ابراهیم غلامعلی پور علمداری

Intercropping, the agricultural practice of cultivating two or more crops in the same space at the same time, is an old and commonly used cropping practice which aims to match efficiently crop demands to the available growth resources. The practice of growing two or more crops simultaneously in the same field is called intercropping and it is a common feature in traditional farming of small landholders. It provides farmers with a variety of returns from land and labour, often increases the efficiency with which scarce resources are used and reduces the failure risk of a single crop that is susceptible to environmental and economic fluctuation. Intercropping of chickpea with linseed reduced the chickpea yield by 60.3%, although linseed occupied only 33% of the total area. The loss of chickpea yield was compensated by the additional yield of linseed, and thus the system productivity of chickpea + linseed intercropping was increased by 43.4% compared with sole chickpea. Of this increase in system productivity, 65.3 and 34.7% were contributed by higher seed yield and higher minimum prices of linseed, respectively as compared to chickpea. The objectives of the present study were to study the competition indices of chickpea and quinoa intercropping such as land equivalent ratio, relative crowding coefficient, aggressivity, chickpea equivalent yield and effects of nitrogen and different intercropping arrangements on yield of sole chickpea, sole quinoa and intercropping yield of two plants. The objectives of the present study was to determine the best appropriate treatment of sole cropped, replacement and additive series of chickpea and quinoa in terms of yield and investigation of competition indices in intercropping replacement and additive chickpea and quinoa.

In order to study the effect of planting pattern and nitrogen application on yield and competitive indicators of chickpea and quinoa, a factorial experiment based on randomized complete block design (RCBD) was conducted with three replications at Gonbad Kavous University farm during the 2020-2021 growing season. Nitrogen factor was included three levels of non-application and application of 25 and 50 kg N.ha-1 and the treatments of intercropping were included 9 levels of sole cropped of chickpea and quinoa, 67% chickpea + 33% quinoa, 50% chickpea + 50% quinoa, 33% chickpea + 67% quinoa, 100% chickpea + 33% quinoa, 100% chickpea + 50% quinoa, 100% chickpea + 67% quinoa and 100% chickpea + 100% quinoa. For analysis variance of data software of SAS Ver. 9.4 was used and treatment mean differences were separated by the least significant difference (LSD) test at the 0.05 probability level.

Results showed that the effects of planting pattern and nitrogen consumption on yield were significant at confidence level of 1%. The maximum total yield belonged to the treatment sole cropped of chickpea with 3529 kg.ha-1, which was not significantly different from the 67% chickpea + 33% quinoa treatment. With increase of nitrogen fertilizer consumption, total yield was increased. The lowest chickpea crop equivalent yield related to the treatment sole cropped of chickpea and mixed crop was 33% increase of quinoa to chickpea crop with 3529 and 3551 kg.ha-1, respectively, and the highest was related to sole cropped of quinoa with 8876 kg.ha-1. Land equivalent ratio was higher than one in all the additive mixed cropping treatments and alternative mixed cropping treatments. The maximum proportion of land belonging to the treatment of 100% chickpeas + 67% quinoa was 1.10. The relative coefficient of total density in all treatments was more than one, which indicates the desirability of mixed cultivation of chickpea and quinoa. The examination of the aggressivity index showed that quinoa was dominant in the mixed cropping treatments, increasing and replacing the plant. As a result, the dominance index of quinoa was positive despite the favorable yield of both plants.

Of the two plants of chickpea and quinoa, quinoa plant produced more yield than chickpea plant. The maximum total yield belonged to the treatment sole cropped of chickpea with 3529 kg.ha-1, which was not significantly different from the 67% chickpea + 33% quinoa treatment. With increasing of nitrogen fertilizer consumption, yield was increased. According to the results, it seems that it is not possible to increase the density of two plants in mixed cultivation to obtain the maximum yield.

