دکتر ناصر عباسی

Dendritic cell-based cancer immunotherapy is considered an innovative and promising approach aimed at enhancing the host's immune response to combat tumors. Additionally, IPI-549 has been identified as a first-line therapeutic option for breast cancer treatment. The objective of this research was to develop and formulate a novel therapeutic supplement by combining dendritic cells with IPI-549 (Eganelisib) for breast cancer treatment. The concurrent administration of dendritic cells and IPI-549 (DC-IPI) was utilized to treat mice and elicit immunological responses triggered by vaccination. Tumor regression and overall survival rates were evaluated across five distinct experimental groups. The administration of tumor cell lysate alongside DC-IPI resulted in a significant reduction in tumor growth and a two- to three-fold increase in the survival duration of treated mice. DC-IPI, whether decorated with mannan or not, elicited stronger responses in terms of delayed-type hypersensitivity, lymphocyte proliferation, and CD107a expression. Moreover, our findings demonstrated a reduction in IL-4 production in the supernatants of splenocyte cultures. A significant reduction in BRCA1 mRNA levels was also observed following treatment with DC-IPI. In conclusion, our results suggest that the DC-IPI antigen delivery system exhibited substantial anti-tumor efficacy in a breast cancer mouse model, representing a potential advancement in immunotherapy for human breast cancer.

Every year, drug poisoning is the most prevalent reason for referring patients to medical centers. This study aimed to evaluation of morphine, methadone, digoxin, and dronabinol poisoning in Shahid Mostafa Khomeini Hospital in Ilam.

In this In this Cross-sectional study, patient samples suspected of morphine, methadone, digoxin, and dronabinol poisoning referred to the toxicology laboratory of Ilam University of Medical Sciences were analyzed using the HPLC method, and the results were analysed using SPSS software.

Results showed that the percentage of drug use is greater in men than in women. The highest percentage of morphine and methadone poisonings were detected in those under the age of 40, whereas the highest percentage of digoxin poisonings were recorded in those over the age of 80. As a result, the average age of digoxin users was substantially greater in men than in women. Methadone consumers showed significantly greater blood levels than others. In addition, there was a significant difference (P<0.01) in blood levels between men and women who used morphine.

In general, it is important to understand the status of drug poisoning with drugs such as morphine, methadone, digoxin, and dronabinol, as well as the prognosis associated with the treatment process of such poisoning.

The aim of the present study is to identify, report and compare native medicinal plants that are used for treatment of depression, anxiety and stress, anorexia, insomnia and headache and migraine in the Ilam city, Ilam province, Iran.

A standard questionnaire was used to obtain information regarding traditional therapeutic uses of plants that are effective on common psychiatric disorders, such as depression, anxiety and stress, anorexia, insomnia, headache and migraine. Data collection instruments included a questionnaire and interview. The study population also included 37 perfumers. First, a complete list of the perfumers of Ilam city was extracted from the Food and Drug Vice-Chancellor of Ilam University of Medical Sciences. The questionnaires included questions on the personal information of perfumers and a list that contains the no names of native plants, but included their used organs, usage, and traditional therapeutic effect. The results showed that twenty-two plant families are effective in treating common psychiatric disorders.

Fabaceae plant family with 7 plants and Lamiaceae plant family with 5 plants were the most effective plant families used for the treatment of common psychiatric disorders. Also, the most plant organs used for the treatment of the above disorders included aerial parts (38%), leaves (14%), fruits (14%), flowering branches (14%), and seeds (12%).

Native medicinal plants of Ilam city are traditionally used to treat common psychiatric diseases and can be used as natural and effective treatments of disorders such as depression, anxiety and stress, anorexia, insomnia, headache and migraine.

The aim of the study was to produce a Polyvinyl chloride-based membrane containing a polymer imprinted with a valproic acid molecule which was directly coated on a graphite electrode to determine the amount of valproic acid in aqueous samples. With the dispersion of molecularly imprinted polymer particles in dioctyl phthalate plasticizer as a solvent mediator, this potentiometric sensor was designed and after that it was embedded in a polyvinyl chloride matrix. The designed electrode showed a near-Nernstian slope of 54.1 ± 1 mV decade-1 in the concentration range of 10-6-10-2 M and a detection limit of 10-6 M with a response time of about 40 seconds for valproic acid and can be used for 2 months without divergence changing the potential. The fabricated electrode, in the pH range among 3.5-8.5, indicates the good sensitivity to valproic acid relative to the other ions. Finally, this electrode can be used as an indicator electrode in determining the concentration of valproic acid in valproic acid tablets.

