Cloning and Expression of HP0242 Hypothetical Gene as a DNA Vaccine Candidate for Helicobacter pylori and Study of Its Immunoreactivity

The HP0242 plays an important role in physiology of H. pylori in acidic condition.
The aim of this study was cloning and expression of immunogenicity of HP0242 gene of H. pylori.
The whole sequence of the hypothetical gene was amplified with specific primers. The DNA sequence (716 bp) was cloned into E. coli TOP10F by utilizing PEGFP-C1 and PTZ vectors. The protein product in CHO cells was elucidated with the SDS-PAGE technique. Western blot analysis showed that HP0242 expressed by recombinant E. coli had favorable immune-reactivity. Western blot was conducted to assess the immune-reactivity.
Presence of hypothetical gene in the expression PEGFP-C1 vector was confirmed with digestion by sacII and smaI. Finally, the expression of the hypothetical protein in CHO cells was confirmed by SDS-PAGE and thus, can be considered as a DNA vaccine candidate for H. pylori. Western blot analysis showed that HP0242 expressed by recombinant E. coli had a favorable immune-reactivity.
The expressed HP0242 gene showed the possibility that it can be used as a candidate DNA vaccine for H. pylori in the future research.