In order to investigate the effect of nitrogen fertilizer and foliar spraying of humic acid and Vita free on phenological stages, remobilization indices and yield of sunflower in the second experimental planting in a factorial manner based on a randomized complete block design with with three replications in summer 2018 at Gonbad Kavous University farm research. Cultivar factor was included Haysan 25 and Oscar and combination of fertilizer factor was included no fertilizer application, 100 kg N/ha, 50 kg N/ha, 50 kg N/ha + 1.5 kg humic acid/ha, 50 kg N/ha + 0.5 kg Vita free/ha and 50 kg N/ha + 1.5 kg humic acid + 0.5 kg Vita free/ha. The results showed that the effect of cultivar and fertilizer on most of the measured traits was significant. The results showed that cultivar and fertilizer were significant on most of the studied traits. The maximum days from planting to 50% Flowering, the dry matter of vegetative organs in the flowering stage, the remobilization, current photosynthesis and seed yield were obtained from Oscar cultivar. The maximum remobilization and remobilization efficiency were obtained in the treatment of no fertilizer use. Days from planting to 50% flowering, days from planting to 50% maturity, current photosynthesis and seed yield in 100 kg N/ha treatment and 50 kg N/ha + 1.5 kg humic acid/ha + 0.5 kg Vitafree/h was higher than other fertilizer treatments. Based on results, in order to achieve the maximum seed yield of sunflower and to prevent excessive use of chemical fertilizers, the combined use of Nitrogen fertilizer, humic acid and vita free is necessary.

To study the allelopathic effect of decomposed fresh aerial parts of Rapistrum rugosum on growth, physiological and biochemical traits of Hibiscus sabdariffa, a pot experiment was conducted as a completely randomized design with three replications under greenhouse condition at the Gonbad Kavous University. Treatments were different amounts of R. rugosum residues (0, 10, 20, 40, 80, and 160 g kg-1 soil). The results showed that increasing the amount of R. rugosum residues significantly reduced the growth traits of H. sabdariffa. The lowest amount of stem and root length, fresh and dry weight of the plant was observed in 160 g kg-1 of R. rugosum residues. The content of chlorophyll b and carotenoids, as well as malondialdehyde, decreased with increasing the amount of R. rugosum. However, the trend of changes in the content of catalase enzyme, soluble sugars content, proline, total phenol, and flavonoids increased with increasing the amount of R. rugosum residues. Consequently, our findings showed that with the increasing in the amount of R. rugosum residues, all the studied traits of H. sabdariffa decreased significantly. Therefore, it can be recommended to use H. sabdariffa weed as a natural herbicide in sustainable agriculture especially in medicinal plant production.

Chemical compounds in plants include primary or secondary compounds. The allelopathic compounds are mainly of the secondary type, which are released by ways such as leaching, decomposition of plant residues, volatilization, and root exudation. Plants with allelopathic properties have a negative or even positive effect on the germination and growth of other plants by releasing substances in their surroundings. These effects depend on the type of organ, concentration, plant growth location, physiological maturity, etc.

An experiment was conducted to evaluate the allelopathic potential of wild barley (Hordeum spontaneum) weed on some germination, morphophysiological, and biochemical characteristics of a reference plant sensitive to allelochemicals, cress (Lepidium sativum), in 2021. This experiment was conducted in the form of a completely randomized design with three replications. The treatments included different concentrations of H. spontaneum aqueous extract at 11 levels (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100%).

The results showed that different concentrations of H. spontaneum aqueous extract had a significant decreasing effect on the percentage and rate of seed germination, radicle and shoot length, seedling length vigor index, radicle and shoot dry weight, as well as photosynthetic pigments (i.e., chlorophyll a, b, total, and carotenoids) of cress. Their effectiveness was dependent on the concentration threshold of the aqueous extract. In contrast, the content of proline amino acid , soluble sugars, ion leakage, catalase activity, guaiacol peroxidase enzymes, the concentration of malondialdehyde, and phenolic compounds showed an increasing trend compared with the control. In most cases, the highest increase in these compounds was obtained at the 100% aqueous extract concentration of H. spontaneum. This indicates the oxidative stress and high cytotoxicity caused by harmful compounds present in the aqueous extract of H. spontaneum.

According to the results of this experiment, it can be decucted that the aqueous extract of H. spontaneum, due to its allelopathic properties, prevented germination and seedling growth of L. sativum. Therefore, according to the proof of the harmful effect of H. spontaneum and its high biomass, it may be possible to introduce the bioactive compounds present in this plant for the production of environmentally friendly herbicides or even the potential to produce new formulations of synthetic herbicides in case its positive effect on other species is proven.

