Studies of Human HbAA Erythrocyte Osmotic Fragility Index of Non-Malarious Blood in the Presence of Five Anti-malarial Drugs

The capacity of human HbAA erythrocytes of non-malarious blood to withstand osmotic stress in the presence of five antimalarial drugs، Chloroquine phosphate، Quinine، FansidarTM، CoartemTM and HalfanTM was studied in vitro. Aqueous solutions of four increasing concentrations of the drugs used in this investigation were in the order: 0. 2%، 0. 4%، 0. 6% and 0. 8% (w/v). Spectrophotometric method was employed to ascertain the osmotic fragility index of the erythrocytes. The mean corpuscular fragility (MCF) index (X+S. D) of the control sample was 0. 351+0. 06 g/100ml. The MCF values of the control and test samples were statistically compared (p value = 0. 05). At the drug concentration of 0. 8g، MCF values (g) of 0. 401±0. 005، 0. 391±0. 003، 0. 364±0. 02، 0. 344±0. 02 and 0. 338±0. 04 were obtained for halfan، coartem، quinine، chloroquine phosphate and fansidar، respectively. An overview of the results showed that in a concentration dependent manner، Choroquine phosphate and FansidarTM exhibited a diminishing capacity to stabilize red blood cell membrane while HalfanTM CoartemTM and Quinine elicited an increasing propensity to disrupt erythrocyte membrane integrity