جستجوی مقالات مرتبط با کلیدواژه « لاکتوباسیلوس پلانتاروم » در نشریات گروه « زیست شناسی »

Wound healing and skin remodeling occur directly after skin damage, so the use of platelet rich growth factors (PRGF) and probiotics is important to accelerate this process because of their positive effects on wound healing and antibacterial activities. Combination of above biomaterials with tissue engineering techniques led to the production of a new wound dressing. Therefore, in this study, PRGF was obtained from platelet-rich plasma and a multi-layered scaffold was fabricated by electerospining method using polyurethane (PU) fibers, PRGF and gelatin fibers. Scanning electron microscopy (SEM), tensile and water contact angle tests were performed to assess the characteristics of the scaffolds. The human Adipose Mesenchymal Stem Cells (hAMSCs) were extracted and cultured with the fibroblast cells (HU-02) as co-culture cells and Lactobacillus plantarum was cultured on scaffolds with or without PRGF to evaluate cell viability, toxicity and proliferation, then antibacterial activities of L.plantarum were examined. The result of MTT assay after 14 days indicated that PRFG and L.plantarum had significant positive effect on viability and proliferation of co-culture cells. SEM photograph illustrated adhesion and proliferation of cells and bacteria on scaffolds up to 21 days. The Agar-well diffusion test confirmed the antibacterial effect of L.plantarum on Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli with strong inhibition zone. The current multi-layered scaffold provides the appropriate wound dressing for cell adhesion, proliferation and prevents wound infection.

The present study aimed to examine the effects of Lactobacillus acidophilus and Lactobacillus plantarum probiotics separately and synergistically on the immune system, growth factors, and disease resistance in the rainbow trout.

At in vitro level, bacteriocin extracts of the target bacteria were used in the antimicrobial test against the fish pathogen Aeromonas hydrophila. Next, their effects on the fish blood lymphocytes were evaluated. At in vivo level, after feeding the fish in each treatment with the lactobacilli at the concentration of 108 CFU g-1 for 60 days, the growth indices were calculated. Then, blood samples from the rainbow trout were used to analyze any increase in the number of lymphocytes. Furthermore, given the infectious challenge with the pathogen Chryseobacterium aquaticum, the survival rates of the fish were determined.

L. acidophilus had the best effect on the growth factors and significantly increased the number of fish blood lymphocytes (p <0.05). The broth microdilution assay also was shown to reduce the amount of A. hydrophila colonies by more than 90%. Treatment with this lactobacillus species produced the highest survival rate with this infectious challenge. Then, the synergistic and the L. plantarum treatments were analyzed, respectively, which each showed a significant increase in the indices under study.

According to obtained results, it might be concluded that the feeding by L. acidophilus could likely enhance the immune responses and growth indices in fish both separately and synergistically with L. plantarum.

Natural juices are an excellent substrate for the production of synbiotic functional food for the high nutritional value and common use. Therefore, the survival of the probiotic bacteria and the technological effects of bacteria and inulin were studied on the pomegranate juice quality. Inulin in different percentages (1.5, 3, 5) and Lactobacillus plantarum bacteria equivalent to 0.5 McFarland were added to pomegranate juice. The number of bacteria and the ability of inulin and bacteria in inhibition of fungi and preservation of antioxidant quality of pomegranate juice was considered in compare to the control sample, probiotic sample and prebiotic sample with 3% inulin due to the prohibition of preserver usage in functional food. Based on the results, the pomegranate juice sample containing 3% inulin was able to protect the number of lactobacilli bacteria to 1.5 × 107 cfu/ml in the 4th week in addition to the highest sensory score. Decrease of the antioxidant activity and counting of the fungi were 25.4%, 150 cfu/ml in the control sample, 0.38%, 90 cfu/ml in the prebiotic sample with 3% inulin and 0.26%, 65 cfu/ml in the symbiotic sample with 3% inulin, respectively, in the fourth week. Finally, production of the pomegranate juice with 3% inulin can effect on the maintenance of the antioxidant quality and increase of the product shelf life up to 4 weeks meanwhile making a functional product with preserving of the suitable number of probiotic bacteria.