Experiments were conduccted to study the response of cress (Lepidium sativum) sensitive plant to allelochemical compounds to aqueous extract obtained from the different organs of important weeds such as Silybum marianum and Malva sylvestris. Experiment for each plant was performed as factorial based on the completely randomized design with three replications in 2023. The first factor was the different organs in three levels, namely root, stem, and leaf of weeds, and the different concentrations of the extracts obtained from each of the organs were at 5 levels (0 (control), 25, 50, 75 and 100%) as the second factor. To extract the samples, after collecting and drying them, first 5 g of the plant powder of each organ (weight) was extracted separately with 100 ml of distilled water as solvent (volume). Then different concentrations were prepared from the extract obtained from the stock (base solution). The results showed that the percentage and rate of germination, length of radicle and, plumule, length of seedling, ratio of radicle to plumule, seed length vigor index, dry weight of radicle and, plumule, seedling dry weight and seed weight vigor index of cress were influenced by aqueous extract of the Silybum marianum organs, different concentrations and interaction effect of them. Among the different organs, the aqoues extracts of leaf had the highest inhibitory effect on germination characteristics of cress. So that the difference in the effect of different concentrations of organs was dependent on their concentration threshold. As the findings showed that the effect of aqueous

In agricultural systems, several environmental stresses can remarkably alter the growth, physiological, and biochemical responses of plants under stress. One of these factors is the biochemical reactions between plants along with the production of secondary compounds. Allelochemicals mainly have defence and cell wall ligninization roles in plants and do not directly play a role in the growth processes of plants. Thus, an experiment was carried out to evaluate the effect of allelopathic stress of Hypericum perforatum on the germination, physiological, biochemical, and antioxidant activity characteristics of green pea, the benchmark plant sensitive to allelochemicals.

The treatments included different concentrations of H. perforatum at 11 levels (i.e., 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100% of the aqueous extract). This research was carried out as a completely randomized design with three replications at the weed science laboratory of Gonbad Kavous University in 2023.

The results of this study showed that one of the factors influencing the physiological, and biochemical characteristics of green pea is the concentration of the H. perforatum extract. In most cases, the percentage and rate of green pea germination, radicle and plumule length, and dry weight of radicle and plumule decreased with increased concentration of aqueous extract compared to the control, so that the greatest reduction in these characteristics was observed in 100% of H. perforatum extract. In contrast, the content of compatible osmolytes such as proline and soluble sugars, phenolic and flavonoid compounds, and antioxidant activity of green pea roots and plumules increased significantly in all studied treatments, with the highest increase in these characteristics observed at the concentration of 100% of H. perforatum aqueous extract. In general, the decrease in the dry weight of green pea seedlings due to the increase in the concentration of the aqueous extract of H. perforatum, despite the relative increase in the content of physiological and biochemical traits, indicates the high intensity of allelopathic stress of H. perforatum extract and their insufficiency, which leads to cytotoxicity against oxidative stress.

Considering the heterotoxicity effect of H. perforatum on green pea sensitive to allelochemicals and its distribution in gardens, barren lands, and wheat and corn fields, the possible effect of their residues in the next planting and even in case of presence in mixed cultivation should be considered.

The practice of growing two or more crops simultaneously in the same field is called intercropping and it is a common feature in traditional farming of small landholders. It provides farmers with a variety of returns from land and labor, often increases the efficiency with which scarce resources are used, and reduces the failure risk of a single crop that is susceptible to environmental and economic fluctuation. There is another important way that without incurring additional costs and use of water and fertilizer could result in higher production. This approach is increasing agricultural production per unit area by growing more than one crop in a year. Intercropping will be successful when competition for sources is less than competition within a species. Plants in the mixture can be chosen in a way that a species benefits from environmental changes caused by other species in mixed cultures directly. Intercropping inhibits the growth and development of weeds and leads to increased production. Since the system will reduce pesticide use, environmental pollution will be also less proportionally. The objective of the present study was to study some traits of chickpea (plant height, number of pods per plant, number of seeds per plant, number of seeds per plant, 100-seed weight, and plant yield,) under application of nitrogen and planting pattern of chickpea and quinoa.

In order to study the effect of nitrogen and intercropping ratios of chickpea and quinoa on some traits of chickpeas, a factorial experiment based on randomized complete Block design (RCBD) was conducted with three replications at Gonbad Kavous University farm during the 2020-2021 growing season. Nitrogen factor was included three levels of non-application and application of 25 and 50 kg N/ha and the treatments of planting pattern were included 9 levels of sole cropping of chickpea, 67 % chickpea + 33 % quinoa, 50% chickpea + 50% quinoa, 33% chickpea + 67% quinoa, 100% chickpea + 33% quinoa, 100% chickpea + 50 % quinoa, 100% chickpea + 67% quinoa, 100% chickpea + 100% quinoa and sole cropping of quinoa. In sole cropping, the distance between the plants on the row for chickpea and quinoa was 10 cm. The operation of harvesting the entire plot was done by removing the border rows and half a meter from both sides of the middle rows. For analysis variance of data software of SAS Ver.9.1.3 was used and treatment mean differences were separated by the least significant difference (LSD) test at the 0.05 probability level.

