محمدرضا عبداللهی

Wheat plays a very important role in meeting the food security in the world. The wild relatives of wheat have high genetic diversity for various traits. These traits can be exploited in order to improve the yield of bread wheat, and as a result, answer the question of food security for the growing population. Aegilops tauschii or Aegilops squarrosa L. is a diploid and self-pollinating species that has given the D genome to bread wheat as a donor parent. Genome D is an unexploited gene reservoir for genetic diversity that can be used to increase the quantity and quality of grain yield in common bread wheat. Aegilops tauschii as a valuable gene pool, has always been of interest to researchers to improve yield and related traits in wheat. Based on this, identifying and using allelic diversity for various root, phenological and morpho-physiological traits in this species is very important in order to expand the genetic base of wheat to improve wheat yield. Therefore, the aim of this research was to study the relationships between different traits and grain yield in order to identify the most important traits affecting the changes of grain yield in ten different ecotypes of Aegilops tauschii.

This research was conducted in a repeated manner during the years 2016 and 2017 in the Research Greenhouse of Bu-Ali Sina University, Hamedan, west of Iran, in the form of a randomized complete block design with three replications under normal moisture conditions (supplying 95% of field capacity). The plant materials of this research included 10 ecotypes of Aegilops tauschii. The seeds were germinated and grown in germination trays containing equal proportions of perlite and cocopeat. After the seeds germinated, all the seedlings in the trays were transferred to the cold room at the temperature of 4 degrees Celsius for five weeks in the two-leaf stage for vernalization. Then, the seedlings were transplanted into 10 kg plastic pots (pots had a diameter of 40 cm and a depth of 50 cm), which contained an equal ratio of agricultural soil, sand, and rotted animal manure. In this research, 34 attributes were measured and evaluated.

Results and Discution :

High genetic diversity among ecotypes was observed for most of the important traits related to grain yield. The desired ecotype A19 had the highest, and ecotypes A16 and A17 had the lowest yield and grain yield components. The results of correlation analysis revealed that an array of traits including grain water use efficiency, plant height, harvest index, grain weight per main spike, main spike weight, peduncle weight, spikelets/spike, peduncle length, thousand grain weight, grain filling period length, and main stem weight showed the highest positive and significant correlation (p≤0.01) with grain yield. Additionally, grain yield had the most significant (p≤0.01) and negative correlation with days to heading, water consumption, days to anthesis, root dry weight, and days to maturity. According to the stepwise regression results, water use efficiency, grain weight per main spike (both with a positive regression coefficient), and root dry weight (with a negative regression coefficient) were identified as the most important traits influencing grain yield variations. Water use efficiency had the highest direct positive effects on grain yield, while grain weight per main spike and root dry weight had the highest indirect positive and negative effects on grain yield, respectively, through increasing and decreasing water use efficiency. Therefore, it may be infered that due to the relative similarity of the genetic background of common bread wheat with Aegilops tauschii species, as one of its wild ancestors, the selection for higher grain water use efficiency and grain weight in the main spike, as well as root and phenological traits (except grain filling period) leads to the improvement of common wheat yield in normal moisture conditions. Ecotypes A16 and A17 were identified as the most unfavorable ecotypes, mainly because they needed greater number of days to heading, days to pollination, and days to physiological maturity, and invested more photosynthetic products to rooting attributes, including root dry weight. According to the results of this research, Aegilops tauschii, is an important gene reservoir for improving wheat yield. In this research, ecotype A19, where its higher amounts of grain yield and related traits, including water use efficiency and grain weight in the main spike are accompanied by lower amounts of phenological traits as well as lower dry weight of roots, was the most desirable ecotype.

Water use efficiency, grain weight per main spike and root dry weight were identified as the most important traits affecting grain yield. Therefore, it may be possible to select for higher values of water use efficiency and grain weight per main spike and lower values of phenological traits and root traits. These traits, which have high heritability and respond well to selection, can be used to identify ecotypes of this wild wheat. Ultimately, they can be used in future wheat breeding programs.

The ability to accurately measure inflation expectations is an integral part of the monetary policy of central banks in the world. In fact, according to the current monetary policy approach that is based on inflation targeting, central banks should be forward-looking and adjust their policies based on people's expectations of the future inflation rate. There are different methods to estimate inflation expectations, the most important of which is to measure inflation expectations by directly asking people about their expectations of prices. These proposed questions may be presented quantitatively or qualitatively. According to the quantitative or qualitative nature of survey questions, different methods are also used to calculate survey-based inflation expectations, which are generally divided into two categories: methods based on quantitative surveys and methods based on qualitative surveys. In this study, using the data obtained from the opinion survey of inflation expectations of the Islamic Council Research Center, which was conducted quarterly from the fall of 1400, the index of inflation expectations based on a qualitative survey in Iran from the fall of 1400 to the fall of 1402 has been calculated and analyzed. For this purpose, the methods of balanced statistics, probability method, and logistic function method were used. The results of comparing the inflation expectations calculated from the mentioned methods and the inflation rate realized in the next three months showed that the probability method with uniform distribution performed better in predicting the future inflation rate. Moreover, the analysis of the trend of the inflation expectations index in the last 9 seasons showed that the intensity of the increase in inflation expectations during the period of currency fluctuations was much higher than during the period when the preferred currency was removed. These results indicate that the central bank should prevent the occurrence of currency shocks and the significant increase in inflationary expectations as much as possible by properly managing the foreign exchange market, allowing the gradual increase of the exchange rate, and reducing its fluctuations around the equilibrium exchange rate.