Biosurfactants are metabolites produced by microorganisms which have potential capabilities in various industries due to abundant beneficial properties. In spite of great advantages, commercial utilization of biosurfactants especially in food industry and pharmaceuticals are limited for the reasons of technical and commercial such as low yield, high production cost, and the type of producing strain. Majority of biosurfactant producer microorganisms have ever evaluated, are pathogenic strains which are not acceptable in industrial and environmental utilization particularly in health and cosmetics, pharmaceuticals and food industries. However, the present study aims to investigate high production of cell-bound biosurfactant by lactic acid bacterium Lactobacillus plantarum through optimization of the main carbon source of specific culture medium. Therefore, three culture media with different amount of glucose were evaluated for biomass and biosurfactant (by surface tension reduction of phosphate buffered saline) production in shake flasks and bioreactor (controlled temperature, pH and agitating speed). The results from both shake flasks fermentation and bioreactor showed the maximum biomass concentration of 3.9 and 4.17 g/L, the minimum surface tension of 41.17 and 40.48 mN/m and subsequently the maximum biosurfactant production in culture medium including 30 g/L of glucose, respectively. Furthermore, fourier transform infrared spectroscopy analysis indicated the biosurfactants are structurally a mixture of protein, polysaccharide and possibly phosphate group, possessing glycoprotein structure.

Growth and immunity resistance of fish are the most important factors in aquaculture. therefore, many types of research in probiotics, prebiotics, and synbiotics for improving growth and immunity in fish have been studied in the last years.

In this study, effects of Lactobacillus plantarum and MOSon growth performance, digestive enzymes activity and intestine morphology of Oncorhynchus mykiss were studied. Juvenile rainbow trout (n=270) in 6 administration  groups in 18  tanks were kept and fed with diets containing different doses (0.3 and 0.5 g kg-1 feed) of the probiotic,  (2 g kg-1 feed) of prebiotic, (0.3+2 and 0.5+ 2g kg-1 feed of probiotic and prebiotic respectively) and  a control group for 45 days.

Results showed a positive effect on growth performance  and digestive enzymes activity in probiotic (0.3 and 0.5 g kg-1 feed of probiotic) and synbiotic groups  compared to the control group(p<0.05).

Probiotics refers to a group of living microorganisms that have beneficial effects on host health. Lactobacilli are one of the most important probiotic bacteria. The stimulatory effects of the immune system, besides reduction of gastrointestinal infections and the risk of inflammation of intestines in humans and animals, are some of Lacobacillus benefits. The effect of rhizospher bacteria, like Lactobacillus, on production of plant hormones, dissolving plant nutrient, facilitates the absorption of nutrients (like nitrogen), and the reduction of plant diseases were reported. Isolation of Lactobacillus has been reported of various sources of dairy and non-dairy sources. The purpose of this study was to investigate the presence of L. plantarum, one of most important probiotic bacteria, in rice rhizosphere, along with examination of the morphological, molecular, biochemical and probiotic properties of the isolated microorganisms. Sampling of rhizosphere rice variety was carried out in Lenjan (Lenjan, Isfahan) carried out. Dillutions of samples were transferred to MES medium. Biochemical test, as sugar fermentation and enzyme activity, performed on gram-positive isolate bacilli. The ability of bacteria to grow in presence of Bile salt, and different pH and temperatures was investigated. Antibiotic resistance of isolates was examined. Specific primers of L. plantarum was used for molecular identification. Of 16 microorganisms, isolated from the rice's rhizosphere, there were 10 bacillus-shaped bacteria, which eight gram-positive isolates were oxidase and catalase negative (characteristic of Lactobacillus species). Sugar fermentation experiments identified six isolates with L. plantarum characteristics. Molecular experiments with the specific primers of L. plantarum confirmed the results. The bacteria grew very well (similar to control samples) in acidic medium at pH 3.5, 40ºC, and in presence of 0.3% bile. Resistance to seven of 12 antibiotics was detected. Discussion and conclusion: The present study showed that rhizosphere of rice is the natural source for isolationof L. plantarum.

Probiotics are live microorganisms, that their usage in host, strengthen and balance the intestinal microflora and have beneficial effects on host health as well. Lactobacillus are the most common type of probiotic microorganisms, which Lactobacillus plantarum is one of the most important of these bacteria. Reduction of intestinal infection and gastro-intestinal inflammatory diseases risk, and immune stimulating efficacy are believed provieded by consumption of Lactobacillus plantarum containing food products. The purpose of this research is identification the probable presence of bacteria Lactobacillus plantarum in apple vinegar using molecular and biochemical methods. Microorganisms were isolated from apple vinegar and some biochemical characteristics such as fermentation of sugars, and extracellular catalase and oxidase enzymes activity, were studied. Molecular identification of strains was performed by specific primer. Their probiotic properties, as ability to grow in different pH and bile salt concentrations, were examined. Of eight microorganisms, isolated from apple vinegar, three strains presented specific band by Lactobacillus plantarum specific primers. These bacteria, were gram-positive and catalase and oxidase-negative. These strains grew pretty well in acidic and bile environments. The results showed that apple vinegar could be a good source for isolation of Lactobacillus plantarum.

