y. chashnidel

Today, the increase in the world's population, especially in third-world countries, has caused an increase in the demand for livestock products, which will subsequently require more livestock products and more food resources to meet these needs. The use of waste from factories of agricultural transformation industries in feeding livestock helps to make the cost of breeding more economical. Since cotton by-product is considered a product of cotton ginning factories, it can be said that due to its nutritional value, its use in animal diets is to avoid wasting this huge source of feed. Cotton by-product contains 86.9% dry matter, 12.3% crude protein, 57.7% NDF, 12.2% ash, 1.7% calcium, 0.31% phosphorus, and 1.06% net energy. The results of the studies showed that cotton waste can be used as a source of protein in the diet of fattening animals. This study aimed to assess the effect of feeding different levels of cotton by-products on performance, degradability, rumen fermentation parameters, microbial protein, and microbial population in fattening lambs.
In this study, 24 male Afshari lambs with a mean weight of 30±0.7 kg and a mean age of four months were used in a completely randomized design with four treatments and six replications for 90 days. The experimental treatments included: 1) a diet without cotton by-product (control), 2) a diet containing 33.33% cotton by-product instead of alfalfa, 3) a diet containing 66.66% cotton by-product instead of alfalfa, and 4) a diet containing 100% cotton by-product instead. Hay was based on dry matter. The cotton by-product used in this study was obtained from Mahosh cotton ginning factory located in Razavi Khorasan province, Neyshabur city, Iran. After entering the test site, it was chopped and then mixed with alfalfa in certain percentages; it was consumed by experimental lambs. Growth performance traits including daily feed intake, daily weight gain, and feed conversion ratio were measured during the experiment period. To measure ruminal fermentation parameters and bacteria and protozoa populations, the rumen fluid of the experimental lambs was taken from the rumen on the 90th day of the experiment, three hours after morning feeding using an esophageal tube. To determine urine purine derivatives, the total 24-hour urine production of each animal was collected in a special container under the metabolic cage on five sampling days. The number of three fistulaized Afshari sheep with a mean weight of about 50±2 kg and a mean age of approximately 11 months was used to estimate the parameters of degradability of dry matter, crude protein, and NDF.
The growth performance results showed that replacing 100% cotton by-products instead of dry alfalfa increased feed intake, increased daily weight, and decreased feed conversion ratio (P<0.05). The results of ruminal parameters showed that the treatment containing 66.66 and 100% replacement of cotton by-products instead of dry alfalfa had the highest concentration of total volatile fatty acids, acetic acid, and acetate to propionate ratio (P<0.05). There was no significant difference between the experimental treatments in the amounts of purine excretion derivatives and microbial protein production. The use of 66.66% of the substitute cotton by-product instead of dry alfalfa in the diet increased the total population of rumen fluid bacteria (P<0.05) but had no significant effect on the protozoan population. The results of meta-measures of dry matter degradability, crude protein, and NDF showed that there was no significant difference between experimental treatments.
The general results of the study showed that replacing cotton by-products by 66.66 or 100% instead of dry alfalfa in the diet improved the performance, increased the bacterial population, and concentration of total volatile fatty acids in the rumen fluid of fattening lambs. The use of cotton by-products can be a suitable substitute for alfalfa in the diet of fattening lambs without negative effects on growth performance and rumen fermentation indices.

By-products can have an important role in supplying livestock feed. Using these resources as a substitute for conventional resources decrease their demand for animal feed. One of the most common sources of demand is soybean meal. Optimal use of poultry by-products meal as a source of protein is one of the suggested solutions to decreases demand for soybean meal. Poultry by-products meal is said to be parts of poultry carcasses that have been milled if dry or wet, such as heads, feet, Intestines and viscera and except for feathers. Note that even in a good slaughterhouse there is a small amount of feather. The fat by more than 16% accelerates the Spoilage of this product, Therefore; it is recommended that the fat of these products should not be more than 10 to 12% (Gheshlagh Oliai et al. 2010; Watson 2006). Its chemical composition depends on the source of raw material, storage time of raw materials before processing, processing method, pressure and temperature during preparation and the amount of ash in the raw material (Jan Mohammadi et al., 1388). In order to use this by-product, the analysis of chemical composition and its effect on animal performance must be studied. The present study aimed to investigate the possibility of replacing protein sources conventional (soybean meal) with poultry by-products meal in the diet of fattening lambs and changes in growth, rumen and blood parameters, microbial population and microbial protein yield.

