The Effects of Combination of Coix Seed Extract and Cisplatin on TAM and Expression of HIF-1αin vivo in Lewis Lung Carcinoma

We investigated the combined effects of Kanglaite (KLT) and cisplatin on tumor associated macrophage (TAM) and expression of hypoxia inducible factor-1 alpha (HIF-1α) in Kunming mice with Lewis lung carcinoma.
Kunming mice with Lewis lung carcinoma were randomly divided into four groups: the control (NS) group, KLT group, cisplatin (DDP) group and DDP⢴ group in Hebei People's Hospital, Hebei China from 2016 to 2017. Tumors were harvested 14 days after corresponding interventions.
The percentage of TAM was determined by flow cytometry and HIF-1α mRNA was detected by realtime-PCR. Tumor weight of mice in KLT group, DDP group and DDP⢴ group was significantly lower than that of NS group (P KLT led to pronounced antitumor activity in mice with Lewis lung carcinoma. It enhances the chemotherapeutic effect and improves immunity function when combined with cisplatin, which can be accomplished by decreasing the TAM levels and improving hypoxia status.