Finding more efficient agents with fewer side effects for the treatment of burns has been a concern for researchers. The current study aims to assess Aloe vera (L.) Burm.f. and Vitis vinifera L. extract combination (AVEC) effects on wound healing in rats compared with silver sulfadiazine (SSD). Identification of individual polyphenols of the plant extracts was performed by HPLC. The animals were randomly divided into twelve groups. Standard second-degree burn wounds were induced on the back. Groups were treated with Aloe vera (A.V) and Vitis vinifera (V.V) creams at concentrations of 0.5, 1, 1.5, and 2% with ED50 values of 1.5 ± 0.02 and 1.4 ± 0.08 %, respectively. The other group was treated with an AVEC cream (1.5%). The samples of burned skin tissue were collected from the rats for histopathological examination. To evaluate the expression of VEGF and TGFβ1, the real time-PCR method was used. Kaempferol was only detected in Aloe. This study revealed that AVEC cream exhibits significant wound healing activity. VEGF and TGFβ1 had a significant increase in the AVEC. Based on our findings, AVEC cream can be a therapy of choice for burn injuries.

Enhancing microbial resistance to antibiotics and their probable side effects leads to the popularity of medicinal plants, so the need for novel antibacterial compounds with plant origin is felt more than ever. The object of the recent study was to assess the antibacterial property of Stachys lavandulifolia Vahl aqueous extract (SLVAE) on Escherichia coli O157:H7 (EC), Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA) and Bacillus subtilis (BS). The aqueous extract was obtained using a rotary evaporator. Agar disk and well diffusion methods were used to investigate the antibacterial property of the SLVAE. In the agar disk diffusion test, distilled water was used as a negative control whereas streptomycin, oxytetracycline, gentamicin, difloxacin, chloramphenicol, ampicillin, and amikacin were used as positive controls. Macro broth tube test was accomplished to specified Minimum Inhibitory Concentration (MIC). Statistical comparison among groups means were done through one-way ANOVA followed by Duncan’s post-hoc test. P ≤ 0.01 was considered as significant. Indeed compared with many standard antibiotics, the extract showed the higher antibacterial property. Also SLVAE with 125, 15/62 and 7/81 mg/ml concentrations has prevented the growth of EC, SA/BS and PA, respectively, and with 125, 62/5 and 15/62 mg/ml concentrations has destroyed EC, SA/BS and PA, respectively (p≤0.01). SLVAE had the most antibacterial activity on PA.  In conclusion the obtained results indicate the antibacterial effect of SLVAE on EC, PA, SA, and BS. It seems that this plant can be utilized for the treatment of some bacterial infections as an antibiotic.

Ethnomedicinal plants are considered recent resources for producing components that could treat wounds. Stevia rebaudiana is a native plant in Iran, which the plant has been used as an anti-inammatory, antioxidant, antibacterial, antifungal, antiparasitic, antiviral, and antidiabetic agent in Iran. In this study, the authors describe the property of aqueous extract of S. rebaudiana on wound healing markers in Sprague-Dawley male rats. Full-thickness excisional wounds (2×2 cm) was induced on the back of 32 rats. In this study, rats were randomly divided into four main groups (n=8), untreated (control) and treated with 1ml basal cream, 1ml tetracycline (3%), and 1ml S. rebaudiana aqueous extract 10% for 10 days. Animals of each group were euthanized at 10 days post-injury (DPI) and wounds were assessed through gross and histopathological analyses. Treated animals with S. rebaudiana demonstrated a considerable decrease in the wound area during the experiment compared to control, basal cream, and tetracycline groups. Parameters such as healing tissue alignment and epithelialization indicated substantial changes when compared to other groups. Moreover, treatment with S. rebaudiana decreased the number of wound surface area, lymphocytes and macrophages and it increased the number of blood vessels and fibrocytes compared to other groups at 10 days. Thus, the present research shows the wound healing effect of the S. rebaudiana, suggesting to use as a therapeutic supplement. Additional in vivo researches and clinical trials would be needed to justify.

