اسید هیومیک

In order to investigate the effect of nitrogen fertilizer and foliar spraying of humic acid and Vita free on phenological stages, remobilization indices and yield of sunflower in the second experimental planting in a factorial manner based on a randomized complete block design with with three replications in summer 2018 at Gonbad Kavous University farm research. Cultivar factor was included Haysan 25 and Oscar and combination of fertilizer factor was included no fertilizer application, 100 kg N/ha, 50 kg N/ha, 50 kg N/ha + 1.5 kg humic acid/ha, 50 kg N/ha + 0.5 kg Vita free/ha and 50 kg N/ha + 1.5 kg humic acid + 0.5 kg Vita free/ha. The results showed that the effect of cultivar and fertilizer on most of the measured traits was significant. The results showed that cultivar and fertilizer were significant on most of the studied traits. The maximum days from planting to 50% Flowering, the dry matter of vegetative organs in the flowering stage, the remobilization, current photosynthesis and seed yield were obtained from Oscar cultivar. The maximum remobilization and remobilization efficiency were obtained in the treatment of no fertilizer use. Days from planting to 50% flowering, days from planting to 50% maturity, current photosynthesis and seed yield in 100 kg N/ha treatment and 50 kg N/ha + 1.5 kg humic acid/ha + 0.5 kg Vitafree/h was higher than other fertilizer treatments. Based on results, in order to achieve the maximum seed yield of sunflower and to prevent excessive use of chemical fertilizers, the combined use of Nitrogen fertilizer, humic acid and vita free is necessary.

In order to study the effects of mycorrhiza and humic acid on compatible osmolytes, stomatal conductance, antioxidant enzyme activity, and some traits of bread wheat under water limitation conditions, an experiment as factorial was conducted based on a randomized complete block design with three replications at the research farm of the Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2018-2019. Factors experiment included irrigation at three levels (full irrigation, irrigation withholding at 50% of heading and booting stages as moderate and severe water limitations (BBCH 43 and 55, respectively) and application of mycorrhiza and humic acid at eight levels (application of Glomus intraradices, Glomus moseae, both applications G. intraradices and G. moseae, humic acid, humic acid with G. intraradices, humic acid and G.moseae, humic acid with G. intraradices and G. moseae, without humic acid, G. moseae and G. intraradices as control). Means comparison showed that both application of G. intraradices and G. moseae with humic acid increased stomatal conductance and relative water content (60 and 47.1%, respectively) compared to no application of mycorrhiza and humic. Also, under severe water limitation conditions, the activities of catalase, peroxidase, and polyphenol oxidase enzymes, along with grain yield, increased by 38, 25.4, 83 and 24% as compared to the uninoculated plots with no humic acid application at the same irrigation level.

This study was performed to investigate the effect of mycorrhiza fungus and different levels of humic acid (zero, 200 and 400 mg/l) and salicylic acid (zero, 100 and 200 mg/l) on physiological characteristics and essential oil content in three cultivars Lemonella, Citronella and Varigated. Lemon balm (Melissa Officinalis L.) was implemented in 1397 in a greenhouse in northern Iran. The experiment was performed as a factorial in a completely randomized statistical design with 3 replications. Mycorrhiza and humic acid treatments were applied at the time of seed sowing and salicylic acid was sprayed in two stages (second and third weeks of pot cultivation). The results of this experiment showed that all treatments had a significant effect on the measured variables. The highest proline content was obtained in Citronella cultivar and control treatment and The highest phenol, carbohydrate, essential oil percentage in the treatment of mycorrhiza fungus+ humic acid 400 mg/l + salicylic acid 100 mg/l were obtained in Varigated, Citronella and Lemonella cultivars, respectively. Also the highest amount of flavonoids in the treatment of mycorrhiza fungus+ humic acid 400 mg/l + salicylic acid 200 mg/l in Citronella cultivar and the highest total antioxidant activity in the treatment of mycorrhiza fungus+ humic acid 200 mg/l + salicylic 200 mg/l in Lemonella cultivar was observed. In general, the results of this experiment showed that the application of mycorrhiza fungus with humic acid 400 mg/l and salicylic acid 100 mg/l in Lemonella, Citronella and Varigated cultivars improved the physiological characteristics and essential oil of Lemon balm.