The results of analysis variance showed that planting pattern and nitrogen had a significant effect on plant height, number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight, and plant yield chickpea and also plant height, number of seeds per plant, 1000-seed weight, and plant yield quinoa. Plant height in additive intercropping was more than in replacement intercropping but the number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight, seed weight per plant, and pods weight per plant in replacement treatments and sole cropping of chickpea was more than additive treatments. Nitrogen application increased all traits. Plant yield of quinoa in replacement intercropping of 33% quinoa instead of chickpea was greater than other treatments. However, the plant yield of chickpea in sole cropping of chickpea and replacement intercropping of 33% quinoa instead of chickpeas with 14.11 and 13.26 respectively, which was greater than other treatments.

Plant height in additive intercropping treatments was more than in replacement intercropping but the number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight, plant yield, and plant dry weight in replacement treatments and sole cropping of chickpea was more than additive treatments.

The herbicides environmental pollution caused by their over-use is one of the most critical threats to ecosystems and human societies' health. Therefore, understanding the behavior of pesticides in the environment is necessary to reduce the adverse effects of the environment and optimize agricultural activities. The goal of this experiment was to evaluate the effect of manure level on the leaching depth of 2,4-D via soybean and cucumber seedling bioassay.

This factorial experiment was performed based on a RCBD with three replications. The first factor was livestock manure including control (without manure and herbicide), 0, 10, 25 and 50% of rotted manure all with herbicide and the second factor of soil layers considered as 0-2, 2-4, 4-6, 6-8, 8-10 and 10-12 cm. 2,4-D herbicide (72% L, 2 L.ha-1), 24 hours after soil saturation was applied. 48 hours after herbicide application, five seeds of the plant were planted in each pot and by the end of 30 days, shoot and root length and dry weight of shoot and root were measured.

The results showed that the 2,4-D leaching depth was 10 cm in soybean and 12 cm in cucumber without manure. Adding 10 and 25% manure did not show a significant effect on the 2,4-D leaching reduction, but treatment of 50% manure diminished the leaching depth in soybeans and cucumbers up to 8 and 10 cm. With 50% increase in manure due to increased herbicide adsorption in 2 and 4 cm layers, more inhibition was observed than other layers and as a result, less herbicide was transferred to the next layers. On the other hand, cucumber showed more sensitivity to different amounts of 2,4-D herbicide leaching than soybean, so that up to 8 cm layer, all treatments except herbicide-free control treatment indicated a significant reduction in measured traits. It seems that the increase of soil organic matter in the treatment of 50% manure in layers larger than 8 cm, improved the growth of measured traits close to the control treatment. According to the results of this experiment, cucumber can be used as an index plant to monitor the leaching depth of 2,4-D herbicide.

The more sensitive the plant is, the more it reacts to the herbicides, and as a result, the effective depth of leaching increases for that plant. At the present study, the effective leaching depth in cucumber and soybean plants was different. In soybean, it was between 6 and 10 cm, and in cucumber, depending on the measured trait sensitivity, it was between 10 and 12 cm. Cucumber showed high sensitivity to different amounts of leaching, so that up to a depth of approximately 6 cm in all treatments a significant reduction in measured traits occurred. Comparing the reaction of soybean and cucumber crops to the depth of 2,4-D leaching, it can be concluded that cucumber is more sensitive than soybean; So that in soybean from a depth of 6 cm and after, no sharp decrease in the studied traits is observed, but in cucumber up to a depth of 10 cm, the decrease was noticed. It is noteworthy that the addition of manure may reduce the leaching of herbicides, but at shallow depths due to the adsorption of high amounts of herbicides, and its gradual application will cause plant burns. Also, according to the results of this experiment, cucumber can be used as a benchmark in monitoring the amount of 2,4-D herbicide leaching.