Using a solid-phase extraction for the determination of VPA, this study aimed to prepare a valproic acid (VPA) imprinted silica gel adsorbent by surface molecular imprinting technique. VPA-imprinted silica adsorbent was characterized by FTIR spectroscopy. The VPA adsorption from solutions was studied in SPE mode. All influenced parameters on adsorption efficiency, including the elution concentration, MIP amount, VPA concentration, pH and salting effect, sample flow rate, column performance repeatability (relative standard deviation) were optimized independently. The prepared adsorbent showed significant adsorption capacity and selectivity for VPA. In the condition of sodium hydroxide solution (0.1 M), MIP polymer (250 mg), VPA (10-4M), sample flow rate (1 ml min-1), pH around 2-6 in the presence of 4 % NaCl, total VPA retention showed better reproducibility than 5%. Recovery of VPA was about 94.6 to 98.1% and the relative standard deviation was less than 5.69%.  These results demonstrated that the prepared adsorbent can be used to measure VPA in biological samples.

In this study, from June 2019 to May 2019 aerial parts of Scrophularia striata Boiss. were collected from Ilam city and those of Scrophularia deserti Delile from Dehloran city, Ilam province in western Iran. The aerial parts of the medicinal plants were pulverized. The essential and volatile oils were extracted by headspace solid-phase microextraction (HS-SPME) and their chemical compounds analyzed by Gas chromatography–mass spectrometry (GC-MS). Using HS-SPME, 68 and 49 chemical compounds were identified from S. striata and S. deserti, respectively. Spathulenol (18.41%) was the highest chemical compound of the essential oil of S. striata, followed by caryophyllene oxide (15.38%), linalool (11.85%), alpha-terpineol (8.34%), trans-caryophyllene (4.44%) and geraniol (3.10%). The most important identified compounds from S. deserti were caryophyllene oxide (15.38%), linalool (11.85%), alpha-terpineol (8.34%), trans-caryophyllene (4.44%) and geraniol (3.10%). The most important chemical compounds of S. deserti essential oil included alpha-pinene (25.54%), alpha-phellandrene (19.60%), beta-myrcene (11.29%) and trans-caryophyllene (6.78%). Results show major constituents of this two plant include with a high amount of Spathulenol and caryophyllene oxide has high pharmaceutical and healthful value.

Stomach ache is one of the most chronic and debilitating abdominal pains. Medicinal plants are one of the most accessible sources for treating diseases like gastrointestinal disorders. In this review, we investigate and report the most important medicinal plants recommended by Persian medicine for treating stomach aches and comparing them with their proven effects in modern medicine. Gastric pain was probed in Persian medicine reliable textbooks such as Cannon of Medicine (Ibn Sina), Tibbe-Akbari (Muhammad Akbar Arzaani ), The Complete Art of Medicine (Kitab Kamil as-Sinaa at-tibbiyya) (Haly Abbas), Explanation of insults and signs (Kermani) Tohfeh Al-Mo’menin (Seyed Mohammad Momen Tonekaboni), and some notes were taken. The results showed that Panicum miliaceum, Punica granatum, Solanum nigrum, Calicotome spinosa, Tamarindus indica, Cuminum cyminum, Prunus domestica, Matricaria recutita, Viola odorata, Plantago psyllium, Berberis vulgaris, Pyrus communis, Linum usitatissimum, Vitis vinifera were the most important plant sources used in treating the stomach ache according to Persian medicine. The findings of this study showed that the useful effects of many recommended plants in Persian medicine are confirmed by recent scientific researches and are reliable

Agonists of the Mu-opioid receptors (MOR), such as morphine are lengthily used for the treatment of moderate to pain, depression and anxiety. But the dose involved achieving adequate pain relief often elicits multiple unwanted side effects, including addiction and tolerance. Opioids produce their actions at a cellular level by activating MOR. These receptors are distributed throughout the central nervous system (CNS). The target of this study was the effect of Smyrnium cordifolium extract (SCE) on the hippocampus Cornu Ammonis 1 (CA1) aria MOR compared to clonidine.

Extract of the aerial parts S.cordifolium was extracted by Soxhlet method. Addiction was created using the subcutaneous injections of morphine for 7 days. To evaluate the effects of SCE, the mice were divided up 5 groups. The first group (Control) received just morphine. The 2th group received morphine and Clonidine (0.2mg/kg). Groups 3, 4 and 5 were treated morphine and SCE (E1, E2 and E3). In all groups, on the seventh day 30 min after naloxone injection, their brains were perfusion with formaldehyde and removed for immunohistochemical investigation.

