Designing a novel ELISA method to increase sensitivity and specificity of Helicobacter pylori whole cell antigen detection
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Aim
In this research, we designed a direct Enzyme Linked Immunoassay method to detect Helicobacter pylori antigens in stoolspecimens.
Background
Helicobacter pylori infection as the worldwide problem is related to many gastrointestinal disorders such as gastritis,gastric cancer, non-ulcer disease, peptic ulcer disease and duodenal ulcer.
Methods
We produced and purified recombinant CagA and NapA antigens in Escherichia coli and extracted their antibodies from apanel of positive sera specimens. We designed a novel enzyme linked immunoassay direct method in combination with the whole cell
for the qualitative and quantitative detection of Helicobacter pylori antigens in human stool. Assay performance was evaluated by
histopathology staining and urease activity.
Results
The sensitivity and specificity of assay was determined as 91.7 [95% confidence interval: 89.3–95.6%] and 93.1% [95% CI:91.2–96.4%], respectively. Novel ELISA exhibits enhanced sensitivity and specificity of Helicobacter pylori detection in comparisonwith another commercially available kit.
Conclusion
Combination of the recombinant antigens and whole cell of Helicobacter pylori in immunoassay designing is a newapproach about early diagnosis, treatment and fallowing up of the Helicobacter pylori infected patients, especially in peptic cancer
cases
Keywords:
Language:
English
Published:
Gastroenterology and Hepatology From Bed to Bench Journal, Volume:11 Issue: 4, Autumn 2018
Pages:
333 to 342
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