عباس روشن قصردشتی

Nowadays, bovine mastitis has been recorded as an inflammation of the mammary gland and Staphylococcus aureus is the main pathogens responsible for contagious mastitis in ruminants. Presented study is looking for to assess the existence of methicillin-resistant and PVL positive Staphylococcus aureus from bovine milk and if there is any relation among the detected genes and antibiotic resistance or not? For this purpose, 100 milk samples were collected from cows affected with mastitis from four dairy farms in Fars province. The samples were transferred to MSA medium and incubated at 37 °C for 24 h. The pure colonies were identified by biochemical as well as molecular techniques using mecA, mecC and PVL gene. Then, the antibiotic pattern of the isolates was analyzed based on CLSI 2019 and Whonet program. Finally, the results were analyzed using statistical analysis. Totally, 6 S. aureus strains were detected which was confirmed by biochemical as well as molecular techniques. The isolates showed different antibiotic susceptibility patterns, most of the isolates were multidrug resistant, while, the strain No. 5 was the most sensitive one. Our results indicated presence of mecA gene in strains 1, 2 and 3, mecC in isolates 4, 5 and 6, and the PVL gene only in strain 4. Hence, it could be concluded that although different phages transporting PVL genes to the S. aureus isolate, in this special research we didn’t find any relationship between presence of mecA, mecC, and PVL genes with antibiotics resistant pattern.

Urovagina is defined as the accumulation of urine in the anterior part of the vagina in cows. Urovagina in many cases may result in different types of uterine infections and these, in turn, cause in repeat breeding syndrome and infertility. The aim of the present study was to evaluate surgical correction of urovagina using modified McKinnon and cerclage of the vestibulovaginal junction techniques in dairy cows. Briefly, in the modified McKinnon technique, right and left sides of the vagina were joined medially and a large mucosa-lined tunnel was created. In the second method, vestibulovaginal junction under the vaginal wall cranial to the urethral opening was closed by creating the vestibulovaginal cerclage. The feasibility, possible side effects, success in management of post-operated cows and pregnancy rates were assessed. Twenty-three cows with urovagina were treated surgically using either a modified McKinnon (n=11) or cerclage of the vestibulovaginal junction technique (n=12). All cows were artificially inseminated after two administrations of a prostaglandin F2α analogue 14 days apart. Pregnancy diagnosis was performed by palpation per rectum 40–50 days following insemination. The mean numbers of inseminations before the urovagina diagnosis and after the treatments were 4.75± 3.1 and 3.09± 1.37 versus 1.25± 0.4 and 1.7± 0.67 (p<0.05) in cows treated with the modified McKinnon and cerclage of the vestibulovaginal junction techniques, respectively. The results of the present study revealed that both surgical techniques can be used successfully for the management of cases of urovagina in dairy cows.