The present immunohistochemical of the CA1 hippocampus study showed that group E1, there is a significant difference in MOR optical density compared to the control group at the level (P<0.05) and relative to the CLO group at the level (P<0.001). However, in groups E2, and E3 the MOR optical density increased compared to the control group and had a significant difference in level (P<0.001) and did not have a significant difference compared to the CLO group.

The study showed that with increasing S.cordifolium extract concentration, the optical density of MOR in the hippocampus increased, and this increase was dose-dependent. This increase in the CA1 hippocampus MOR optical density may be due to endocytosis or desensitization of MOR in neurons.

The involvement of secreted frizzled-related protein5 (SFRP5) and adiponectin, two important adipokines produced by adipocytes, in cardiovascular diseases demand further assessment. Therefore, in this study the relation of the adipokines and atherosclerosis was evaluated in Rat.

For the study, thirty male Wistar rats were divided into 2 groups (each group contain 15 rats): Control group, received a normal diet and the high cholesterol diet (HCD) group which received an additional 2% cholesterol and 0.5% cholic acid for 15 weeks. At the end of treatment, HCD-induced atheroma plaques were observed by hematoxylin and eosin staining of aortic tissue sections. Furthermore, serum levels of SFRP5 and adiponectin in the two groups of rats were measured by immunoassay and their relationships with the development of atherosclerotic plaques in the experimental group were analyzed.

The serum level of SFRP5 and adiponectin was significantly decreased in HCD rats compared with the control group (P<0.05).  There was also an inverse relation between the serum level of the two adipokines and atherosclerotic plaque formation (P<0.05). 

Serum levels of SFRP5 and adiponectin are decreased in rats fed with high cholesterol diet, highlighting the involvement of the two adipokines in atherosclerosis.

Spinal cord injuries (SCI) lead to permanent irreversible functional deficits. Poor prognosis of patients is the motivation of searching a treatment for the chronic injury. Planting stem cells provides us with a promising strategy. In the meanwhile, the use of olfactory ensheathing cells (OECs) has shown very good results. This study aims at evaluating the effect of transplanted OECs on functional recovery of acute SCI in rats.

In this study, eighteen adult male Wistar rats weighting approximately 210 ± 10 gr were used to study spinal cord injury. They were randomly divided into four groups: Sham (n = 3), Control (n = 6), Vehicle (n=3) and Treatment (n=6). In the Sham Group, only laminectomy was performed in the area of T9 spinal cord. In three groups of Control, vehicle and Treatment, after laminectomy, spinal cord contusion model was performed using the Weight drop technique. Immediately after the injury, 10μl Dulbecco's Modified Eagle Medium (DMEM) alone or with 106 cells were injected to vehicle and Treatment Group animals. For culturing cells, the olfactory mucosa of 7-day-old male Wistar rats was used. Motor function of animals in all groups, was evaluated in the first 48 hours daily and then weekly for eight weeks.

Comparing the results of the second to eighth week of the study showed significant differences in the group receiving the OECs with the control group (P<0.05).

The results indicate a positive influence of the olfactory ensheathing cells in functional improvement of spinal cord injury in the acute phase of injury.

The aim of this work was to evaluate the effects of four different concentrations of Aloe vera extract on healing of second degree burns in male Wistar rats.

By an experimental study 42 male Wistar rats were divided into 7 equal groups. The burn injury was made on the back of all rats, according to standard methods. The burned areas in the group1: were left without any intervention, group 2: were treated topically with eucerin, group 3: were treated with SSD and groups 4-7 were covered with 0.5%, 1%, 1.5%, 2% Aloe vera extract in eucerin (basal cream) twice a day, respectively. 21 days later the rats were sacrificed and samples of burnt skin tissue were collected for histological examinations. The parameters were evaluated to be considered for review included the number of hair follicles, sebaceous glands of angiogenesis, acute inflammation (severe infiltration of neutrophils) and the formation of epithelial layers.

Diagram of healing levels and reduction rats of wounds' sizes in 3th week indicated that the former was reduced more significantly in groups 3, 6 and 7 than in groups 1 (P<0.005) and 2 (P<0.01). Histological findings showed that burn healing was significantly better in groups 6 and 7 than the groups 1 and 2 at the day 21.

It could be concluded that concentration of 1.5% and 2% of Aloe vera extract have an effective role in the treatment of burn wound healing.