Treatment with microorganisms is a new developing method that in this field, probiotic bacteria, especially lactic acid bacteria have a significant role. Olive is a productive and useful Iranian native plant which is rich in lactic acid bacteria. In this study, a number of Lactobacillus plantarum strains were isolated from Iranian native olives and some of their probiotic properties were investigated. At first, different strains of lactic acid bacteria were isolated from Iranian native olive on MRS agar medium, and after biochemical and molecular identification of bacteria, some probiotic properties such as acid and bile salt tolerance; and antimicrobial effects against two pathogenic species of Enterobacteriaceae, Escherichia coli (PTCC1399) and Shigella dysenteriae (PTCC1188) were studied by disk and well diffusion agar methods. According to error reduction, each test was performed in triplicate. Average zone of inhibition for each strain was compared by one-way ANOVA. According to biochemical tests, 57 percent of 28 isolated lactic acid bacteria were identified as Lactobacillus plantarum. Among these strains, 75 percent and 81.5 percent were acid and bile tolerance respectively. In the molecular analysis, 6 strains were shown specific bands in PCR reaction which performed by specific primers designed for Lactobacillus plantarum. The results described antimicrobial effects of Lactobacillus plantarum isolated from Iranian native olives on two enteric pathogens, Escherichia coli and Shigella dysenteriae. Discussion and According to probiotic properties of isolated Lactobacillus plantarum from Iranian native olives, using them is acceptable as an important and practical approach to prevent infectious disease and its treatment due to Escherichia coli and Shigella dysenteriae.

Aim and Biosynthesis of nanoparticles using microorganisms is a noticeable method due to reduced use of energy and expenses as well as its environmental adaptation properties. It attracts many researchers as bio-method-based producing procedures were brought forth for discussion. Use of microorganisms in nanotechnology is one of the most important aspects of the discussion. Native Lactobacillus of Iran derived from some local dairy products was used in this study and exposed to Tio2 solution. Samples were delivered to the relevant labs to perform the experiments 72 hours after incubation. The Titanium nanoparticles were characterized by Scanning Electron Microscopy and Atomic Force Microscopy. Analysis of Titanium Nanoparticles with XRD was prepared. Nanoparticles Formation of Titanium in some Lactobacillus Plantarum strains was observed during the study. After some molecular mechanism deliberations, biosynthesis of nanoparticles using microorganisms and metal reduction via bacteria interpreted as a defensive process may be due to superoxide dismutase lack in some Lactobacillus Plantarum strains.

Aim and Background. Probiotics are microorganisms in the gastrointestinal tract that besides helping the digestion of complex molecules, also helped the immune system and will reduce inflammation. The beneficial effects of these bacteria on ulcer healing has been proved but little research has been don done in the field of healing effects of these bacteria on cotaneous wound healing. Therefore, the purpose of this experiment is study the effect of lactobacillus plantarum isolated from Iranian traditional dairy products on cutaneous wound healing. Materials and Methods. After isolated 22 strains of lactobacillus from Iranian traditional-dairy products and measuring exopolysaccharide production in stains by phenol-sulfuric acid method eventually, Lactobacillus plantarum selected because it has a high exopolysaccharide (EPS) production. Wistar male rats divided into 3 groups; experimental, control and negative control (n=5) and a full-thickness wound (1.5 × 1.5 cm) was made on the back of each rat (45 rats in 3 groups). After 24 hours of injury, Two groups, control and experimental, were treated by eucerin and eucerin contained lactobacillus plantarum, respectively, but the negative-control group did not receive anything. On days 1, 3 and 14 rats were killed and wound samples were collected for histological and statistical studies. Results. Lactobacillus plantarum significantly decreased wound area compared to control and negative control groups and increased wound healing. Histological study on day 3 showed significant increase in number of neutrophils and significant increases in macrophages and fibroblasts (p<0.001). Also significant reduction in neutrophils, macrophages and fibroblasts numbers observed on day 14 (p<0.001). Conclusions. The current study presented a significant decrease in inflammation and an acceleration of wound healing in Lactobacillus plantarum treated rats as compared to the control and negative control groups. Further studies are required to develop a detailed mechanism of Lactobacillus plantarum during cutaneous wound healing.