Preliminary Identification and Typing of Pathogenic and Toxigenic Fusarium Species Using Restriction Digestion of ITS1-5.8S rDNA-ITS2 Region
Message:
Abstract:
Background
Fusarium species are capable of causing a wide range of crop plants infections as well as uncommon hu­man infections. Many species of the genus produce mycotoxins, which are responsible for acute or chronic diseases in ani­mals and humans. Identification of Fusaria to the species level is necessary for biological, epidemiological, pathologi­cal, and toxicological purposes. In this study, we undertook a computer-based analysis of ITS1-5.8SrDNA-ITS2 in 192 GenBank sequences from 36 Fusarium species to achieve data for establishing a molecular method for spe­cie-specific identification.
Methods
Sequence data and 610 restriction enzymes were analyzed for choosing RFLP profiles, and subsequently de­signed and validated a PCR-restriction enzyme system for identification and typing of species. DNA extracted from 32 refer­ence strains of 16 species were amplified using ITS1 and ITS4 universal primers followed by sequencing and restric­tion enzyme digestion of PCR products.
Results
The following 3 restriction enzymes TasI, ItaI and CfoI provide the best discriminatory power. Using ITS1 and ITS4 primers a product of approximately 550bp was observed for all Fusarium strains, as expected regarding the se­quence analyses. After RFLP of the PCR products, some species were definitely identified by the method and some strains had different patterns in same species.
Conclusion
Our profile has potential not only for identification of species, but also for genotyping of strains. On the other hand, some Fusarium species were 100% identical in their ITS-5.8SrDNA-ITS2 sequences, therefore differentia­tion of these species is impossible regarding this target alone. ITS-PCR-RFLP method might be useful for preliminary differ­entiation and typing of most common Fusarium species.
Language:
English
Published:
Iranian Journal of Public Health, Volume:39 Issue: 4, Winter 2010
Pages:
35 to 44
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