In order to investigate the effect of Cenocarpus extract on the resistance of spinach to drought stress, a factorial experiment was conducted in the form of a randomized complete block design with two replications in the crop year 2019-2020 in the research greenhouse of Gonbad Kavous University. Experimental treatments including 5 levels of drought stress (0.10, 0.20,0.30,0.40 and 0.50 MAD) and 5 levels of extract (0, 2%,4%, 6% and 8%) were applied. The results showed that the greatest increase in consumption efficiency in the growth period and total water consumption was obtained from the 6% extract concentration with values of 1.045 μg/mL and 0.235 μg/mL, respectively. The efficiency of water consumption based on dry weight and the efficiency of water consumption on wet weight of the treatment without extract were obtained as 603.2% and 695.8%, respectively. The results of the comparison of the average weight of aerial parts under water stress and from the treatment of 2% extract were obtained. The maximum root length was obtained with 8.17 and 8.07 cm for 20% and 30% drainage, respectively, and the minimum root length belonged to the 50% moisture drainage treatment. The highest amount of chlorophyll, efficiency of consumption during the growth period and total water consumption were obtained from the control treatment, and also the highest efficiency of water consumption per dry weight and efficiency of water consumption per wet weight were obtained from the 2% extract treatment.

Rapeseed contains one of the highest quality oils with balanced components of saturated and unsaturated fatty acids. During the 2018-2019 academic year, the Bayekola Agricultural Research Station in Neka City conducted a randomized complete block design experiment with three replications. Factors included planting arrangement in two levels: one-row and two-row; and weed control in seven levels of application of herbicides: Treflan lontral herbicide 1 liter per hectare before planting and mixed with soil; butisan star 2.5 liters per hectare before planting and before emergence of rapeseed and weeds; lontral herbicide 1 liter per hectare in stages of 2 to 4 leaves of weeds; paraquat herbicide 2 liters per hectare in stages of 4 to 6 weed leaves; weed control and without weed control. The results showed that changing the arrangement of planting and application of guided herbicide in two rows increased canola seed protein percentage by 21.31%, linoleic acid by 24.22%, linoleic acid by 10.08%, oil yield and yield of canola by 78.46% and 78.48%, respectively. Also, palmitic acid decreased by 11.06% when application of this treatment was made. Therefore, by managing canola agronomy and adjusting the row spacing to utilize guided herbicide paraquat, in addition to weed control, canola also increased protein content, unsaturated fatty acids, oil yield, and rapeseed grain.

Barley is one of the most important crops in the world and ranks fourth in terms of nutritional importance and area under cultivation among cereals after wheat, corn and rice, but it has ranks first in terms of the extent of cultivation in a variety of climates. The world's food production is severely limited by environmental stresses. One way to sustain production for the world's growing population is to minimize the reduction in production caused by these tensions. Given that heat stress, in particular late season heat is the most widespread type of heat stress in the world, especially in the subtropical regions, and as a major constraint in the production of sustainable crop yields, including barley. Therefore, identifying heat-tolerant genotypes can be a valuable solution to this problem. The present experiment was undertaken to understand the impact of high temperature on yield and its attributing traits and to select heat stress tolerant parents and cross combinations for future breeding program.

To fulfill the requirement of this objective, the experiment was conducted in two environments i.e. normal and heat stress. Infact This study was performed to evaluate the heat tolerance at the end of the season and to identify barley tolerant genotypes using stress tolerance indices on experimental farm of the Gonbad Kavous University during the growing season of 2015-2016. The experimental plant materials were 120 barley genotypes which were evaluated in a randomized complete block design with two replications under two conditions without stress and heat stress. In addition, seeds of each genotype were grown in two meter rows with 20cm distance between the rows and the plant density was 270 plants per m-2.

The results of this experiment showed that the highest yield mean in non-stress conditions was related to genotype Torsh and in stress conditions was related to genotype Line143-2. The results of analysis of variance for yield trait showed that the differences between genotypes and the interactions of genotype and environment were significant at the level of one percent for the trait, which indicates high diversity and different reactions of cultivars and lines in different environments. Comparison of indices also showed that YI, STI, GMP and MP indices had the highest significant correlation with yield under non-stress and stress conditions and the superior indices of this experiment were for screening heat tolerant genotypes. Cluster analysis based on indices and yield under stress and non-stress conditions divided the studied genotypes into three groups.