Given the economic importance of growing flowers and plants in the world, the use of new technologies and methods in the improvement of ornamental plants in order to market them can play a significant role in marketing of these products and their trade in the international markets. Marigold (Tagetes spp.), is an annual and essential plant that is cultivated as an ornamental plant in many parts of the world (Neher, 1968). But in recent years, marigolds have been used as a commercial source of essential oils, biological compounds, pigments and cosmetics (Anonymous, 1976), control of malaria mosquitoes (Wanzala and Ogoma, 2013), antihypertensive, anti-inflammatory, antidepressant, antimicrobial, antimicrobial (Senatore and Feo, 1999), and control of nematode (Prasad et al., 1992). Anther or microspore culture is known to be an effective method for producing haploid plants (Henry and De Baizer, 1980). Hybrid seed production requires pure line (as a parent), and the double haploid method can reduce the production period of pure lines to 5-6 years. Production of hybrid seeds in this valuable plant is of great importance, and the double haploid method can be important in this regard.
 
In the anther culture of marigold, a culture medium containing 0.2 mg/l of Naphthalene acetic acid and 1 mg /l of 6-Benzylaminopurine was used. In this study, the effect of genotype, bud size and mannitol on androgenesis induction of marigold anthers was evaluated during two separate experiments. A factorial experiment was conducted in a completely randomized design with two factors including genotype at 5 levels (T1, T2, T3, T4, T5) and bud size at three levels (3-5, 5-10 and 10-15 mm) for first experiment. The second experiment was also performed as a factorial in a completely randomized design with 3 replications. In the recent experiment, the first factor included the method of mannitol application and the second factor included the different concentrations of mannitol (0.1, 0.2, 0.3, 0.4 and 0.5 M). The factor of application method was in 2 levels: 1: adding different concentrations of mannitol to the solid culture medium and 2: pre-treating the anthers with the liquid culture medium containing different concentrations of mannitol for 24 hours. The T4 genotype was used in the second experiment.
 
In the first experiment, the effect of different bud sizes and 5 different genotypes on callus formation, mean number of shoot per anther, mean number of shoot per callus and percentage of complete plant regeneration in anther culture of marigold were studied. The results of this experiment showed that buds with the length of 5-10 mm have anthers which their microspores are at the proper growth and development stage for callogenesis and shoot production. The T3 and T4 genotypes, (both of them belonging to French species, Tagetes patula), produced the highest percentage of plant regeneration among the various cultivars. In the second experiment, we explored the impact of mannitol treatment on androgenic traits in marigold anther culture. Specifically, we examined two concentrations: 0.1 M and 0.2 M mannitol, both applied in the form of solid culture medium. Additionally, we investigated two concentrations, 0.0 M and 0.2 M mannitol, when applied as a pre-treatment in a liquid medium containing mannitol. These treatments yielded the highest percentage of callus formation. While the pre-treatment of anthers with a liquid culture medium containing 0.5 M mannitol led to the highest mean number of shoot per anther and the mean number of shoots per callus. Also, the pre-treatment with liquid medium containing 0.2 M mannitol showed the highest percentage of complete plant regeneration.
 
Results showed that in marigold, buds with the size of 5-10 mm contained microspores with mid-uninucleate stage to early bi-nucleate stage showed the highest response to the induction of androgenesis. Also, T3 and T4 genotypes belong to the French species showed the highest response to the regeneration. In another experiment, the pre-treatment of anthers with 0.2 and 0.5 M mannitol by using mannitol in a liquid culture medium for 24 hours, respectively showed the highest percentage of complete plant regeneration and the highest mean number of shoot per callus and anther. Chromosome counting results showed that 3 out of 5 examined plants were dihaploid and had 24 chromosomes in their root tip cells, while examined mother plants were tetraploid and showed 48 chromosomes in their root tip cells.

The aim of this study was to evaluate the effect of limestone particle size and phytase enzyme supplementation on production performance, egg quality traits, and coefficient of apparent digestibility of calcium of commercial laying hens. A total of 216 laying hens were randomly distributed in six treatments, and six replicates per each. The design consisted of a factorial arrangement of three particle sizes of limestone from the same source (fine (<0.5 mm), medium (0.5-2 mm) and coarse (2-4 mm)) and two phytase levels (0 and 300 (FTU)/kg) in a completely randomized design. Egg production and egg mass were not influenced by experimental diets. Phytase inclusion numerically increased egg weight in second (P=0.06) and whole trial period (20-26 wk, P=0.1). Fine and coarse particles of limestone increased shell thickness (P < 0.01), compared to medium particles, also phytase supplementation improved shell percent (P < 0.01). Fine and medium sizes of limestone resulted in a higher apparent digestibility coefficient of Ca compared to coarse size ((P < 0.0001),0.43, and 0.45 vs. 0.33). Diets containing medium limestone size showed lighter yolk color than those containing fine and coarse particles (P˂0.05). Medium size of limestone significantly increased digestibility of DM compared to fine and coarse particles. In conclusion, productive performance of laying hens did not respond to limestone particle size, however, fine and medium sizes of limestone through enhancing effect on Ca digestibility, is more suitable than coarse particles for practical applications in young layers.

