Pre-treatment with rapamycin protects hematopoiesis against radiation injury

Protection of hematopoietic system has become a primary goal in the development of novel medical countermeasures against ionization radiation and radiotherapy. This study was to explore the role of rapamycin in normal tissues against radiation.
Mice were pretreated with rapamycin by i.p. every other day for five times before 5 Gy or 8.5 Gy γ-ray whole body irradiation. Blood cell counts, HE staining of bone marrow and liver, bone marrow transplantation, CFU of spleen were used to measure the damage of hematopoiesis and extramedullary hemopoietic organs. Regular karyotype analysis and expression of γ-H2AX (by flow cytometry and western blot) were used to measure DNA damage. Rad 50 and DNA Lig 4 expression by western blot were to see the DNA repair ability.
The decrease of red blood cells and platelet induced by radiation were alleviated by pretreatment with rapamycin (d 7,15, p Rapamycin pretreatment mitigates hematopoietic system from radiation injury in both bone marrow and extramedullary hematopoietic organs by improving genomic stability and increasing survival of hematopoietic stem and progenitor cells (HSPCs).