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In this study antimicrobial effects of Salvia mirzayanii and Salvia atropatana were evaluated against six gram-positive and gram-negative bacteria by immersion bioautography, cylinder plate and tube dilution methods separated. In bioautography method, essential oils of Salvia mirzayanii and Salvia atropatana were separated on silicagel TLC plates by toluene-ethyl acetate (93-7). In cylinder plate and tube dilution method, methanolic (80%) and aqua extracts were taken by maceration method. After concentrating the extracts, they were dried, then the 50, 25, 12.5, 6.25, 3.125 and 1.562 mg/ml distilled water solution of the dried were used for searching antimicrobial effects. Essential oil and extract of Salvia mirzayanii against E.coli, S.aureus, K.pneumonia, B.subtilis and P.aeroginosa in different dilutions were effective and essential oil and extract of Salvia atropatana against E.coli, S.aureus, S.epidermidis and B.subtilis were effective too.