One of the aspects to consider in the health system is to focus on the core competencies of specialists in this field, which allows them to create significant differentiation from competitors by investing in their strengths. The purpose of this research is to design the model of core competencies of health system specialists in a hybrid method.

The present research was conducted using a meta-combination method in several stages in the health system. After identifying the concepts and codes of core competence components and evaluating its validity and reliability, the initial conceptual model of the research was designed. From the 53 retrieved studies, 7 studies related to the purpose of the study were included for meta-combining. At the level of analysis and presentation of the model, Sandelovski and Barso (2006) meta combination method was used.

The findings showed that the research model includes 9 components including participation and teamwork, health quality promotion, personal development, use of health information technology, system thinking skills, professional performance of responsibilities, evidence-based research and practice, profession E-ism was the observance of moral and legal procedures. The identified factors were approved by experts.

In order to focus on the core competencies in the health system, it is necessary to pay attention to the components of the core competencies of specialists and to institutionalize them in all parts of the health system.

This research aimed to study the relationship between different phenological traits with grain yield and investigate the effectiveness of these traits in single plant selection in quinoa, which was done based on a randomized complete block design. Grain yield per plant showed the most significant positive correlation with day-to physiological maturity and grain filling period and the most significant negative correlation with day-to-ten leaves and day-to germination.  Day-to physiological maturity, day-to-ten leaves (with negative coefficient), and grain filling period were entered into the regression model. The two traits of day-to-ten leaves and day-to physiological maturity had the most direct negative and positive effects on grain yield changes, respectively. The most indirect positive effect was related to the grain filling period, due to the increase in day-to physiological maturity. The distribution of genotypes in all four biplot areas indicated high genetic diversity of genotypes. The favorite genotypes (16, 8, 15, and 14) had the highest amount of traits of day-to physiological maturity, grain filling period, and day-to milky grain and were located in the favorable area of the biplot. According to the results of the cluster analysis, these genotypes were located in the "grain yield cluster". On the contrary, unfavorable genotypes (2, 7, and 12) had the highest day-to-ten leaves, day-to-germination, and day-to-four leaves. In general, the selection to increase the day-to physiological maturity and the grain filling period and the selection against the day-to-ten leaves and the day-to germination will improve the yield per plant of quinoa.

The purpose of this study was to evaluate the diversity and predict the response to selection in different traits of 16 quinoa genotypes, which was done in a randomized complete block design in Karaj and Kermanshah. The genotypes had a statistically significant difference (p ≤0.01) for the majority of the traits, and they were distributed in four biplot regions. Genotypes 14, 15, and 16 had the highest, and genotypes 5 and 6 had the lowest grain yield and grain harvest index. While the main panicle diameter, grain harvest index, day to physiological maturity, day to main panicle color change, 1000-grain weight, and day to flowering had the highest positive alignment with yield component, day to ten leaf trait had a strong but negative connection with it.  The highest genetic distance was observed between the first and third clusters, which can be achieved by crossing the selected genotypes of these two clusters, to create the greatest genetic diversity in the dividing generations. Grain harvest index and diameter of the main panicle with the highest coefficient of genetic diversity, high heritability, and the highest genetic progression were identified as two desirable traits with a high selection response.  Positive selection for these two traits and negative selection for day to ten leaf traits are suggested in future breeding programs of this plant. In general, due to the limitation of direct selection to improve grain yield, also according to the significant correlation (r = 0.994***) between the new parameter of "percentage difference between phenotypic variation coefficient and genotypic variation coefficient" with broad-sense heritability, the use of this new genetic parameter to select desirable agro morphophysiological traits is recommended.