In order to investigate the effect of humic acid foliar application on some morphological and phytochemical characteristics of Physalis peruviana L., a factorial experiment was carried out under complete randomized design with three replications at the research greenhouse of Nahavand Higher Education Complex in 2021. Experimental treatments included humic acid foliar application (0, 300, 600 mg.L-1) and harvesting stages (green fruit and red fruit). The results showed that the highest amount of fresh and dry weight of leaves created in the treatment of 600 mg.L-1 humic acid and first stage of harvesting (green fruit). The highest amount of fruit fresh weight was obtained in the treatment of 600 mg.L-1 of humic acid and in the second stage of harvesting (red fruit). Also, the highest amount of fruit dry weight and the number of leaves was obtained with the application of the highest amount of humic acid (600 mg.L-1) in the second stage of harvesting, which had not statistically significant different with the treatment of 300 mg.L-1 of humic acid. The highest amount of leaf scopolamine was obtained in the treatments of 600 mg.L-1 of humic acid and the first stage of harvesting, as well as the treatments of 300 and 600 mg.L-1 of humic acid and the second stage of harvesting. The highest amount of fruit scopolamine was obtained in the treatment of 600 mg.L-1 of humic acid and the first and second stages of harvesting. The highest amount of atropine was created in the leaf with the application of 600 mg.L-1 of humic acid and in the fruit with the application of 300 mg.L-1 of humic acid. In order to reduce the use of chemical fertilizers, prevent environmental pollution and achieve sustainable agricultural goals, it is suggested to use humic acid as an alternative to chemical fertilizers in field studies.

Eryngium campestre is a biennial herbaceous plant native to northern Iran.  Nowadays, the use of chemical fertilizers has increased significantly, which has irreparable effects on the environment and human health.  The use of organic compounds and biofertilizers instead of chemical fertilizers has recently received much attention. Also, it is more important to study about natural substances that contain more antimicrobial properties and less side effects, such as plant extracts. For this purpose, an experiment was conducted. This experiment was conducted in Gorgan University of Agricultural Sciences and Natural Resources in 2019-2020 in a factorial format based on a completely randomized design with 3 replications. The treatments included: nitroxin (0, 1 and 2 ml/l), humic acid (0, 1 and 2 ml/l), glycine (0, 75 and 150 mg/l). The measured traits included total phenol, total flavonoid, antioxidant activity, total sugar, chlorophyll, carotenoid and activity of catalase and peroxidase enzymes. The effect of glycine on the measured traits was significant except flavonoids and catalase enzyme activity at 5 and 1% levels. Also, the effect of nitroxin on the measured traits was significant at the level of 1%. The effect of humic acid on the measured traits was significant except for the amount of antioxidant activity, chlorophyll and catalase enzyme activity at the level of 5 and 1%. Also, the interaction effect of all three treatments on the measured traits except catalase enzyme was significant at 1% level. In general, the results showed that the treatments used in this research, individually and in combination (interaction effect), increased most of the measured traits. Also, an experiment was conducted regarding the effect of Eryngium campestre extract on the population of Pseudomonas aeruginosa and Staphylococcus aureus bacteria. The results showed that Eryngium campestre extract did not inhibit the bacteria. In general, the results showed that the used compounds can be used to improve the biochemical characteristics of the Eryngium campestre.

