iraj posti

Diabetes mellitus decreases reproductive and sexual function in men by causing oxidative stress in testis cells and decreasing antioxidant enzymes. Several recent studies have found the effects of Cornus mas and Galega officinalis extracts on improving spermatogenesis dysfunction caused by diabetes. This meta-analysis study aimed to investigate the impact of G. officinalis and C. mas extract on testicular oxidative stress indices and sperm parameters in diabetic laboratory animals compared to healthy animals.

A search using Scopus, PubMed, Web of Science, and Google Scholar databases was conducted on studies that investigated the effects of plant extracts of C. mas and G. officinalis on testicular tissue and sperm indices in diabetic animals. The first part included the investigation of malondialdehyde (MDA) (nmol/mg), rate of catalase antioxidant activity, and superoxide dismutase. In contrast, the results of the second part included total sperm count (×106 ), total sperm motility (%), and immobility. The mean difference (MD) (95% confidence interval [CI]) was selected to express the effect size.

Out of 154 identified articles, four studies on mice were included in the meta-analysis. The random effect size yielded an overall effect size for MDA (6.96) with a 95% confidence interval [-6.09; 20.00]. The random effect size yielded an overall effect size for SOD of -0.5076 with a 95% confidence interval [-0.62; -0.40]. The random effect size yielded an overall effect size for CAT of -1.68 with a 95% confidence interval [-1.88; -1.47]. Also, a significant decrease in total sperm count showed the random effect size yielded an overall effect size of -24.74 with a 95% confidence interval [-30.73; -18.74]. The random effect size yielded an overall effect size for sperm motility of -26.65 with a 95% confidence interval [-29.54; -23.76]. A significant increase was demonstrated in sperm immobile in diabetic animals compared to the control group. The random effect size yielded an overall effect size for this indicator is 6.5157 with a 95% confidence interval [-1.96; 14.99].

G. officinalis or C. mas extracts have reduced effects on MDA levels and tissue damage and increased antioxidant enzymes in laboratory animals with diabetes.

In this research, we evaluated the protective effect of hydroalcoholic extracts of Galega officinalis and Cornus mas on spermatogenesis and oxidative stress associated with diabetes in the testes of adult rats.

In this experimental study, a total of 64 adult male Wistar rats were divided into eight equal groups (n=8 in each) as follows: (1) control group, (2) diabetic control group, (3) diabetic group receiving Galega extract daily at a dose of 50 mg/kg, (4) healthy group receiving Galega extract daily at a dose of 50 mg/kg for 14 days, (5) diabetic group receiving Cornus mas extract daily at a dose of 100 mg/kg, (6) healthy group receiving Cornus mas extract daily, (7) diabetic group receiving Cornus mas and Galega daily, and (8) healthy group receiving Galega and Cornus mas extract daily. Diabetes was induced by single intraperitoneal injection of streptozotocin (50 mg/kg). At the end of treatment period, all animals were anesthetized and blood samples were taken to measure the serum levels of insulin, glucose, and oxidative stress markers. Finally, the testicles and epididymis were removed and sperm parameters were assessed.

Galega and Cornus mas extract significantly reduced the oxidative stress, sperm parameters, glucose, and insulin plasma levels (P=0.001). Furthermore, the malondialdehyde (MDA) level was increased in diabetic rats. The activity of superoxide dismutase (SOD) and catalase (CAT) enzymes decreased in testicular tissue (P=0.001). Administering Galega and Cornus mas extract significantly improved these parameters (P<0.05).

Our results confirmed the antioxidant effect of administering Galega extract and Cornus mas extract on improving the sperm parameters and testicular oxidative damage caused by diabetes.