Detection of Brucella by Peripheral Blood PCR and Comparison ith Culture and Serological Methods in Suspected Cases

Author(s):

B. Kazemi , Sa Yousefi Namin , M. Dowlatshahi , M. Bandepour , F. Kafilzadeh , L. Gachkar , Fmahmoudinejad , A. Samarghandi , M. Mardani

Message:
Abstract:
Background
Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi tional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR method is recommended.
Methods
One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form Ker manshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture and PCR methods.
Results
Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 sam ples were positive by serological methods.
Conclusion
PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases.
Language:
English
Published:
Iranian Journal of Public Health, Volume:37 Issue: 4, Winter 2008
Page:
96
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