In order to investigate the effects of using some growth biostimulants in low irrigation in pomegranate (Ghojagh cultivar), a two-year experiment, from March 2018, in a split plots design and in randomized complete blocks array, was carried out in Qom. The main plots included irrigation based on the irrigation SWAT model and 70 percent of it. The sub-plots were1-control (based on soil test), 2-treatment 1+ foliar application of amino acid in concentration of 5 gram.lit-1, 3-treatment 1+ soil-use of humic acid, 4-treatment 1+ foliar application of folvic acid in concentration of 5 gram.lit-1 5- treatment1+ foliar application of seaweed extract in concentration of 5 gram.lit-1 6- consumption of Mycorrhiza in the form of hole-layed and 7- combination of treatments 2, 3, 5 and 6. The results of the experiment showed that the effects of irrigation treatment on the characteristics such as number of fruits per tree, acidity of fruit juice and all nutrients, except nitrogen, were significant. Also, the effects of growth biostimulants on all the studied characteristics such as yield and number of fruits per tree, acidity of fruit juice, percentage of soluble solids and concentration of all macro and micro elements were significant. The highest fruit yield.tree-1, hectare-1 and the number of fruits.tree-1 were obtained using seaweed and 70% irrigation in the first year of the experiment with 29.2 kg and 29200 kg.ha-1 and 96.66, respectively.In total, the results showed that reducing the amount of irrigation water calculated based on the water requirement of pomegranate up to 30%, did not have a significant effect on fruit yield and some characteristics of pomegranate. The consumption of biostimulants influenced the yield and some characteristics of pomegranate.

It is very important to find ways to increase the tolerance of plants under drought stress conditions. In order to study the effect of drought stress levels and types of humic fertilizers, amino acid and organic supplements on quantitative and qualitative traits of safflower (Goldasht cultivar), an experiment was conducted as split plots base on randomized complete block design with three replications at the research farm of Zabol University (Chah Nimeh). The main factor of irrigation cut-off levels based on plant growth stages: control (full irrigation), irrigation up to the main stem formation stage and irrigation up to the stage of flowering completion and the secondary factor of five levels of fertilizer treatment: Control (not applicable), amino micropower fertilizer, humic acid, humica and organic supplements in amounts of two per thousand. Maximum plant height (69.76 cm), number of leaves per plant (78.43), number of branches (6.2), oil content (34.2), chlorophyll a, b and carotenoids (11.51, 3.37, 2.40 mg/g fresh weight respectively) was obtained from foliar application treatment with humic acid and complete irrigation. Percentage of changes in plant height (27.7), number of leaves (19.5), number of branches (35.5), petal weight (50), oil content (48), chlorophyll a, b, carotenoids (respectively 33, 38 and 27) under complete irrigation and foliar application of humic acid due to severe stress and non-application of fertilizers. Catalase enzyme activity (97%), peroxidase (99%) and Protein content (62%) increased due to stress and foliar application of humic acid. In general, it seems that foliar application of humic acid compared to other organic fertilizers used in this study can achieve better results under drought stress conditions.

The use of chemical compounds to increase and maintain vegetative and reproductive growth in horticultural products has become common. Humic acid is an organic substance that stimulates growth due to the release of nutrients. In this study, the effect of humic acid with concentrations of 50 and 100 mg/L in two stages 30 and 45 days after flowering, were sprayed on pistachio trees of Akbari cultivar. Then, characteristics such as leaf area, chlorophyll index, branch length and diameter of the current year, percentage of hollow, split rate, percentage of deformed fruits, fruit weight, cluster weight and number of fruits per cluster, and yield were studied. The results showed that the highest leaf area, length and branch diameter were related to pistachio trees with 100 mg /L humic acid in 30 days after full flowering stage. The lowest percentage of hollow was observed in fruits treated with humic acid 50 mg /L. The highest cluster weight and number of fruits per cluster was observed in trees treated with humic acid 100 mg /L. The highest cluster weight and number of fruits per cluster was observed in trees treated with humic acid 100 mg /L. Increased yield and fruit weight in both treatments were observed with increasing humic acid concentration up to 100 mg/L. The percentage of deformed fruits in control fruits increased in both stages of foliar application compared to fruits treated with humic acid.