Optimization of in vitro methods for the production of maize double haploids plays an important role in the breeding programs of this plant. In this study, the effects of 5-azacytidine on agronomic traits, androgenesis induction efficiency and also, DNA methyltransferase gene expression (AF229183.1) in two growth stages of maize were investigated. This experiment was performed as factorial based on a completely randomized block design with three replications. Two maize genotypes (DH5 × DH7 and ETMH-82) were considered as the first factor and treatment of maize seeds with 5-azacytidine (0, 5, 10, and 100 μM) was considered as the second factor. The maize seeds were sowed in the field and during the growth stages, various morphological and agronomic traits were recorded. In the anther culture experiment, the suitable anthers containing microspores at mid to late-uninucleate stages were selected and cultured in an YPm culture medium containing 1 mg/l 2, 4-D, and 2 mg/l BAP. Interaction effects of genotype and 5-azacytidine concentrations showed significant differences for the majority of studied traits except for number of kernel per ear row, kernel depth, plant diameter, number of leaves and number of ears. The highest amounts of 1000-kernel weight were obtained with treatments of 10 and 100 μM and the highest ones for grain yield and biological yield traits were obtained with 100 μM 5-azacytidine treatment for both genotypes. Seeds of DH5 × DH7 genotype treated with 5 μM 5-azacYtidine produced the highest mean number of embryo-like structures (0.1833) and regenerated plantlets (0.067) per each anther. Relative expression of DNA methyltransferase gene in maize seeds treated with different concentrations of 5-azacytidine showed a significant decrease in both genotypes and both growth stages compared to control plants (treated with 0 μM 5-azacytidine), that this decrease in gene expression could lead to improved androgenesis induction in anther culture of DH5 × DH7 genotype. However, despite the decrease in expression of this gene in two growth stages of ETMH-82 genotype, androgenesis induction was not observed in this genotype. The results of the present study can help to determine the role of epigenetic factors in androgenesis induction and improving the production of haploid plants in maize.

Caper (Capparis spinosa L.) is a rich source of rutin, plays an essential role in human health. In the present study, the effects of cold (25°C as control, 4°C, and 7°C for 2, 4, and 7 days), heat (25°C as control, 30°C for 14 days, 32°C for 2 and 4 days, and 35°C for 8 hours), and carbohydrate treatments on the androgenesis efficiency were studied in the anther culture of caper. Also, the effects of maltose and sucrose at the concentrations of 30 and 60 g L-1 in combination with two temperature treatments (1- 30°C for 14 days and 2- 7°C for 7 days + azacytidine and 2,4-D pretreatments) on the androgenesis induction was evaluated. The temperature and carbohydrate treatments showed statistically significant differences (p ≤0.01) in terms of callus and embryo formation. The 7°C for 2, 4, and 7 days produced the highest percentage (at the third week: 80, 78.34, and 76.67%, respectively) and callogenesis speed (7.85, 7.75, and 7.60 calli week-1, respectively) and the 7°C for 7 days produced the highest embryo production (0.57 embryo anther-1). The 30°C for 14 days treatment showed the highest percentage (at the third week: 100%) and callogenesis speed (9.44 calli week-1). While the 32°C for 2 and 3 days and also 30°C for 14 days produced the highest number of embryos per anther (0.22, 0.20, and 0.18 embryo, respectively). The use of 30 g L-1 maltose in combination with the 30°C for 14 days produced the highest percentage (at the third week: 91.66%) and callogenesis speed (8.94 calli week-1), while the 30 g L-1 maltose in combination with the 7°C for 7 days + azacytidine and 2,4-D pretreatments produced the highest mean embryo number per anther (0.55 embryo). The results of this research are of great importance for the use in the caper breeding programs.

Chickpea is one of the most important pulses in the world and it plays a key role in feeding of people in Iran. Iran is one of the original centers of chickpea. This plant has a significant genetic diversity and has favorable conditions for breeding and introducing new cultivars in Iran. According to FAO reports (2014), the average grain yield of chickpea in Iran with a cultivated area of 565 thousand hectares and an average yield of 557 kg per hectare is very low compared to the average of the major countries producing chickpeas. In order to solve this problem, identification of the initial variety of lines and cultivars to start the program is of particular importance. In fact, three factors of heredity, high diversity and severity of selection are effective factors in increasing the response to selection. In this regard, there are various methods for estimating genetic variation in plant species, one of the most important of these methods is multivariate analysis. It is necessary to use these methods for the classification of germplasm and the analysis of the genetic relationships existing between the modifying materials. Among the various methods of multivariate analysis, the principal component analysis, factor analysis, cluster analysis and decomposition function analysis are among the most important of these methods. In fact, one of the important goals of an outbreak is to select the best genotypes. In order to achieve this goal, the studied population should have a significant variation in terms of the characteristics studied, which knowledge of this diversity requires evaluation of the germplasm.

This study included 20 chickpea (Cicer arietinum L.) promising lines were planted in a randomized complete block design with three replications in 2013 growing season. To evaluate the diversity of lines based on important agro-morphological traits and to achieve the desired goals, various statistical methods including principal component analysis, factor analysis, cluster analysis and decomposition function analysis were used. Genotypic coefficient of variance (GCV) and phenotype coefficient of variance (PCV) using genotype variance and phenotype variance, respectively, were calculated. All calculations and statistical analyzes such as component analysis, cluster analysis, and decomposition function analysis were performed using SPSS and Minitab software.

The lowest and the highest coefficient of genotypic changes were related to day physiology (2.66%) and 100-seed weight (49.76%). The results showed that the three main first components explained 76% of the total variance of the traits totally. The first and second components were named as "component of grain yield increase" and "vegetative growth component". The results of factor analysis were consistent with the results of the principal component analysis. In order to cluster the studied lines and for their grouping based on the studied traits, Ward method and Pearson’s square distance matrix were used. According to the cluster analysis, the genotypes were located in three distinct groups, so that the second and third clusters had the highest genetic distance and they were recognized as “yield clusters" and "vegetative growth cluster", respectively. Therefore, crossing between the two above mentioned-groups, will result to high artificial diversity in the future generations. According to the cluster analysis, the lines were divided into three separate clusters. Discriminant function analysis confirmed the cluster grouping completely. In a study, nineteen chickpea lines and five genotypes of wild chickpea (Cicer veticulatum) were classified into three distinct groups using cluster analysis, so that wild-type genotypes in one group and two lines of cropping lines were grouped separately from other lines. Ganjali et al, (2009), applied the multivariate bi-plot analysis to evaluate the variation in chickpea germplasm for drought resistance.

