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  • منصور راسخ*، فریبا علی محمدی سراب، یوسف عباسپور گیلانده، ولی رسولی شربیانی، امیرحسین افکاری سیاح، حامد کرمی

    ذرت (zea mays) یکی از مهم ترین گیاهان زراعی در دنیا محسوب می شود، به گونه ای که بعد از گندم و برنج در رتبه سوم از نظر سطح زیر کشت قرار دارد. هدف از این مطالعه تمایز و طبقه بندی دانه های ذرت در سه رقم بطور غیرمخرب با استفاده از فناوری پردازش تصویر می باشد. سه رقم بذر ذرت در دو حالت تکدانه و توده تحت تصویربرداری قرار گرفتند. از 180 نمونه بصورت تکدانه با 60 تکرار (در حالت پشت و رو)همراه با اندازه گیری وزن و ابعاد دانه ها برای هر رقم، همچنین از 9 نمونه دیگر بصورت توده با 3 تکرار همراه با اندازه گیری وزن و ابعاد ده عدد دانه با انتخاب تصادفی از هر نمونه توده ای برای هر رقم استفاده شد. متغیرهای پیش بینی کننده شامل مساحت، محیط، قطر اصلی بزرگ، قطر اصلی کوچک، یکپارچگی، بی قاعدگی، مساحت محدب ، قطر معادل، شاخص رنگ قرمز ، شاخص رنگ سبز ،شاخص رنگ آبی ، وزن و ابعاد سه گانه اندازه گیری شده بطور دستی در کنار پارامتر جهت تصویربرداری بودند. نتایج نشان داد در طبقه بندی با روش آنالیز تشخیصی خطی با در نظر گرفتن 16 متغیر پیش بینی کننده دقت 70/6 درصد و با روش گام به گام و حذف برخی متغیرها و استفاده از 8 متغیر پیش بینی کننده همان دقت 70/6 درصد بدست آمد. مهم ترین متغیرهای پیش بینی کننده عبارت بودند از: ضخامت، محور اصلی بزرگ، محور اصلی کوچک، بی قاعدگی، قطر معادل، یکپارچگی، شاخص رنگ قرمز و شاخص رنگ سبز. همچنین دقت روش تحلیل شبکه های عصبی مصنوعی (ANN) با 16 متغیر پیش بینی کننده و 8 متغیر پیش بینی کننده به ترتیب برابر با 75/6و 72/2درصد به دست آمد که این مقدار بالاتر از روش LDA بود.

    کلید واژگان: ذرت، طبقه بندی، پردازش تصویر، شبکه های عصبی مصنوعی، LDA
    Mansour Rasekh *, Fariba Alimohammadi Sarab, Yousef Abbaspour-Gilandeh, Vali Rasooli Sharabiani, Amir Hossein Afkari-Sayyah, Hamed Karami
    Introduction

    Maize (Zea mays. L) is one of the most important crops acrossthe world that ranks third in terms of acreage behind wheat and rice. As this crop can adapt to different climatic conditions, it is of great importance and has a large area under cultivation.Therefore, maize is one of the major products of temperate, warm-temperate, subtropical, and humid regions. After wheat, rice, and barley, this plant is the main crop in Iran with the largest cultivated area.There are different types of maizeseeds, so their classification is essential to ensure quality. A key component of sustainable agriculture is quality assurance. On the one hand, techniques such as drying, cooling, and edible coating must be used to maintain the quality of agricultural products. On the other hand, effective and efficient methods should be developed to evaluate and classify their quality, which is used in seed and seedling processing centers, silos, and mechanized warehouses.The detection of various varieties of crop seeds using instrumental methods has been the subject of extensive research. As a non-destructive and rapid inspection method for the recognition and classification of cereal seed varieties, the visual machine is available. Machine vision-based automated methods can have a positive impact on food processing. In other words, this tool is the process of preparing and analyzing images of a real scene using a computer to obtain information or control a process. The features of images can be extracted using this machine to recognize and identify the quality of different types of products. To identify the types of plants, their growth patterns, and the effects of the environment on them to obtain more and superior products, machine vision occupies a special place and is one of the most important research areas. Inspection and quality control of factory output products is an important application of machine vision.Advances in image processing technology have opened up a wide range of machine vision applications in agriculture. The development of powerful microcomputers and specialized software has led to the use of image processing for the inspection of fruits and agricultural products, especially for quality control and sorting. Many agricultural products sorting systems used to separate fruits or crops based on color, shape, size, the extent of damage, crushing, bursting, spotting, etc., now rely on visual machines and image processing functions.Images of products moving on the conveyor system are taken by a CCD camera, transmitted to a computer for processing, and in these systems, the necessary data are extracted from them. Depending on the information obtained, commands are then issued to activate or deactivate a mechanical part so that the product can be removed from or allowed to cross the main path. Sorting is a common practice in many industries. Compared to mechanical systems, machine vision technology offers the highest accuracy and quality at the lowest cost and with the lowest error rate, so it can be considered the most effective solution to this problem. The agricultural industry is one of the areas where sorting and grading systems based on machine vision are urgently needed.The core elements of machine vision are image processing and analysis used together with new methods and classifiers such as neural networks, backup vector machines, fuzzy logic, etc. to perform classifications and required measurements. This study aimed to identify seeds of three maize varieties using macroscopic imaging techniques, evaluate the morphological and chromatic features in maize grains, and discriminate varieties using a stepwise method and remove some variables using LDA and ANN.

