International Journal of Advanced Biological and Biomedical Research
Volume:11 Issue: 2, Spring 2023

The present study examines the effects of siRNA on the expression level of Snail1 and miR-143 genes in metastatic breast cancer cells during mastectomy and the results of this study are in the field of etiology and cellular and molecular foundations. The synthesis of cDNA strand was done with the help of hexamer primer, which binds non-specifically at different points of the RNA strand, and as a result, all RNA strands are used as synthesis templates. For this purpose, Total RNA was first extracted from the cell culture medium for cDNA synthesis. The results of knockdown evaluation of Snail1 gene by specific siRNA showed that the knockdown of this gene in metastatic cancer cells increases the relative expression of miR-143 in the effective dose and time compared to untreated cells. Transfection of breast cancer cells by specific siRNA by reducing the relative level of snail1 gene expression and increasing the relative level of miR-143 expression can successfully reduce proliferation and invasion. become breast cancer cells and this can play an important role in the use of cellular and molecular methods in the treatment of breast cancer.

Spodoptera littoralis Boisad. (Lepidoptera: Noctuidae) is a serious pest that harms crops all over the world. This pest is frequently treated with insecticides, but in many countries, its resistance impairs field control. The aim of the current study is to determine the resistance evolution to different insecticides in S. littoralis for the possibility of detecting susceptibility shifts that lead to poor control. Leaf-dip method was used to conduct the pesticides bioassay to determine the resistance stability to several conventional and new insecticides under laboratory settings in field population of S. littoralis. Emamectin benzoate (2.00 ppm) was shown to be the most toxic compound based on LC50 values in the first generation, followed by spinosad (8.62 ppm), chlorantraniliprole (79.44 ppm), lambda-cyhalothrin (270.44 ppm), and profenofos (313.96 ppm). After twelve generations, the LC50 values for emamectin benzoate, spinosad, chlorantraniliprole, lambda-cyhalothrin, and profenofos decreased 4.88-, 9.91-, 9.42-, 16.52-, and 16.85- times, respectively, in field-collected of S. littoralis. The estimated drop in resistance was 17.08, 12.11, 11.96, 10.41, and 9.29 for emamectin benzoate, chlorantraniliprole, spinosad, profenofos, and lambda-cyhalothrin, respectively. According to the findings, insecticides with novel modes of action had higher reversal rates and less stable resistance to spinosad and chlorantraniliprole.

It should be noted that considering that the present study seeks to understand the transition from cancer to partial recovery, survivors who have recovered their emotional balance and relative health after mastectomy were selected as the studied group. Based on this, in the current study, the survivor is someone who has completed his active treatments and at least 5 years have passed since his partial recovery. Data saturation was reached by conducting 19 interviews. Lincoln and Goba criteria were used to ensure the validity and strength of the research. For this purpose, the credibility of the research was ensured by using strategies such as researcher's self-review, review by members and experts. In this study, seven explanatory codes have been described with cognitive changes in the phenomenon of survival and transition from illness to recovery among the studied survivors. In this transition, organizing the survivors' cognitive experiences reveals two different cognitive systems including the perception of the stage before and after cancer. Swimming with the experience of the survivors shows that sex, age and class discriminations have been internalized with the family's educational strategies. The reproduction of these discriminations with the rules governing the objective positions as well as cognitive patterns affects the health of people. Based on this, it is suggested that policymakers and researchers pay attention to the solutions to eliminate poverty and discrimination by focusing on recognizing and reconstructing the cognitive pattern of people, the objective context of its formation.

Low-level laser therapy (LLLT), is a clinically well-established tool for wound healing. In vitro studies have shown that LLLT has abiostimulatory effect on mesenchymal lineages and non-mesenchymal lineages of Adipose-derived stem cells (ADSCs) differentiation. The aim of this study was to evaluate the effects of LLLT at different energy intensities on differentiation of (ADSCs) to keratinocyte. Adipose tissue were successfully obtained from 45-year-old woman by Lipoaspirates and adipose-derived mesenchymal stem cells (ADSCs) were isolated. ADSCs were divided into a control group and three low-level laser treated groups irradiated with 0.5 J/cm2, 1 J/cm2 and 2 J/cm2 laser doses using He-Ne laser with 632nm wave length in 5 times. All groups cultured with keratinocytes media for 10-days. Cell proliferation was eveluated using 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. ADSCs developed a polygonal cobblestone shape characteristic of human keratinocytes. Specific keratinocyte marker 5 (KRT5) were analyzed by real-time PCR. Data were analyzed using one-way AVOVA and Tukey-Kramer hoc test by SPSS software version 16. Results of this study demonstrated LLLT could significantly affect proliferation of ADSCs (P<0.05) and promote ADSCs differentiation to keratinocytes by increasing expression of KRT5 at of 1 J/cm2 and 2 J/cm2 laser doses (P<0.01). LLLT at density of 0.5 J/cm2 could not promote ADSCs differentiation significantly. Considering these findings, LLLT could improve current in vitro methods of differentiating ADSCs to keratinocyte prior to transplantation. Thereby LLLT can use clinically as promoting skin wound healing.

Withing a short period of time up to six months after brain damage, the brain can be reconstructed and plasticity can be accelerated. Therefore, there is a possibility of recovering a collection of motions by rehabilitation within this time period. The present article investigated the process of neuromotor rehabilitation in patients with brain lesions, plus developed a skill in healthy people by analyzing the neuroplasticity and studied the changes of muscle synergy.

The present Sysyematic review researched the combination of terms such as rehabilitation(Mesh), neurorehabilitation(Mesh), brain stroke(Mesh), brain lesion(Mesh), skill (Mesh)with no time limit on scientific databases including Science Direct, Springer, Google Scholar, Pubmed, Scopus, SID, IranDoc, and Web of Science and the purpose of the research was specified on the basis of the articles found.

Among the researched articles, 32 related articles were reviewed, the most important of which were investigated and analyzed included rehabilitation in stroke, rehabilitation, and spasticity, rehabilitation recovery mechanisms in spasticity after a stroke, comparison between the effects of rehabilitation after a stroke and healthy people, required after stroke training for patients, the effectiveness of repetition in the rehabilitation exercises after stroke.

The process of neuromotor rehabilitation of patients with brain lesions and the development of skills in them compared to healthy individuals by analyzing the degree of plasticity is an effective way to strengthen muscles and can be effective; However further investigation is needed.

considering that intravenous acetaminophen is currently usually injected as a bolus, in such a way that it should be injected for 20 minutes and 4 times a day to patients undergoing surgery, and considering the lack of nurses and the fact that practically This work is not cost-effective in terms of time and money, and there was no research in the field of intravenous drip injection within 24 hours. Therefore, this study was conducted with the aim of investigating the effectiveness of intravenous acetaminophen injection during the first 24 hours after cesarean section in a systematic review.

In this review article, all databases including Google Scholar, Scopus, Web of Science, PubMed, SID, MagIran, and the Cochrane Library were searched and reviewed by both authors of this article based on PRIZMA guidelines without time and language limitations. The keywords included Pain, Pain relief, Postoperative Pain, Pain Decrease, Cesarean Section, Acetaminophen, and Paracetamol.

Ten studies were included in this study. Examining the results indicated that intravenous injection of paracetamol leads to a decrease in pain intensity during the first 24 hours after elective cesarean section.

Intravenous injection of acetaminophen for pain control after cesarean section by PCA pump, in addition to proper pain control, maintains the hemodynamic indicators of patients well and does not have an adverse effect on the time of walking after the operation.