement of yellow-coloured charge-transfer complexes formed between MFH and two poly-nitrophenols, namely, 2,4-dinitrophenol (DNP method, at 405 nm) and picric acid (PA method; at 410 nm) in dichloromethane medium. The variables which affect the complex formation were studied and optimized. Beers law was obeyed over the concentration ranges: 2.4-48.0 and 3.2-64.0 µg ml-1 with molar absorptivity values of 3.24 × 103 and 2.30 × 103 l mol-1 cm-1, with DNP method and PA method, respectively. The limits of detection (LOD) and quantification (LOQ) were calculated to be 0.13 and 0.40 µg ml-1 for DNP method and 0.19 and 0.59 µg ml-1 for PA method. Methods were validated for accuracy, precision, robustness, ruggedness and selectivity. The proposed methods were applied to the determination of MFH in tablets. The accuracy and precision of the methods were found excellent. Accuracy of the methods was ascertained by recovery test via standard-addition procedure. The methods were applied to spiked human urine sample without detectable interference from endogenous substances.
Keywords:
Language:
English
Published:
Analytical and Bioanalytical Chemistry Research, Volume:4 Issue: 1, Winter - Spring 2017
Pages:
41 to 51
magiran.com/p1645568
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!