In recent years, along with the need to use more green space and ornamental plants, cut flowers have also become extremely important. In our country, with the variety of suitable climate, bright sun and genetic resources, many flowers were obtained due to the increasing importance of flowers and ornamental plants. Job creation and currency exchange can have a good position in the big trade market. Carnation is one of the most important cut flowers in the world, which is widely used for decoration and flower arrangement. So that this flower is considered as the bestselling cut flower in the world. In this study, in order to investigate the effect of organic fertilizers on cloves and compare them with experimental chemical fertilizers using 4 types of fertilizers, namely humic acid, folic acid, urea and NPK, respectively, by foliar application at 5 levels (zero, 3 ml of humic acid). 0.4 g of folic acid, 0.5 g of urea, 1.25 g of complete fertilizer). The experiment was conducted in a completely randomized block design in the greenhouse. The measured traits were total chlorophyll content and soluble sugar in leaves. The results of this study indicated that the use of leaf humic acid and folic acid increases the amount of chlorophyll and sugar dissolved in Carnation and is a good alternative to chemical fertilizers.

To investigate the effects of chemical, organic and biological fertilizers application on the quantitative and qualitative characteristics of seedless barberry (Berberis vulgaris cv. Asperma) an experiment was conducted as split plot randomized complete block design with three replications in commercial garden in Qaen during 2016. Main-treatments comprised four application type of fertilizer (1-no fertilizer application as main control, and application of 2-Chemical fertilizer; 70 kg ha-1 urea, 52.5 kg ha-1 triple super phosphate, 77 kg ha-1 potassium sulfate), 3-organic fertilizer; 3500 kg ha-1 cow dung and 4-50% of chemical along with 50% of organic fertilizer; 1750 kg ha-1 cow dung, 35 kg ha-1 urea, 26.25 kg ha-1 triple super phosphate, 37.5 kg ha-1 potassium sulfate). Sub-treatments were 1-no fertilizer as sub control, application of 2-Phosphate Barvar-2; 700 g ha-1, 3-humic acid; 4 kg ha-1, and Phosphate Barvar-2 + humic acid; 700 g ha-1 + 4 kg ha-1. The main and sub-treatments had significant effect on most traits. The highest vitamin C content, berries pH, and dry yield of berries belonged to 50% application of chemical and organic along with Phosphate Barvar2 + humic acid. The highest amount of chlorophyll a, total chlorophyll and leaf carotenoid, carbohydrate, fruit soluble solids content, fruit maturity index and carbohydrate to total acidity ratio were observed in plants treated with organic fertilizer along with Phosphate Barvare2+humic acid. Plants treated with 50% (manure + chemical fertilizer) along with humic acid had the highest leaf chlorophyll b and berries protein. Chemical fertilizer treatment along with Phosphate Barvar2 had the highest acidity of berries. These findings suggested that plants treated with 50% (organic + chemical fertilizer) along with Phosphate Barvar2+humic acid and organic fertilizer along with Phosphate Barvar2+humic acid had the highest positive effect on yield and berries quality, respectively.

Using of humic acid and adding Biochar to soil could be used as an effective way to reduce the harmful effects of drought stress in Zinnia elegans. The effects of three factors: biochar (three levels 0, 20 and 40 g / kg of potting soil), humic acid (three levels 0, 250 and 500 mg / L, soluble) and two irrigation levels (50 and 100% crop capacity)was investigated in this experiment. According to the results, drought stress reduced the amount of chlorophylls, content phosphorus and potassium leaves, but increased amount carotenoids, ion leakage, proline, nitrogen and magnesium elements. The use of Biochar increases potassium and ion leakage. Humic acid reduced the content of proline in the studied plants, reducing the drought stress. The effect of Biochar and humic acid on the amount chlorophyll a and total chlorophyll, nitrogen and phosphorus were significant. The use of humic acid causes confront with drought stress and its use under drought stress conditions is recommended. The use Biochar is advisable in lack of phosphorus and nitrogen elements.

