Prediction of Rejection in Ranal Transplantation by immune parameters

16 non-diabetic kidney transplant candidates (4 females and 12 males، age = 20-65 yr، first time transplant) were selected. The transplanted patients were divided into two groups based on the rejection during 3 weeks post transplant: group I (n = 9) without rejection and group II (n = 7) with a rejection episode. Immune parameters including lymphocytes subpopulations (by flowcytometry) and immunoglobulin classes (IgM، IgG، IgA and IgE by nephlometric assay) before and 45 days after transplantation were determined. The results of this investigation showed that the level of immunoglobulin IgG، IgM، IgA and IgE decreased post transplantation due to immunosuppressive drugs. CD3، CD4، CD8 T cells count، CD56 NK cells count and CD20 B cells count pre- and post-transplantation did not show any significant differences. The amount of IgE (220 vs. 462 IU/ml)، CD3 (62% vs. 69. 7%) and CD4 (35% vs. 41. 3%) cells increased in group II during rejection episode pre-transplantation. In addition، IgA increased pretransplantation in group I those without rejection episode in comparison with group II with a rejection episode. Forty five days post transplantation IgA (209 vs. 152 mg/dl)، IgG (1009 vs. 703 mg/dl) and CD20 (15% vs. 10%) increased in group I patients. It is suggestive that pre-transplantation increases IgE، CD3 and CD4 are predictive of acute rejection.