There was a remarkable genetic diversity for the current germplasm, therefore existence a high level of genetic diversity and a remarkable GVC for the trait of 100-seed weight indicating that this trait can be used as a suitable character for yield improvement in the germplasm and under this experiment conditions. Finally, selection for high levels of biomass, pod number per plant and seed number per plant, and a low amount of main branches number, will increase the grain yield in the future breeding program.

One of the problems of crisis management in Iran is how to provide resources to carry out its activities in the face of severe economic sanctions. One of the economic and political tools to impose the demands of one country, in other words, to secure its interests by another country, is to use sanctions. In this case, the large and influential country in the world of politics and economics will be able to impose economic and political costs on the smaller country by imposing restrictions on the smaller economy and the less influential one. The association of other important economic and political actors of the world with the great provocative country takes on more serious dimensions. The same situation is assumed in the case of sanctions imposed on the Iranian economy. Sanctions are a series of actions that are applied without the use of threatening forces against the country, target or individual. International sanctions are penalties imposed in the context of the declared consequences of a failure to comply with international standards or requirements. Sanctions are instrumentally classified into economic and non-economic sanctions; Non-economic sanctions, such as not issuing visas to officials of the embargoed government in an international organization, opposing the embargoed countrychr('39')s offer to host important international events such as the World Cup, the Olympics, etc., can be achieved by means other than imposing economic costs. Economic sanctions have trade and financial aspects that in trade sanctions, prohibitions and restrictions on exports and imports are applied, but in financial sanctions, the sanctioner refrains from financial transactions, money transfers and investments, and uses his influence in International financial institutions prevent any technical cooperation, formal investment or lending agreements Seventhly, it should be noted that the most severe form of financial sanctions is the freezing or confiscation of the assets of the sanctioned country or their leaders. The main question that arises in this regard is what effect have the sanctions had on the health, public health and supply of medicine in Iran?

The present study was based on descriptive and analytical methods. According to the library research method, for collecting information after bibliography and collecting various political sources, filing tools and note-taking forms were used.

In order to explain the direct effects of sanctions on the current state of society, different theories and views by economists. Politicians and community health managers have been raised. The results of this study show that the health status, public health and welfare of citizens as well as the status of drug supply are directly affected by sanctions.

The results of the study indicate that economic sanctions have definitely always been associated with harmful effects that, without any distinction or distinction, harm the military and civilian forces, especially the vulnerable groups of countries. Difficulty and even lack of access to arbitrators, lack of currency transfer and the impossibility of providing drugs and in general the negative impact on the right to health and welfare of citizens and the inability of the government to ensure the right to health, the most important negative effects of sanctions on management The crisis is an unexpected event in Iran. The results of the paper showed that international sanctions have directly and indirectly affected the countrychr('39')s crisis management in the last decade. Sanctions, in particular, have created serious obstacles to the supply of needed medicines and the delivery of humanitarian aid. Sanctions on foreign exchange transactions and Iranian banks have severely restricted the opening of credit to importers of drugs and medical equipment, which has effectively led to the extension of sanctions to drugs and medical equipment. A number of pharmaceutical companies are refusing to sell drugs and medical equipment directly to Iran because of sanctions and to avoid the possible consequences of violating sanctions or even being barred from US markets. As a result, access to these detrimental items from international intermediaries at unconventional prices is restricted. Due to the expansion of sanctions on shipping companies and the lack of insurance for Iranian shipments, the cost of transportation to Iran has increased, which includes the increase in the cost of medicine and medical equipment, while there are reports of non-shipment, including drug shipments to Iran in There is a result of sanctions. The imbalance in the balance between the price of Rials and valid foreign currencies as a result of sanctions has led to an increase in the price of imported drugs and medical equipment, and as a result of the inability of the majority of patients to prohibit drugs.

Stevia rebaudiana Bertoni is from the dark Composite perennial plant, herbaceous and pollinator. Due to the being below seed germination perecentage and heterogenous multiplication of plants from seed in terms of sweetness micropropagation of this plant with use of tissue culture methods in order to replicate mass is desirable. In this study, the performance of two explant end bud and node using three treatments the concentration of the medium MS and different levels of activated charcoal and hormones BAP and Kn in two experiment compared and reviewed. So that in the first experiment the effect of active charcoal and concentration of culture media MS and in the second experiment use separate concentrations (at 0,0/5,1 and 1/5mgr/l) of hormones BAP and Kn in three repeat was implemented. Results showed the highest increase traits in the cultivation of end buds at full concentration seen of culture medium MS with to 1/5gr/l activated charcoal as absorber substance phenols. Use concentration 1/5 mgr/l of hormones BAP and Kn showed an increase in micropropagation efficiency of both explants. Performance comparison hormones BAP and Kn using 1/5 mgr/l BAP in the cultivation of the end buds showed the most characteristics of shoot length, number of nodes and the number of lateral stems.