Iran is one of the arid and semi-arid regions of the world. Crop production at optimal yield levels in calcareous soils and high pH soils has always been problematic. An important part of these problems arises because in these soils, due to their high pH and high concentration of calcium ions, nutrients that are pH-dependent can become insoluble and unusable for plants. Adding these elements to the soil through chemical fertilizers will cause environmental problems and pollution. Therefore, in this study, the effect of sulfur combined with Thiobacillus and humic acid bacteria on soil pH and increase in soil organic matter was achieved. For this purpose, a factorial experiment was conducted in a completely randomized block design with 4 treatments in 3 replications and 2 replicates in each tree. Experimental treatments included garden soil, humic acid of organic origin (coal), and humic acid of plant origin (sugar beet) each at 3500 g / ha and 500 g of thiobacillus with 25 kg of sulfur per hectare. The traits tested were leaf dry weight, leaf area index, leaf number and stem length. Based on the results of this study, all morphological characteristics of magnolia trees were significantly improved compared to calcareous soil. It was also observed that thiobacillus treatment with sulfur was the most effective treatment for magnolia tree growth compared to other treatments

 Salinity is one of the most important ecological stresses which reduces productivity. Humic acid as an organic acid and ascorbate as a strong antioxidant can be effective in improving plant yield under salinity stress.In this study, to investigate the effect of ascorbic acid and humic acid on the amount and chemical constituents of essential oil (Dracocephalum moldavica L.) under salinity stress, a factorial experiment in a completely randomized design with three replications was conducted at research greenhouse of faculty of Agriculture, Ferdowsi University of Mashhad, Iran. Treatments included salinity at four levels (0, 50, 100 and 150 mM), humic acid and ascorbic acid at three levels (0, 100 and 200 mg / l).The aerial parts of plant in blooming were collected and were extracted by steam distillation method using Clevenger apparatus and its components were separated and identified by gas chromatography and gas chromatography coupled to mass spectrometry. The essential oil content decreased with increasing salinity stress and application of salt stress moderators (ascorbic acid and humic acid) improved this trait. So that at 150 mM salinityit reached to the lowest level (0.2%) and application of 200 mg / l humic acid increased 51.61% of essential oil compared to control. Most of the essential oil constituents in control treatment (no use of moderators and no salinity conditions) were geranial (36.65%), neral (31.94%), geraniol (15.56%), geranyl acetate (0.66), Trans- 4,2- heptadienal (1/25%), linalool (1.09%), pulegone (0.95%) and verbenol (0.4%) which included 99.5% of the essential oil components respectively by 36.65, 31.94, 15.56, 11.66, 1.25, 1.09, 0.95 and 0.4 percent. In different levels of salinity stress with 100 mg/l of humic acid treatment and application of salt stress moderators (100 and 200 mg/l ascorbic acid and 200 mg/l of humic acid) a large number of combinations was observed compared to control treatment. These includedalpha-pinene, beta-pinene, camphene, camphor, alpha-thujone, nerol and thymol.

In order to investigate the effects of humic acid and application of Tiobacillus fertilizer on better absorption of elements from limy soil and growth and development in Magnolia tree, a factorial experiment was conducted in a randomized complete block design with 4 treatments, each with 3 replications. Treatments included Loamy soil, organic humic acid (coal) and humic acid of vegetable origin (sugar beet) and Tiobacillus fertilizer with sulfur. The traits tested included Index of cell membrance stability and Relative water content of leaves. According to the results of this study, with the application of humic acid and Thiobacillus treatment with sulfur, the relative permeability of the membrane decreased and the relative humidity of the leaf of the tree increased. It was also observed that Thiobacillus treatment with sulfur was the most effective treatment in the desired traits and therefore in calcareous soils the application of Thiobacillus with sulfur powder could be recommended for cultivation and maintenance of Magnolia.