In order to investigate the effect of replacing the waste powder of poultry slaughterhouses with soybean meal, 16 male Dalagh mixed lambs with an average weight of 29 ± 0.5 kg were tested at the research site of the Animal Science Department of Golestan Agricultural Research and Training Center. The experiment was performed in a completely randomized design with 4 replacement levels and 4 replications. The diets of the lambs were adjusted according to the sheep nutritional requirements table (National Research Association 2007), with the same energy and protein levels. The lambs were fed a total mixed ration at two times on day with free access to water. Treatments (diets) included: control (soybean meal), 33% replacement, 67% replacement and 100% replacement of poultry by-product meal with soybean meal. Rumen fluid sampling was performed to measure ruminal parameters including volatile fatty acids, ruminal pH and ammonia nitrogen concentration through the stomach tube 3 hours after feeding. Determination of ammonia nitrogen concentration was measured by phenol hypochlorite (Broderick and Kang, 1980) and volatile fatty acids using gas chromatography. The culture medium PAC was used to count the microbial load (AOAC, 2005) and Protozoa count was performed by Dehoerti (1984) method. Protozoa were counted under a 40% magnification microscope with a neobar slide. Blood samples were taken from Neck veins at the same time as ruminal fluid sampling for the concentration of total protein, urea nitrogen and sugar of blood. measurement of microbial protein yield in the rumen was performed by estimating excreted purine derivatives by Chen and Gomez (1995) method in the end period.Urine was collected for 4 consecutive days. The sample collection containers contained 100 sulfuric acid to keep the pH below 3 and were frozen until the sample was tested.

At the end of the fattening period, no significant difference was observed in the gain characters: dry matter intake, weight gain of the period, average daily weight gain and feed conversion ratio. Changing the protein source to 10% of dry matter did not affect the palatability of the diet. Its efficacy for fattening indices has also been similar to that of soybean meal. There was a significant difference in blood urea nitrogen (P <0.05) and no significant difference was observed for other blood parameters (glucose and total protein). The comparison of the means between the treatments shows that treatment of 33% has a significant difference with the treatments of 66 and 100%. However, there was no significant difference between the control diet and 100% replacement. Also, blood urea nitrogen concentration was in the normal range. The correlation between NH3H and BUN and the lack of significant differences in blood glucose and protein concentrations indicate that the replacement of soybean meal with poultry by product meal in isocaloric and nitrogenic diets in 10% of dry matter has a similar effect on metabolism and physiology. Diet without poultry by-product meal has a higher bacterial population (P <0.01) and comparison of the mean effect of dietary protein source replacement levels on the protozoa population of ruminal fluid was not significantly different. The treatment of replacement 33% showed the highest level of ruminal NH3N (P <0.05). Acetate concentration was not significantly different between treatments and the concentration of propionate for treatment was 33% and 67% higher than other levels (P <0.01). Also, the ratio of acetate to propionate was significantly different (P <0.05). The pH of ruminal fluid for replacement levels was 33% and 67% lower than the control treatment (P <0.05). Replacement of the protein source in the diet with a constant content of nitrogen and carbohydrate concentrations did not alter the ruminal ecosystem. The effect of replacement levels on the mean of microbial protein production was not significantly different. Therefore, by replacing a similar nutrient in the diet and on the other hand, the internal conditions of the body and the tendency for homeostasis can be interfering in the synthesis of microbial proteins.

This study showed that the replacement of soybean meal with poultry by-product meal in lambs fattening diets up to 10% of dry matter did not have a negative response on fattening performance, blood and ruminal parameters. Also, it can reduce the demand for soybean meal in the rations.

 Lysophospholipids play an important role in animal nutrition. These substances, which are also classified as plant secondary metabolites, have a positive effect on the digestion and absorption of lipid nutrients in livestock. Lysophospholipids, mostly due to their emulsifying properties, increase the digestibility of fats and fat-soluble vitamins and can selectively prevent the growth of gram-positive bacteria. They can also emulsify dietary fats and increase fat absorption in the intestinal epithelium. The lysophospholipids can improve the consumption of fatty acid supplements and increase their digestibility. Therefore, they have substantial benefits for ruminants. The current study was conducted to evaluate the effects of consumption of different levels of lysophospholipids on degradability parameters, ruminal parameters, carcass fatty acid profile, microbial population, and protozoa in fattening male lambs.