Datura is one of the most important medicinal plants that for production of various alkaloids. Seed dormancy and the problem of germination are the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment with three factors based on completely randomized design with three replications. The first factor consisted of two datura ecotypes (Hamadan and Esfahan), the second factor included three levels of physical treatments of breaking dormancy (no scratching, scratch with sandpaper and wet bathing of bain marie bath for 7 days at a temperature of 60 ° C, scratch with sandpaper and dry chilling for 7 days at 4 ° C) and the third factor included five levels of chemical treatments of breaking dormancy (no application of chemical treatments, NaOH 20%, NaOH 20% and gibberellin 500 ppm, NaOH 20% and potassium nitrate 300 ppm, NaOH 20% and gibberellin 500 ppm and potassium nitrate 300 ppm). Interaction of ecotypes and seed dormancy breaking treatments was significant for most traits except for number of leaves per plant, root fresh weight, shoot length and stem fresh weight. The results showed that the chemical treatment of NaOH 20% and gibberellin 500 ppm, was the best dormancy braking treatment for the fresh harvested seeds. Therefore, using this treatment without significant damage to the seeds, to break seed dormancy about 80 percent is recommended.

Autumn sowing of sugar beet is a suitable way in sustainable agriculture. Bolting is an undesirable phenomenon which reduces sugar beet yield and it is the most important limiting factor in autumn sowing of sugar beet. Identification and comparison of the sequence of flowering genes in various genotypes can help to understand the molecular mechanisms controlling bolting. In the previous studies, it was revealed that expression level of FT1 and VIN3 genes in sugar beet is associated with bolting resistance. In this study, the sequence of FT1 gene promotor and three versions of VIN3 gene promoters of sugar beet were compared in three bolting resistant and three bolting susceptible genotypes. Primer design for each gene was carried out using the DNA sequences found at the NCBI database. DNA was extracted from leaf samples growing in pots and was used as template in PCR reactions. Similar length of amplified fragments for each promoter gene in bolting susceptible and bolting resistant genotypes were selected and sequenced for more accurate assessment. There was no mutation in the FT1 gene promoter, however 624 substitution and insertion/deletion mutations were observed in the promoter of three versions of VIN3 gene. A 228-bp ins/del region was detected in the VIN3-like1 promoter. This region contains promoter elements and seems to have a control function. Comparison of detected mutations between susceptible and resistant genotypes did not show a distinct pattern for bolting.

Payload placement on quad-rotor (QR) is a subject of considerable importance during its flight and maneuvering phases. In this sense, any changes in the payload position, especially along the vertical axis, could significantly affect the QR center of gravity (CG) position and, in turn, its performance, dramatically. The current experimental study investigates the effect of payload vertical positioning on the performance and stability characteristics of a typical QR. The QR is test flown for more than eighty times with different payload positions, whose recorded flight data (RFD) are filtered using extended Kalman filter and subsequently utilized for QR frequency domain analysis. The RFDs are used to identify the QR longitudinal and lateral modes. In addition, the mode changes trend against the center of gravity location has led to the determination of the CG position at which instability occurs. The experimental results show that as the QR CG moves up along its vertical axis, its dynamics modes move towards the origin on the real axis taking the QR closer to borderline dynamic instability. Moreover, the damping behavior of the longitudinal and lateral modes with respect to CG has been extracted that in turn can lead to CG based techniques for QR damping control. In addition, in order to generalize the present results to be useful for other QRs, the QR parameters have been nondimensionalized, and an effective nondimensional parameter through which results can be extended to other QRs is determined. The newly introduced nondimensional parameter is used against another set of data, extracted from a different QR for verification and comparative purposes. Finally, to account for uncertainties and stochastic noise in the RFD measures, each experimental stage is repeated four times; and the results of the mean behavior are reported, too.

