Evaluation of Phytochemistry and Some Biological Activities of Aerial Parts and Seed of Scrophularia Umbrosa Dumort

Scrophularia umbrosa is a plant used as a traditional herb.
This study was designed to investigate the antimalarial activity and free radical scavenging of methanol, DCM and n-Hexane extract of aerial parts and seed, as well as total phenol, total flavonoid contents, and phytochemical identification.
GC-MS technique were used for identification of the chemical compositions of the DCM and n-Hexane extract as well as HPLC and NMR techniques for methanolic extract. The hemozoin formation has been suggested as a desirable drug target to antimalaria sieve scheme. In the present study, we used the spectrophotometric assay to screen the potency of plant extracts. The DPPH method has been used for antioxidant activity screening of the extracts and fractions. AlCl3 and Folin -Ciocalteu reagents, respectively, were used for total flavonoid and total phenol contents of samples.
Our results showed that n-hexane and the MeOH extracts of the aerial parts, and seed did not display any impressive prevention of heme biocrystallization confidants, however, the DCM extract of aerial part, in the comparison with the blank, showed slightly antimalarial effects. Furthermore, fractions (20%, 40%, and 60%) of MeOH extracts of aerial part and seed of S. umbrosa had moderate to strong antioxidant activity and it is possible to conclude that there is a positive relation between phenolic and flavonoid compositions as well as antioxidant activity. GC-MS data showed that volatile portions of DCM and n-Hexane extracts of various organs from S. umbrosa are containing few identifiable compounds. In addition, Aucubin and Luteolin-7-O-rutinoside were also recognized from MeOH extracts.
Based on the results, the fractions of extracts could be evaluated by 1HNMR for predicting of groups of natural compounds as well as interfacing chemical and biological assessments.