 In this study, 24 crossbred male lambs with four treatments and six replications per treatment were used in a completely randomized design. The experimental diets included: 1. Basal diet (control) (without lysophospholipid in the diet and with a diet containing a lipid source), 2. Treatment containing a lipid source + 0.25% lysophospholipid in the diet, 3. Treatment containing a lipid source + 0.50% lysophospholipid in the diet, and 4. Treatment containing a lipid source + 0.75% lysophospholipid in the diet. The lipid source and lysophospholipid supplement were rumen-protected products. The total fattening period was 105 days (15 days of adaptation + 90 days of recording period).

 The results showed that adding 0.75% of lysophospholipid supplement increased feed consumption and daily weight gain and decreased FCR (P<0.05). With the addition of lysophospholipid supplement to the diet of fattening lambs, there was no significant difference between experimental diets with the control diet regarding the rapidly degraded fraction and slowly degraded fraction and total potential of degradability of dry matter, protein, and NDF (P>0.05). Experimental treatments had no significant effect on pH and ammonia nitrogen. The addition of 0.75% of the lysophospholipid supplement, compared to other treatments, increased the concentration of acetic acid and the ratio of acetate to propionate in the ruminal fluid and also increased linolenic acid (c18:3 ω3) (P <0.05) and the ratio of ω6/ω3 decreased in all experimental groups (P<0.05). The use of 0.5 and 0.75% lysophospholipid supplements in the diet increased the total ruminal bacterial population (P <0.05) but did not affect the population of protozoa (P<0.05).

 Based on the results of this experiment, it is possible to use lysophospholipid supplements at the levels of 0.5 and 0.75% of diets containing fat supplements in fattening male lambs.

Ruminal digesta is the nutrients consumed by the animal prior to slaughter, which is processed almost exclusively by rumination and exposure to the various enzymes and protozoa present in the rumen and a complete, ready-to-digest feed. After being processed, it is possible to re-feed it to ruminant animals. The use of digestive leftovers from slaughtered cattle could be a cheap, nutritious, and accessible alternative to the conventional feed ingredients. Therefore, the purpose of this study was to evaluate the effects of replacing cattle and sheep rumen digesta with alternative feed ingredients on the growth performance, carcass quantitative and qualitative traits, and some blood parameters of Dalagh fattening lambs.

A total of 24 Dalagh fattening male lambs at the age of 4.5 months, with a mean live weight of 27 ± 2.0 kg, were assigned to 6 treatments and four replications in individual cages for 90 days. Experimental treatments consisted of: 1) control, without rumen digesta of cattle and sheep, 2) 15% rumen digesta of cattle, 3) 30% rumen digesta of cattle, 4) 15% rumen digesta of sheep, 5) 30% rumen digesta of sheep, and 6) 15% rumen digesta of cattle, and 15% rumen digesta of sheep.

The results showed that daily gain and FCR were significantly different between treatments at the end of experiment (P <0.05). The highest daily weight gain and final body weight as well as the improving feed conversion ratio were observed in the treatment containing 30% rumen digesta of sheep. The results of body biometric showed that body length was significantly higher in the treatment contaning 30% rumen digesta of sheep, compared to the control (P <0.05). The results of quantitative carcass traits showed that treatment containing 30% rumen digesta of sheep in hot carcass weight, cold carcass weight, and crustacean were significantly higher than the control treatment (P <0.05). The results of some blood parameters of day 90 showed that there was a significant difference in the concentrations of glucose, cholesterol, BUN, and total protein between the experimental treatments (P <0.05). Therefore, in the treatment containing 30% of rumen digesta of sheep, the concentration of desired parameters was higher.

The results of this study showed that the use of rumen dried digesta in the diet of fattening lamb was effective in improving growth performance; especially live weight gain, FCR and carcass traits. Consumption of dried rumen digesta of sheep also had a significant effect on some blood parameters of experimental lambs. Similarly, 30% of rumen digesta of sheep had a better performance than other levels.