جستجوی مقالات مرتبط با کلیدواژه « آنزیم لیپاز » در نشریات گروه « زیست شناسی »

Fungi are important sources of commercial and applied enzymes in various industries. The aim of this study is to introduce an optional salt-loving strain that produces cellulase, lipase and pectinase enzymes and optimize enzyme production using Taguchi method.

In this study, more than 350 fungal samples were isolated from the soil and climate of Tehran Forest parks and identified. After qualitative and quantitative analysis, 19 strains that had the highest production of lipase, pectinase and cellulase were identified by molecular technique. Then, based on the amount of enzyme production, optimization and design of Taguchi statistical method were performed by 8 factors at three levels to produce two cellulase and lipase enzymes for the best strain.

About 280 isolates were identified, including the genera Aspergillus sp., Penicillium sp., Fusarium sp., Mucor sp., Rizhopus sp. They secreted most of the enzyme’s pectinase, lipase, protease, amylase and cellulase. Among the isolated samples, Penicillium halotolerans had the highest enzyme production, which was selected for optimization. The production of cellulase and lipase enzymes of this fungus increased before 0.0744 and 0.0233053 U / ml before optimization and after optimization, increased to 0.63872 and 0.192105 U / ml. The most effective factor in the production of both enzymes was temperature and nitrogen source. The production of pectinase enzyme in the initial absorption of 540 nm was 0.71 (U / ml) which was not included in the optimization.

Optional salt-loving fungi can be introduced as a commercial and industrial strain due to their stability in fermentation conditions, availability, cheapness and locality.

  Today, the ability to produce hydrolases enzyme that are active in high salt concentrations is considered a new approach to the use of halophilic bacteria in biotechnology. The aim of this study was the screening and isolation of extracellular lipase producing halophilic bacteria Marinobacter sp. S-14 isolated from Badab-e Surt Hypersaline spring. In this experimental study, 42 pure bacterial colonies were isolated from different samples of water, soil, sediment, and sludge from a hypersaline spring with a screening technique on the specific culture medium of halophilic bacteria. The isolate S-14, which showed the highest lipase activity, was selected for the identification by biochemical methods and 16S rRNA gene analysis. In order to optimize the growth conditions of the isolate, considering the maximum time of bacterial growth (72 hours), temperature, salt concentration, pH, carbohydrate, and amino acid intake were examined. The results were edited by Chromas pro 2.1.1 software, and compared with EzTaxon database. Strains that were more similar to the isolate were identified. Sequence analysis of 16S rRNA were performed by BioEdit 7.1.9, Clustal-2X 2.1, and MEGA 6, and the phylogenetic tree was drawn by the neighbor joining algorithm. The isolate S-14 had 99% similarity to Marinobacter flavimaris and Marinobacter adhaerens. The isolate had optimum growth in 5% NaCl concentration, 35°C, and 7.0 acidity. The isolate S-14 can be an appropriate candidate to produce extracellular lipase enzyme and can utilize Fructose and Phenylalanine as a sole source of carbon and energy.