In order to evaluate adjusting salinity by using humic acid and ascorbic acid in Moldavian balm, factorial experiment in a completely randomized design with three replications was conducted. Experimental treatments included salinity at four levels (0, 50, 100 and 150 mM), humic acid and ascorbic acid in three levels (0, 100 and 200 mg/l). Growth characteristics of plant such as subshrub length, inflorence length and root traits showed a significant decrease compared to control by increasing salinity stress and application of humic acid and ascorbic acid at both levels (mainly levels of 200 mg/l) have been improved these characteristics in comparison with control plants. Relative water content also decreased by salinity stress and the application of 100 and 200 mg/l of humic acid and ascorbic acid (to some extent) could compensation this damage. Also Electrical conductivity increased significantly under salinity stress and the usages of humic acid improve this trait. Antioxidant activity and total phenol decreased in 50 mM stress and by increasing salinity levels to 100 and 150 mM increased them. Prolin in different stress levels (50, 100 and 150 mM) was more than control (no salinity) and application of humic acid and high levels of ascorbic acid (200 mg/l) was reduced proline. This result could indicate moderating effect of the mitigation on physiological processes and growth characteristics of Moldavian balm plant under salinity stress, especially in high levels of their usage (200 mg/l).

Salinity is one of the most important non- biological stresses which limit the production of agricultural yields in dry and semi arid regions. Humic acid as an organic acid and ascorbate as a powerful antioxidant can improve yield of plants under salt stress. In order to investigation effects of salinity and its interaction with ascorbate and humic acid on growth, photosynthetic pigments content, antioxidant enzymes activity and soluble protein in Moldavian balm (Dracocephalum moldavica L.), a factorial experiment was conducted based on randomized complete design with three replications. Treatments included four levels of salinity (0, 50, 100 and 150 mM), three levels of ascorbate and humic acid (0, 100 and 200 mgL-1). Vegetative traits such as plant height and fresh and dry weight of stems and leaves with increasing salinity concentration showed a significant decrease compared to control. Humic acid application especially 200 mg.L-1 was improved traits than the control plants in high levels of salinity stress. Photosynthetic pigments were strongly decreased by salinity and application of 200 mgL-1 humic acid and ascorbic acid (partly) could compensate effects of salinity. The highest activity of antioxidant enzymes was observed in 50 mM salinity stress. Application of humic acid (especially 200 mgL-1) for catalase and ascorbate peroxidase and 200 mgL-1 ascorbic acid for guaiacol peroxidase increased enzymes activity compared to the control treatment.

Balanced nutrition nutrients and organic matter of the most important factors affecting the yield and quality of agricultural products. Humic acid as an organic acid derived from humus and other natural resources, with no environmental impact as well as a rich source of nitrogen urea fertilizers are used in agriculture. Traits in the study were: Vitamin C, N, Fe, Zn, Cu and Superoxid dismutase activity. The results showed that the treatment of humic acid 1% with urea and sulphur coated urea have a best result in N, Fe and Zn and humic acid have a best result in Vitamin C, Cu and Superoxid dismutase activity.

