Simultaneous Determination of Pyridine-2-aldoxime Methochloride and Dopamine at Poly(3,4-ethylenedioxythiophene) Modified Glassy Carbon Electrode

A simple and sensitive voltammetric method was developed for simultaneous determination of pyridine-2-aldoxime (PAM-2) and the neurotransmitter dopamine (DA) at poly(3,4-ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode. The electrochemical behavior of DA and PAM-2 were investigated using cyclic voltammetry. PAM-2 was irreversibly oxidized at 650 mV while DA was reversibly oxidized and reduced at about 185 mV and 147 mV, respectively. The observed significant oxidation peak potential difference between PAM-2 and DA allowed the simultaneous determination of both species using square wave adsorptive stripping voltammetry. Under optimized conditions, the voltammetric responses gave linear ranges of 3.0×10−6–1.5×10-4 M and 1.0×10−7–1.0×10−4 M with detection limits of 1.9×10−7 M and 3.1×10−8 M for PAM-2 and DA, respectively. The determination of PAM-2 and DA in human blood serum samples was successfully carried out with a very good recovery result 95.17% and 101.6%, respectively.