Characterization of beta tubulin DNA sequences within the Candida parapsilosis complex
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and
Results and
Purpose
Candida parapsilosis is a common cause of candidemia in children and onco-hematologic patients as well as in patients with septic arthritis, peritonitis, vaginitis, and nail and skin infections. Here, we evaluated intra- and inter-species beta tubulin DNA sequence variation within the C. parapsilosis complex with a view to establishing its utility in the identification and phylogenetic analysis of the species.Materials And Methods
The novel primers successfully amplified the target for all 23 strains included in the study.Results and
Conclusion
Multiple alignment of 623629 bp-long nucleotide (nt) sequences reflecting the beta tubulin gene indicated an inter-species divergence ranging from 0 to 68 nt in C. parapsilosis, C. orthopsilosis and C. metapsilosis with a mean similarity of 84.7% among the species. Meanwhile, intra-species differences of 020 and 06 nt were found within the strains of C. parapsilosis and C. orthopsilosis, respectively. The phylogenetic tree topology was characterized by a clade made up by C. parapsilosis and C. orthopsilosis, while C. metapsilosis formed a related but separate lineage. Our data provide the basis for further discoveries of the relationship between the species belonging to the C. parapsilosis complex and the value of beta tubulin DNA sequence data in the identification and taxonomy of C. parapsilosis and other pathogenic yeasts.Keywords:
Language:
English
Published:
Current Medical Mycology, Volume:4 Issue: 1, Mar 2018
Pages:
24 to 29
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