    Methodology

    Three seed varieties of single cross 703,single cross 704, and single cross 705 were provided by the Agricultural and Natural Resources Research Centre of Ardabil Province in Pars Abad Moghan. The seeds were then taken to the Biophysical Properties Laboratory of the Department of Biosystems and Mechanical Engineering, MohagheghArdabili University.Three samples (20 g) of each variety were stored in a laboratory oven at 105 °C for 24 h to determine the initial moisture content of maize grains. According to the dry weight of grains, the initial moisture content of them was calculated by 10.50%. To distinguish 3 maize varieties, 180 samples were analyzed as single seeds (30 replicates in the anterior direction and 30 replicates in the posterior direction) for each variety with 60 replicates. In addition, 9 more samples were used in bulk with 3 replicates for each variety.Thus, we imaged a total of 189 samples. In addition, a digital scale with an accuracy of 0.001 g was used to measure the weight of the grains. Computer vision systems consist of five main components: lighting chamber, camera, analogue-digital card (for digitization), computer, and computer software. Images were taken using a Canon IXY DIGITAL 510 IS digital camera. A dome-shaped chamber was used to reduce noise and control ambient light. The system was illuminated with four fluorescent lamps and two rows of LED lamps, one white and one yellow. While the camera was pointed perpendicular to the imaging surface, it provided images with a resolution of 12.1 megapixels.In this case, the images were processed using MATLAB software. First, 10 maize seeds were randomly sampled from the first variety (single cross 703) and weighed using a digital balance. Then, parameters such as the large and small diameters and thickness of each grain were measured using a caliper of 0.02 mm. Then, these grains were placed at appropriate distances from each other on a plate of red cardboard in the opposite direction to be imaged. Finally, 30 maizeseeds were imaged in both directions and 60 images were taken as single seed. In total, we obtained 180 images of all three varieties as single seeds. To prepare the mass, first, some seeds of the first variety were placed in a cylindrical container (1.5 cm high, 4.2 cm in basal diameter, and 70.62174 cm2 in volume) so that the container was filled. After weighing, the mass of grains with a certain volume was poured onto the red plate in a circular pattern. In the end, the camera was placed on the bulk sample and the image was taken, just like the single grain image.The same procedure was repeated twice more on two more bulk samples of the first variety. Similarly, three bulk samples of two more varieties were imaged. In this way, a total of nine images were obtained. After each imaging, we measured and recorded the dimensions and weight of 10 randomly selected seeds from the imaged bulk. In the end, 189 images were obtained, including 180 single-grain and 9 bulk images.In the single sample feature extraction step using the bwlabel function, all samples were labeled and the grain morphological features were extracted. Then, the set of Regionprop functions was used to determine eight parameters, including area, perimeter, major principal axis, minor principal axis, integrity, irregularity, convex area, and equivalent diameter. An artificial neural network (NAA) and a statistical linear discriminant analysis (LDA) method were used to identify maize varieties based on their morphological and color characteristics. The data were normalized before analysis. LDA is a statistical method for classifying objects based on independent variables. The analysis was carried out using SPSS software. The diagnostic analysis includes stepwise analysis, principal component analysis, and elimination of recursive features. In this study, the stepwise method was used. In the usual method, all variables are included in the analysis. However, in the stepwise method, some variables were removed and only the variables with the greatest influence on the model were included. To classify the maize varieties, a network consisting of three layers: input, output, and hidden layers was used.

    Conclusion

    We performed image processing to classify three maize varieties based on the results obtained. A linear diagnostic analysis method was used in this study. A total of 16 predictor variables were used with an accuracy of 70.6%. Some variables were eliminated by a stepwise method. In addition, eight other predictor variables were analyzed with the same accuracy of 70.6%. Thus, although the number of predictor variables was reduced, the detection accuracy remained constant. Moreover, the highest accuracy of diagnosis (80%) was associated with the first variety (single cross 703). Additionally, the accuracy of the methods of ANN with 16 and 8 predictor variables was 75.6% and 72.2%, respectively. These values were higher than that of LDA.Predictive variables included areas, perimeter, major principal diameters, minor principal diameters, irregularities, concave areas, equivalent diameters, color indices (red, green, and blue) resulting from maize grain sample processing, weight, and grain size. The following factors were the most important predictors of varietal discrimination: thickness, major principal axis, minor principal axis, irregularity, equivalent diameter, integrity, red color index, and green color index. According to the results, the length and width of individual grains had no significant effect on variety classification.Our finding demonstrated thatmachine vision technology can be used in seed and seedling processing centers, silos, mechanized warehouses, and other places where maize seed crops need to be identified and separated in a non-destructive manner.