Garlic has the second rank in Allium species in the case of highly used plant species after onion with high nutritional and medicinal values. Garlic is a vegetative product and is contaminated by many pathogens and viruses. Meristem culture is an in vitro bulblet production method for removing the viral diseases. The purpose of this study is to evaluate effect of growth regulators on micropropagation and bulblet formatiom in local clones of garlic in Hamedan province by meristem culture. This research was done as a factorial experiment in a completely randomized design with ten clones and four growth regulators including: MS, MS+1µM BA, MS+1µM BA+1µM NAA and MS+10µM BA+5µM NAA. After the first stage and plantlet production, a factorial experiment in a completely randomized design with 10 clones and 4 hormonal treatments was used in MS medium containing 6% sucrose with three replications. The cultured samples were placed in a refrigerator at a temperature of 4 ° C for four months and then transferred to the growth chamber with 25ºC temperature and photoperiod of 16/8 h (day/night). A month later, take notes of samples. In order to normalize the data logarithmic transformation and uniformity test of variances were used and then Data were analyzed by SAS software and the meanings were compared using Duncan's test. In the first experiment, the use of growth regulators in the culture medium causes significant changes in the production seedling growth characteristics. MS + 1μM BA medium produced the highest seedling weight and leaf length. Garlic clones significant difference in the number of leaves, leaf length and weight of seedling showed. Sheverin clone highest number of leaves, leaf length and the maximum weight the seedlings produced. Interaction between hormonal treatments in clones was not significant for any of the studied traits. In the second stage of the experiment, the interaction of clones in hormonal treatments was significant at 1% level for all traits (plantlet weight, Bulblet weight, Bulblet number, Root weight and Leaf weight). Sheverin clones in MS + 10μM BA + 5μM NAA were highest in all studied traits. totally, the results of this research showed that we can identify the responces of the top clones of the garlic province of Hamedan in meristem culture. Also we achieved the suitable concentration and combination of plant growth regulators on regeneration of different clone garlic in vitro conditions. As a result, a better protocoles were introduced for micropropagation and bulblet formatiom of the garlic in tissue culture.

The production of double haploid plants can be used as an effective method for plant breeding. In this research, in order to produce chickpea haploid plants, 1mm-long anthers of Bivanij cultivar containing the microspores at uni-nucleate stage were isolated from suitable buds (3mm in length) and exposed to different centrifuge (150g, 300g and 600g each for 3, 6 and 10 minutes) and electrical shock pretreatments (0, 100, 150 and 200V) in the 2ml microtubes containing 1.5ml of RM-IK medium. The treated anthers were then cultured in an EDM culture medium containing 10mg/l 2, 4-D and 10mg/l silver nitrate to induce callus and embryos. Results showed significant differences between different levels of centrifugation, different levels of electric shock and their interactions for the studied traits. The highest percentage of embryogenesis was observed in centrifuge pretreatments of 150g for 6 minutes, 300g for 3 minutes, 150g for 3 minutes in combination with 150V electrical shock, 300g for 6 and 10 minutes and combination of 150g centrifuge pretreatment for 3 minutes with electric shock of 200V, while the highest percentage of plant regeneration was obtained from centrifuge pretreatment of 300g for 6 minutes and also the combination of centrifuge pretreatment of 150g for 3 minutes with electric shock of 150V.

Production of pure lines is one of the most important tools due to production of high-yield seeds. Pure lines can be produced in a short time using in vitro techniques and therefore, reduce several years in required time for conventional plant breeding programs. The purpose of this study was investigation of different concentrations of TDZ on production of haploid plants in cucumber ovule culture. The experimental layout was conducted in factorial arrangement in randomized complete design with three replications in greenhouse and laboratory of Razi university during 2015-2017. Factors were two cucumber genotypes (Esfahani and Beit Alfa) and different concentrations of TDZ (0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08 mg/l). Unfertilized ovaries were harvested 1 day before an thesis and they were sliced into 1 mm cross sections under sterile conditions and placed on solid MS medium. Immediately after placing the unfertilized ovary slices of each genotype on induction medium, they were exposed to a thermal shock pretreatment at 35±1°C for 3 days. The first visual structures formed after 3 days in culture. After two weeks of culture, the frequency of embryo formation was recorded. After embryogenesis, the embryos were sub-cultured in medium containing 1.5 mg/L-1 GA3 and finally cultured in medium containing 0.05 mg/L-1 NAA and 1.5 mg/L-1 BAP for organogenesis. Results indicated a statistically significant effect at 1% among TDZ concentrations. Genotypes and interaction between genotype and TDZ concentration did not have a significant effect on embryo induction. According to the results of means comparison, M8 medium with the average of 23.33 for Esfahani genotype and 20.66 for Beta Alfa genotype had the highest embryo induction and M1 showed the lowest embryo induction for both genotypes. Ploidy level of calli and embryos were identified by Flucytometry assay. Haploid embryos were transferred to the regenerative medium. The chromosome content of haploid plants was doubled spontaneously in all regenerated plants. Double haploid plants originated from megaspore and they were equivalent to haploid, which relates to homozygosity and can be used directly in breeding program. The high number of embryos was obtained in high concentration of TDZ (0.08 mg/l). Flucytometry assay is a way to recognizing ploidy level in plant tissues; thus it could be useful in choosing haploid tissue. Spontaneous chromosome doubling may occur via somatic cell fusion, endoreduplication, endomitosis and possibly many other mechanisms. In conclusion, the higher rates of embryo formation frequency achieved and doubled haploids obtained in the present study are promising for future work.

