CRISPR-Cas9 Mediated Capsule Gene Silencing in Escherichia coli

Genomic engineering of Escherichia coli is applied to design and produce recombinant proteins as the new drugs. The aim of this study was to CRISPR-Cas9 mediated capsule gene silencing in E. coli. We suppressed genes involved in capsule expression of E.coli by CRISPR cas9 process. The constructed E.coli was confirmed by microscopic smear, transmission electron microscopy and T7 phage influence assay. The results were shown that the inhibition of capsule production was carried out successfully and there was not any capsule layer around the bacteria. E. coli without any capsule around may proper for replacement of it with other molecules in future.