    Keywords: maize, Classification, image processing, Artificial Neural Networks, LDA
  • علی خرمی فر*، اسما کیسالائی، ولی رسولی شربیانی

    در پاسخگویی به یکی از بزرگ ترین چالش های قرن حاضر یعنی برآورد نیاز غذایی جمعیت در حال رشد، تکنولوژی های پیشرفته ای در کشاورزی کاربرد پیدا کرده است. سیب زمینی، یکی از مواد غذایی اصلی در رژیم غذایی مردم جهان بوده و گیاهی است مهم که در سراسر جهان رشد می کند و به عنوان یک محصول مهم در کشورهای در حال توسعه و توسعه یافته برای رژیم غذایی انسان به عنوان یک منبع کربوهیدرات، پروتیین، و ویتامینها به حساب می آید. به دلیل تعدد زیاد واریته های این محصول و برخی مواقع عدم آشنایی واحدهای فرآوری با ارقام آن و نیز وقت گیر بودن و عدم دقت زیاد در شناسایی ارقام مختلف سیب زمینی توسط کارشناسان و زارعین، و اهمیت شناسایی ارقام سیب زمینی و نیز سایر محصولات کشاورزی در هر مرحله از پروسه ی صنایع غذایی، نیاز به روش هایی برای انجام این کار با دقت و سرعت کافی، ضروری می باشد. این مطالعه با هدف استفاده از ماشین بویایی همراه با روش های LDA و شبکه عصبی مصنوعی به عنوان روش سریع و ارزان برای تشخیص ارقام مختلف سیب زمینی انجام شد. بر اساس نتایج به دست آمده برای تشخیص رقم با روش های مذکور دقت روش های LDA و ANN 100 % به دست آمد.

    کلید واژگان: سیب زمینی، LDA، شبکه عصبی مصنوعی، ماشین بویایی
    Ali Khorramifar *, Asma Kisalaei, Vali Rasooli Sharabiani
    Introduction

    Potato is an important vegetable that grows all over the world and is considered an important product in developing and developed countries for the human diet as a source of carbohydrates, proteins, and vitamins. This product is native to South America and its origin is from Peru, after wheat, rice and corn, it is the fourth product in the food basket of human societies. According to the statistics of the Food and Agriculture Organization of the United Nations, the area under cultivation of this crop in Iran in 2017 was 161 thousand hectares and the crop harvested from this area is about 5.1 million tons. Traditional methods of determining potato varieties were based more on morphological features, but with the production of new products, there was a need for methods that were faster and more recognizable. In the meantime, the high-performance artificial neural network can be used to classify cultivars. Artificial neural networks can classify and detect cultivars, are flexible, and are used in most agricultural products. Therefore, the olfactory machine can have high efficiency in classifying and distinguishing cultivar, originality and storage time. The olfactory machine is a system that has a different structure and approach from other methods (image processing, neural network, etc.), is flexible and is used in most agricultural products due to the presence of odour in them.With the rapid and rapid advancement of computer technology and sensor technology, the application of the bionic electronic nose, including a semiconductor gas-sensitive sensor and a pattern recognition system as a means of detection, offers a new method for rapid classification and digit recognition. Give. The electronic nose has also introduced a new method for classifying and detecting rough rice in a non-destructive and fast way.Due to a large number of potato varieties and sometimes the lack of familiarity of processing units with its cultivars and also time-consuming and inaccurate in identifying different potato cultivars by experts and farmers, and the importance of identifying potato cultivars and other agricultural products in At every stage of the food industry process, it is necessary to find ways to do this accurately and quickly enough. The aim of this study was to evaluate the ability and accuracy of the electronic nose with the help of an artificial neural network to detect and differentiate several potato cultivars.