High diversity and selection are base of each breeding program. The objectives of this research were assessing genetic diversity and clustering chickpea lines, estimating heritability and identification of the related traits to grain yield. The experiment carried out based on RCBD with three replications during 2012-13 growing season. The genotypes showed significant difference (p<0.05) for all traits and they distributed in four areas of bi-plot. The above results indicate that the lines had high genetic diversity. The broad sense heritability of traits varied from 60.17, for plant height, to 94.80, for 100-kernel weight. Three first main components recognized as "yield and yield component”, “plant growth or vigor component “and "water retain component” respectively and explained 73.3 percent of total data variance. The four main factors were respectively named as "yield and yield components factor", "increasing generative period factor”, "water retain factor” and "increasing growing period factor”. Above-mentioned factors explained 80.90 percent of total data variance. Cluster analysis grouped the lines in four clusters and discrimination function analysis confirmed the cluster analysis results. The lines of first and third clusters, had minimum and maximum yield and yield components respectively. Therefore, crossing between first and there clusters can be useful for creating new divers genotypes.

In order to investigate effects of plant regulators on callus and embryo induction in cucumber anther culture, 25 media for callus induction and 4 methods for embryo induction were tested. The experimental layout was conducted in factorial arrangement in randomized completely design with four and three replications for callus and embryo induction, respectively. Factors for callus induction were two cucumber genotypes (Esfahani and Beta Alfa) and different concentrations of BAP (0, 0.225, 0.45, 0.68 and 0.91 mg/l) and 2, 4-D (0, 0.25, 0.5, 0.75 and 1 mg/l). Also factors for embryo induction were different concentration of BAP (2, 3 and 4 mg/l) and NAA (0.05 and 0.1 mg/l) and different solid and liquid media. Results indicated that there are statistical significant effect at 1% among PGR concentrations and 5% among interaction between genotype and PGR concentrations. The anthers, which were cultured on basal MS medium and media supplemented with individual concentration of plant regulators did not show any response and they became necrotic after 4 weeks of culture, but combination of plant regulators had different effect on callus induction. According to results of means comparison M24, M22 and M15 media with average of 98, 97.75 and 96.75 percent and M10 medium with average of 77.5 percent had highest and lowest callus induction for Esfahani genotype, respectively. The highest and lowest callus induction for Beta Alfa cultivar obtained in M13 (98.45%) and M1 (59.7%) media, respectively. There was no response for embryo induction in Solid-Solid, Solid-Liquid and Liquid-Liquid methods, but high rate of embryo induction obtained in Liquid-Solid method. Results indicated that there was statistical significant effect at 5% among media. Means comparison for tested media showed that M6 (60.58%) and M4 (20%) medium had highest and lowest embryo induction, respectively.

Enough information about genetic parameters, including heritability and gene action, is the first step in breeding of a trait. Among different methods, generation mean analysis, with lower estimation error, is one of the best methods for estimation of genetic parameters. The present experiment was carried out to estimate heritability, number of genes and gene action for some agro-morphological traits in maize. The seeds of the generations of P1, P2, F1, F2, BC1 and BC2 were prepared during two successive years and were evaluated based on randomized complete block desine in Golestan Agricultural and Natural Resources Research and Education Center, Iran during 2015. Results of weighted ANOVA showed significant differences between the generations for all traits, therefore, generation mean analysis was performed. While, additive effect and relative dominance controlled ear length, number of row.ear-1, cob diameter and cob percentage, the additive, dominance and epistatic effects controlled the other traits simultaneously. Difference between dominance and additive components and the average of gene dominance was greater than unity which indicated the role of non-additive and the importance of heterosis effects in expression of these traits. The maximum heterosis and heterobeltiosis belonged to ear biomass and grain yield respectively. The broad and narrow sense heritability of the traits was ranged from 62.41 to 90.06 and 3.46 to 60.40, respectively. The average number of genes was ranged from 0.03 to 4.74 for different traits. In general, unlike grain yield, selection in early generations for yield-related traits with high narrow sense heritability such as ear length, cob diameter, 250-grain weight and number of grain.row-1, may improve grain yield in advanced generations. In addition, due to the high narrow sense heritability for cob percent (60.4%), selection in early generations for reduction in percentage of cob, with no significant increase the overall weight of the ear, may increase the ear harvest index and improving grain yield of maize.

To determine the type of genes action and estimate genetic parameters under moisture stress condition, two inbred lines of S0200237 (P1) and ILYH0231 (P2), sensitive and resistant to moisture stress, respectively, were crossed in 2013. Six generations P1, P2, F1, F2, BC1 and BC2 were planted in a randomized complete block design in 2015 and generation mean analysis was performed. Significant and positive heterosis was observed for ear biomass/ plant and ear-grain yield/plant. The joint scale test and the average of gene dominance confirmed the inadequacy of the additive-dominance model for most traits, indicating the importance of epistatic and over-dominant effect in genetic control of the traits. The results showed that row number/ear, ear diameter, cob diameter and grain depth were controlled by additive effect. The broad and narrow sense heritability of the ranged varied from 68.9 to 88.6 and 13 to 50.40, respectively. The average of genes number controlling the traits varied from 1-7 genes for grain yield. The maximum positive heterosis was observed for ear biomass and grain yield per plant. Generally, selection in early generations for grain yield-related traits with high narrow sense heritability, including the number of rows per ear, will improve grain yield in advanced generations. In addition, selection in early generations to reduce cob percent, to some extent, does not result in a significant reduction in total ear weight, and will result in an increase in harvest index, thus improves grain yield under moisture stress conditions.