Overall, the results of cluster analysis and principal component analysis showed that genotypes Torsh, Zarjo and 4Shori had the highest tolerance and genotypes Line105, Nosrat, Line98-2 and Fajr30 had the lowest tolerance to heat stress. Obviously, it is necessary to repeat the experiment in other places to be surer of these results and to obtain definitive results. On the other hand, to obtain heat-tolerant cultivars, it is recommended to cross the Torsh, Zarjo and 4Shori genotypes, and to locate heat-tolerant control genes, tolerant genotypes (Torsh and Zarjo) cross with sensitive genotypes (Line105 and Nosrat) can be used as two parents.

Chickpea is the third most important cereal crop in the world after beans and peas. European scientists found that studying 542 plant species in 26 European countries, including 125,000 time series, found that in 78% of the time between 1971 and 2000, the rate of development increased due to rising temperatures and shorter stages. The shortening of the growth period caused by the increase in temperature in most crops reduces yield because it absorbs less radiation and has less time for dry matter accumulation. The purpose of this study was to identify the effects of climate change on phenological traits of chickpea in Gonbad city in order to adapt and improve yield for future conditions.Materials and MethodsFirst, meteorological data from the Dome Synoptic Weather Station from 1993 to 2017, including daily data on maximum and minimum temperatures, sunshine hours and rainfall, and then processed and converted to DOY using the Srad -calc program (Soltani & Sinkler, 2012) Solar radiation was calculated based on sunny hours and days of the year. Then, new changes were made to the meteorological data based on climate change scenarios. The SSM-iLegume-Chickpea model was implemented for both rain and water conditions and each scenario. Planting type was selected according to the climatic conditions of the region, autumn and the type of free cultivar. Simulations For each rainfed and irrigated condition, three different planting dates (December, January and February) were selected and simulations were performed for 25 years (1993-2017). Results Planting date and climate change scenarios were significant at 1% level for all phenological traits in both rainfed and irrigated conditions, while the interaction effects of planting date and climate change except day to emergence were significant at 1% level. As the temperature rises in the future climate, the length of the phenological stages, ranging from day to flowering, day to pod start, day to physiological maturity, and day to harvest maturity will be reduced compared to normal conditions. Maximum decrease in phenology length was related to increase in temperature of 6°C and combined effects of doubling Co2 concentration, two percent decrease in precipitation and increase of 6°C under normal conditions, and minimum decrease in temperature increase scenario by 2°C. Co2 is a two percent decrease in recipitation and an increase of two degrees Celsius in both rainfed and irrigated conditions. Therefore, due to unchanged phenological stages under Co2 doubling scenarios and 2% reduction in rainfall compared to normal conditions, and reduction in phenological stages under the influence of temperature rise scenarios and similarity of the results of the temperature rise scenarios alone compared to the combined effects of Co2 doubling and 2% decrease in precipitation and an increase in temperature can be deduced that only an increase in temperature reduces the length of the phenological stages.ConclusionSimulation results in Gonbad city showed that increasing Co2 concentration in both rainfed and irrigated conditions and different planting dates had no effect on the phenological stages of chickpea but increasing temperature would significantly decrease the chickpea growth period, although this decrease in the length of chickpea growth is greater in the vegetative growth stage. As a result, the chickpea plant enters the reproductive stage faster, enjoys better temperature and humidity conditions in the reproductive stages, and causes premature and eventually evaporation of drought and late-season heat, and ultimately increases yield under conditions that will be dry. But delay in chickpea planting will shorten the chickpea growth period, depletion of available resources such as radiation, optimum humidity and temperature, dealing with end-season heat and drought, and ultimately yield loss.

The purpose of this study, allelopathic potential assessment of aqueous extract of root, stem, leaf and flower organ of Fumaria parviflora weed on morpho-physiological and biochemical characteractics of Lolium rigidum seedling in hydroponic culture. First, 5% suspension was prepared separately from the organs with help of distilled water and then the resulting suspension solution (extract) was applied on 7 day old of seedlings of Lolium rigidum. Based on the results, various organs of Fumaria parviflora had significant inhibitory effect on growth indices of root length and seedling dry weight, content of chlorophyll b and carotenoids of Lolium rigidum. In most cases, the most inhibitory effect was related to leaf and flower organ extract. But content of prolin, soluble sugar and phenolic compounds were increased compared to control. The greatest increase in these compounds were obtained under leaf and flower organs of Fumaria parviflora. Therefore, by identifying the components of Fumaria parviflora and experiment on other species, maybe be a candidate for production of natural herbicide or proposed a model for the synthesis of herbicides.

