abdollah ghasemi pirbaloti

Lead as a heavy metal and permanent chemical contaminant threat the health of environment and alive creatures through different artificial and natural sources. In order to investigate the effect of methyl jasmonate and lead stress on growth characteristics, the adsorption and accumulation of lead and hazardous index in basil, a pot factorial experiment was carried out based on completely randomized design in research greenhouse of Ferdowsi University of Mashhad. The treatments consisted of three levels of lead nitrate (0, 200 and 400 mg/kg soil), and methyl jasmonate spraying in three concentrations (0, 0.5 and 1 mM). The mean comparison of data represented the reduction in all the studied morphological traits and increasing the amount of lead in the leave and root of the plant and hazardous index with increasing lead stress levels and spraying with methyl jasmonate could partly improve some of these traits. So that spraying with 1 mM methyl jasmonate cause to increase 6.01% inflorescence length and cause to decrease lead amount of the eave 96.56, 79.31 and 99.20%, respectively and hazardous index, however, at o.5 mM methyl jasmonate cause to increase the number of branches and aerial fresh weight, 27.66 and 9.84%, respectively. Therefore, it seems that application of methyl jasmnate on stress conditions could improve partly the traits and so in some cases 1 mM methyl jasmonate concentration cause to decrease some morphological traits. Therefore, application of o.5 mM methyl jasmonate on lead stress is recommendable.

Drought stress is as one of the most important limited factors that in agriculture systems can cause damage to growth, yield and secondary metabolites in medicinal and aromatic plants. A filed experiment to study on effect of irrigation frequencies, including 4, 6, and 8 every days on quantity and quality of the essential oil from the aerial parts of sage (Salvia officinalis L.) was done based on a randomized complete block design (RCBD) in three replications at the research field at Islamic Azad University of Shahrekord, southwestern Iran in 2016. The leaves of plant samples were harvested at early flowering stage and the essential oils were obtained by  hydro distillation by using the Clevenger-type apparatus d by GC/MS. Results indicated that irrigation frequencies levels had significant effects on essential oil yield and percentages of 1,8-cineole (as the main constituent in the oil), limonene and α–humulene. The highest yield oil and the maximum contents of 1,8-cineole (11.05%), limonene (2.67%) and α–humulene (1.32%) were obtained from treatment of eight every days of irrigation. Probably, it seems that increased irrigation intervals (mild drought stress) can be effective in quantitative and qualitative properties of essential oil from sage (Salvia officinalis L.).

Nepeta L., belongs to Lamiaceae family, is containing different annual and perennial species which are found in different parts of Asia, Europe and North Africa. In order to evaluate the morphological diversity, total phenolic content and antioxidant activity between 6 populations of Nepeta nuda and Nepeta crassifolia, the aerial parts of these plants in flowering stages were collected in summer 2015 from Ardabil and east Azerbaijan provinces. The morphological traits included plant height, number of sub-branches, leaf length and width, number of nodes, inflorescence length, internode length and length of sub-branch were measured. Methanolic extract were obtained by maceration, phenolic content by Folin-Ciocalto method and antioxidant activity were measurement DPPH radical scavenging capacity method, respectively. The experiment was performed in a completely randomized design with 3 replications and the comparison of data average was done by LSD test at 5% probability level. Results were showed that the first population (Nepetacrassifolia - Razi) was superior in morphological characteristics compared to other populations of this species. The highest antioxidant activity (74.96%)and total phenolic content (9.88mg of gallic acid equivalents per gram of fresh weight), was obtained from the leaves and flowers of population 3 (Heyran), respectively. Maximum plant height, leaf length and width, number of nodes were observed in population 6 (Meshkin) of Nepeta nuda and also the highest antioxidant activity and total phenolic content were perceived in leaves of population 5 (Heris) and flowers of population 4 (Meshkin-Heris), respectively. Generally the most total phenolic content and antioxidant activity between two species were observed in population 3 (Heyran) which is belong to the Nepeta crassifolia. It seems that the height of sea level had significant effect on populations phenolic content and antioxidant activity. So the populations of 1 and 6, which both species were desirable at evaluated morphological traits so that these populations can be considered in breeding and domestication programs.

Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was to investigate the cytotoxic effect of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge on Hela cancer cells line.
Hela cancer cells line and fibroblast normal cells line were cultured respectively in RPMI 1640 medium and DMEM medium with fetal bovine serum (FBS) and antibiotics. The cells exposed to different doses of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25 and 2.5 mg/ml) and were incubated for 24, 48 and 72 hours. After the incubation period, modified colorimetric MTT method was used to determine the cellular toxicity of the extract.
The results of the MTT test showed that hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge had dose-dependent and time-dependent anti-cancer effect on Hela cells line. The highest percentage of cell death was observed after 72 hours incubation and increase in extract concentration (p Hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge has cytotixic effect on Hela cells line. However this extract does not have any cytotoxic effect on fibroblast normal cells line. It seems that further researches can lead to detect effective compounds in the cancer treatment.

Cervical cancer is the most common   female genital cancer and the second most common cancer in women in the   world. Since the herbal ingredients have been used for centuries to treat   cancer, purpose of the present study was to determine the cytotoxic   effect of hydroalcoholic extract from Satureja bachtiarica Bungeon   Hela cancer cells line. Hela cells line and natural fibroblast cells line were cultured in RPMI 1640 and DMEM Containing fetal bovine serum and antibiotics, respectively. The cells were exposed to different doses of hydroalcoholic extract of Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25, and 2.5 mg/ml) and were incubated for 24, 48 and 72 hr, respectively. After the incubation period, the modified MTT colorimetric test was used in order to determine cell toxicity of the extract. The results of MTT test Showed that hydroalcoholic extract of Satureja bachtiarica Bunge has dose-dependent anti-cancer effect on Hela cancer cells, so that by increasing the concentration of extract and incubation in 72h, the most percentage of cell death was observed (p < 0.05). The extract of plant didn’t show any significant cytotoxicity on natural fibroblast cell line. Therefore, it seems that its compounds will be applied in treating cancer through more researches. Considering the low side effects of herbal medicines, especially antioxidants including Satureja bachtiarica Bunge can be used to treat cancer with drug therapy.