Salinity as one of the environmental stresses, limits the growth and performance of plants. Plants hurt with exposing to the salty environment by negative osmotic potential of soil solution and aggregation of toxic ions such as sodium and chlorine. So, the need to select resistant plants to salinity has increased because of shortage in sweet waters and increasing in salinity of soils. In this study, the effect of humic acid application in reducing salinity stress in different varieties of radish was investigated. For this purpose, a pot experiment as factorial based on completely randomized design in three replications was carried out in experimental greenhouse of Ferdowsi University of Mashhad. Treatments consisted of three varieties of radish (Cherry belle, Sparkler and Scarlett cincinnati) as the first factor and humic acid in 2 levels of 0 and 1.5 grams per liter as the second factor and salinity stress at four levels of 0, 33, 66 and 99 mM NaCl solved in water as third factor. The results showed that in some physiological characteristics such as leaf solution sugar and potassium to sodium ratio in root were decreased in salinity stress. Humic acid reduced many harmful effects of salinity, particularly caused to significant reduction in sodium levels in different varieties of radish. Humic acid caused to increase electrolyte leakage, total chlorophyll and the ratio of potassium to sodium in aerial parts and root of radish. The highest and lowest amounts of electrolyte leakage obtained at 99 mM and 33 mM salt level without using humic acid, respectively. The highest (12 mg/g) and the lowest (8 mg/g) amounts of total chlorophyll were obtained in 33 mM and 99 mM salinity levels without using humic acid, respectively. The highest (110 micrograms) and lowest (50 micrograms) amounts of solution sugar were observed in 33 mM salinity without using f humic acid and 99 mM salinity with humic acid application, respectively. Also the highest amount of rootsʼ anthocyanin at salinity level of 66 mM without using humic acid and the lowest amount in this salinity level with humic acid application was measured 3.5 and 1.8 mmol/fresh weight, respectively. Maximum rate of potassium of aerial parts and root were obtained in control level of salinity with 240 and 260 mmol kg-1, respectively. Moreover, the highest (4000 mmol kg-1) and the lowest (800 mmol kg-1) amounts of sodium in aerial parts were obtained in 66 mM salinity without the humic acid application and 99 mM salinity with humic acid application, respectively. The sodium content of root was much lower than aerial parts and its highest amount ​​was observed in 99 mM salinity without using humic acid with 670 mM per gram. Among the radishes varieties, the most effect of salinity and humic acid was observed on Cincinnati scarlet cultivar as that was allocated the maximum amount of many measured traits to it.

The intention of this research is to check the ability of sedum Phytoremediation under the influence of lead and cadmium in a completely randomized design with three replications Treatments Included control Cadmium chloride 5 mg per liter¡Cadmium chloride 10 mg per liter¡ lead nitrate150 mg per liter¡ lead nitrate 300 mg per liter¡ cadmium chloride 5 mg per liter lead nitrate 150 mg per liter¡ cadmium chloride 5 mg per liter lead nitrate 300 mg per liter of¡ Cadmium chloride 10 mg per liter lead nitrate 150 mg per liter¡ Cadmium chloride 10 mg per liter lead nitrate 300 mg per liter¡ Humic acid 100 mg per liter Humic acid 200 mg per liter¡ Cadmium chloride 5 mg per liter Humic acid 100 mg per liter¡ Cadmium chloride 5 mg per liter Humic acid 200 mg per liter¡ Cadmium chloride 10 mg per liter Humic acid 100 mg per liter¡ Cadmium chloride 10 mg per liter Humic acid 200 mg per liter¡ lead nitrate 150 mg per liter Humic acid 100 mg per liter¡ lead nitrate 150 mg per liter Humic acid 200 mg per liter¡ lead nitrate 300 mg per liter Humic acid 100 mg per liter and lead nitrate 300 mg per liter Humic acid 200 mg per liter. Attributes evaluated as follows: Shoot dry weight; Shoot fresh weight; Full leaf chlorophyll¡ the amount of proline in Shoot; the amount of lead in Shoot¡ the amount of cadmium in Shoot and shoot activity of the enzyme superoxide dismutase. The results showed that humic acid treatment of 200 mg per liter most favorable effect on dry weight and fresh weight of the shoot¡ full leaf chlorophyll and shoot activity of the enzyme superoxide dismutase. Also the lowest level of proline and Accumulation of lead and cadmium has been observed in this treatment. Combined treatment of cadmium chloride10 mg per liter lead nitrate300 mg per liter had the most depressing effect upon Treated plants and the highest level of proline and Accumulation of lead and cadmium has been observed in this treatment.