Rhizobium is terrestrial bacteria that can coexist and form nodules in the leguminous family. The legume-rhizobia symbiosis results in great quantities of nitrogen fixation throughout the world. Rhizobial populations become non-target microbiomes for the herbicide applied in the field. Various herbicides such as Trifluralin and Imazethapyr can affect legume-rhizobia symbiosis negatively or in hermetic way. Hormesis is a dose-response relationship phenomenon characterized by a low-dose stimulation and a high-dose inhibition. This study aimed to investigate the effect of Imazethapyr and Trifluralin herbicides at two pH value (5.5 and 7) on the growth of Mezorhizobium ciceri at in vitro conditions.

To investigate the effect of Imazethapyr and Trifluralin herbicides on M. ciceri bacteria, an experiment was conducted as a completely randomized design in Gonbad Kavous University. M. ciceri strain were grown and maintained on yeast extract mannitol agar medium. Yeast extract manitol broth medium was used at two pH equal to 5.5 and 7. Moreover, Imazethapyr (0.021, 0.042, 0.084, 0.168, 0.336, and 0.672 g.L-1) and Trifluralin (0.337, 0.675, 1.35, 2.7, 5.4, and 10.8 g.L-1) herbicides accompanied with no-herbicide control added to the medium containing of 105 cell.ml-1 of M. ciceri at in vitro condition and the measurement of bacterial population was calculated at 600 nm light absorption, using spectrophotometer. The experiment was carried out with 4 replications and repeated two times. Population trends at different herbicide were fitted using three and four parameters logistic models and the Brain-Cousens equation was used when the hormesis response was observed.

The bacterial population of M. ciceri affected by Imazethapyr and Trifluralin concentrations in pH=5.5 followed the three parameter logarithm logistic model and in pH=7 followed the five parameter Brain-Cousense model. The maximum bacterial populations in pH=5.5 and pH=7 were 22.8×106 from zero concentration and 378.5×106 from 0.021 dose of Imazethapyr, respectively. At 0.021 and 0.042 g.L-1 of Imazethapyr doses, the bacterial population increased by 25.6% and 10.25%, respectively, compared to the control, which indicates the bacteria's hormetic behavior. The amount of hormesis in Trifluralin from doses (0.337 and 0.675 g.L-1) was recorded equal to 1.01×108 cell.mL-1. The increasing percentage of bacterial population from these doses compared to the control was 20.92 and 16.14, respectively. The value of “e” parameter, which indicates the amount of herbicide required for 50% reduction of the bacterial population, in pH=5.5 was obtained from Imazethapyr equivalent to 1.03 10-1 g.L-1 and from Trifluralin equal to 2.93 g.L-1.

This study showed statistical evidence of M. ciceri growth stimulation against minimum doses of Imazethapyr and Trifluralin in neutral acidity as Hormesis. This bacterium's population growth reaction in both herbicides under acidic conditions followed the logarithmic logistic model, and no hormesis was observed. In general, with increasing the dose of herbicides used in this experiment, M. ciceri bacterial population growth decreased. However, depending on the culture medium's pH, the bacterial reaction process was different so that in neutral acidity and sub-lethal doses was accompanied by an increase in population growth and then as the doses increased the growth rate decreased.

One of the most important activities in eugenicity and maintaining the genetic resources in plants is recognizing the genetical variation and classification of the resources which are inheritance. In order to study the phenotypic variation of Echinochloa crus-galli L., seeds of 48 species from different regions of Golestan, Gilan, Mazandaran, and Bushehr were collected from rice fields and in a completely randomized design with three replications University of Gonbad-e Qabus were planted. Evaluations consisted of the total weight of panicle, the number of panicle, the main panicle weight, main panicle length, number of secondary branche, the main grain number, total grain weight, plant height, number of leaves, flag leaf length, flag leaf width, weight stems and leaves, flowering date and the number of tiller. According to the results of analysis of variance table all traits were significant at one percent level that reveal the diversity of species of E. crus-galli. In case of assessing genetical variation of 48 genotypes of Echinochloa crus-galli L., and in order to increasing the genetical loci of barley, we used 15 iPBS primers. This study took place in genetic and plant breeding laboratory of agricultural faculty of Gonbad Kavous University. From the 85 allels which were observed for all varieties, 40 pieces of them were polymorphism. The identified polymorphism allels observed by every primer, were 1 to 5 diverse allels (in average 62.66 allels for every section). The content of polymorphism of information (PIC) observed between 0.424 for the primer iPBS39 until 49.97 related to.

The purpose of this study was eavaluation of morphological and biochemical haracteristics of rice seedlings–Domsia cultivar to various concentrations (0, 0.024, 0.048, 0.076 and 0.1 mM) of extracted phenolic compounds of barnyard grass (Echino crus-galli (L.) Beauv). The experiment was performed based on a completely randomized design in three replications in hydroponic culture. Methanol was used to extraction of the phenolic compounds. In other experiment, different concentrations of hydroxyl benzene synthesis (0, 1.514, 3.054, 4.569 and 6.136 mM) also was applied on this cultivar. According to the results, effects of different concentrations of phenolic compounds of barnyard grass on charactristics of root length, width of largest leaf, relative water content of leaf, protein and total phenols of rice seedlings were significant at 5% probability level. So that, the extent of negative impact on these traits were increased with increasing concentrations of phenolic compouds of barntyard grass in comparison with control. The greatest reduction of these traits was related to 0.1 Mm of phenolic compounds concentration, although there was no significant difference between this treatment and some treatments. According to the results, significant reduction effect of synthetic phenolic compounds concentrations on studied traits in addition to leaf area in plant and content of chlorophyll a, b and total especially at higher concentrations was more than treatments of its natral concentrations in barnyard grass. Due to the observed negative impact of phenolic compounds of barntyardgrass on rice seedlings–Domsia cultivar and also exstance of considerable amount of aromatic groups of benzene derivatives such

Barley (Hordeum vulgare L.) is one of the four important cereals in the world. Soil salinity is one of the major barriers to the production of important agricultural products. Crops are one of the most important factors affecting the level of secondary metabolites present in plants under protective conditions. Secondary metabolites help plants to survive and survive external disturbances (such as pests and pathogens) and stress environmental conditions (such as drought or unfavorable soil conditions). Many agriculturally important traits are controlled by many genes and are known as quantitative. The regions within genomes that related to genes associated with a particular quantitative trait are known as quantitative trait loci (QTLs). The identification of QTLs based only on classic phenotypic evaluation is not possible. A major breakthrough in the characterization of quantitative traits that created opportunities to select for QTLs was initiated by the development of DNA markers. One of the main uses of DNA markers in research has been in the providing of linkage maps. Linkage maps have been used for identifying chromosomal regions that contain genes controlling simple traits and quantitative traits using QTL analysis.

In order to locate secondrey metabolits of salinity tolerant genes in barley in vegetative and reproductive stages, 106 F8 lines caused Badia and Kavir crosses was used and cultivated as augumented design.This research was conducted in the research greenhouse of Gonbad Kavous University in 2019 and 2020. The seeds of 106 lines as well as were parents planted in the pot. For salinity stress, the lines were kept normal until the end of the vegetative phase and then irrigated with 16 dS.m-1 in the reproductive stage. At the end of grain filling period, leaf samples were taken from flag leaf and secondary metabolites of sugar, phenol, catalase and peroxidase were measured. The linkage map was provided with markers with clear and consistent Mendelian segregation markers (152 SSR markers, 72 ISSR alleles, 7 IRAP alleles, 29 CAAT alleles, 27 Scot alleles and 15 iPBS alleles). Four methods of mapping CIM, ICIM, STMIM and STPLM were used to identify the control QTLs and estimate the effect genetic of one of them.

Gene loci were detected for the sugar content using CIM, ICIM and STPLME in the region of chromosome 1 at 26 cM and near the Bmaq0211. Also, for the peroxidase effective loci on chromosome 3 were identified in 44 cM flanked Bmac0067 and HVM33. The SMIM method was identified at position 118 cM between ISSR13-1 and ISSR16-4 for the sugar content, phenol, peroxidase of a gene locus on chromosome 4.

qSUG-4 QTLs (sugar content on chromosome 4) with a coefficient of 20.2 as well as qPHE-1 and qPHE-2 QTLs (phenol content on chromosomes 1 and 2) with coefficients of determination of 21.3, 29.21 and qPER-1, qPER-4b, qPER-5, qPER-7 (peroxidase on chromosomes 1, 4, 5 and 7) are a siutable candidate for marker-assisted selection programs in the barley recombinant line population