    Methodology

    First, potatoes in 3 different cultivars (Colombo, Milvana and Sante) were prepared from Ardabil Agricultural Research Center and kept at a temperature of 10-4 ° C. One day after the data were collected, data collection began with an olfactory machine. 3-4 potatoes from each cultivar were placed in the sample container for 1 day to saturate the sample container with the smell. Then the sample chamber was connected to the electronic nasal device and data collection was performed. The data were collected by the olfactory machine in such a way that first clean air was passed through the sensor chamber for 100 seconds to clean the sensors from other odours. The odour (gases emitted from the sample) was then pumped out of the sample chamber by the pump for 100 seconds and directed to the sensors. Finally, clean air was injected into the sensor chamber for 100 seconds to prepare it for further data collection. According to these steps, the output voltage of the sensors was changed due to exposure to various gases (potato odour) and their olfactory response was collected by data collection cards, sensor signals were recorded and stored in the USB gate of the computer at 1-second intervals. A fractional method was used to correct the baseline in which noise or possible deviations were eliminated and the sensor responses were normalized and dimensionless. In the next step, linear diagnostic analysis (LDA) and artificial neural networks (ANN) were used to classify the 3 potato cultivars. LDA is a supervised method used to find the most distinctive special vectors, maximizing the ratio of the variance between class and within the class, and being able to classify two or more groups of samples. ANN and pattern recognition were used to find similarities and differences in the classification of potato cultivars. For this, 1 neuron was considered for the input layer, the hidden layer with the optimal number of neurons will be considered and five output neurons with Depending on the number of output classes the target will be considered. In-network training, logarithmic sigmoid transfer function and Lunberg-Marquardt learning method were used. Also, the amount of error was calculated using the mean square error. For learning (70%), testing (15%) and validation (15%) all data were randomly selected. Training data was provided to the network during the training and the network was adjusted according to their error. Validation was used to measure network generalization and completion of training. Data testing had no effect on training and therefore provided an independent measurement of network performance during and after training.

    Conclusion

    LDA and ANN methods were used to detect potato cultivars based on sensor output response. The LDA method can extract multi-sensor information to optimize resolution between classes. Therefore, this method was used to detect 3 potato cultivars based on the output response of the sensors. Detection results of cultivars equal to 100% were obtained (Figure 1). Also, in the ANN method, 8 sensors were considered in the input layer of 8 neurons according to the output data. Also, 3 layers of neurons were considered for the output layer according to the type of cultivars. Therefore, the 3-6-8 topology had the highest accuracy for detecting potato cultivars, so the RMSE value was 0.008 and the R2 value was 99.8. There was also a very high correlation between predicted and measured data (Figure 2). In this study, a portable olfactory machine system with 8 metal oxide sensors was used to investigate the detection of potato cultivars. Chemometrics methods including LDA and ANN were used for qualitative and quantitative analysis of complex data using an electronic sensor array. LDA and ANN were able to accurately identify and classify different potato cultivars with 100% accuracy. The electronic nose has the potential to be used as a fast and non-destructive method to detect different potato cultivars. Using this method in identifying potato cultivars will be very useful for researchers to select and produce pure cultivars and for farmers to produce a uniform and certified crop.

    Keywords: Potato, LDA, Artificial Neural Network, electronic nose
  • علی خرمی فر، منصور راسخ*، حامد کرمی، عارف مردانی کرانی

    در پاسخگویی به یکی از بزرگ ترین چالش های قرن حاضر یعنی برآورد نیاز غذایی جمعیت در حال رشد، تکنولوژی های پیشرفته ای در کشاورزی کاربرد پیدا کرده است. سیب زمینی، یکی از مواد غذایی اصلی در رژیم غذایی مردم جهان است. لذا مطالعه روی جنبه های مختلف آن، از اهمیت زیادی برخوردار است. به دلیل تعدد زیاد واریته های این محصول و برخی مواقع عدم آشنایی احدهای فرآوری با ارقام آن و نیز وقت گیر بودن و عدم دقت زیاد در شناسایی ارقام مختلف سیب زمینی توسط کارشناسان و زارعین، و اهمیت شناسایی ارقام سیب زمینی و نیز سایر محصولات کشاورزی در هر مرحله از پروسه ی صنایع غذایی، نیاز به روش هایی برای انجام این کار با دقت و سرعت کافی، ضروری می باشد. این مطالعه با هدف استفاده از خواص مکانیکی همراه با روش های کمومتریکس از جمله LDA و ANN به عنوان یک روش سریع و ارزان برای تشخیص ارقام مختلف سیب زمینی انجام شد. در پژوهش حاضر ، از دستگاه سنتام موجود در دانشگاه محقق اردبیلی جهت تعیین خواص مکانیکی استفاده شد. بر اساس نتایج به دست آمده برای تشخیص رقم با روش های مذکور دقت روش های LDA و ANN بالای 70 % به دست آمد.

    کلید واژگان: سیب زمینی، چقرمگی، شبکه عصبی مصنوعی، طبقه بندی، LDA
    Ali Khorramifar, Mansour Rasekh *, Hamed Karami, Aref Mardani Korani
    Introduction 

    Potato is an important vegetable that grows all over the world and is considered as an important product in developing and developed countries for human diet as a source of carbohydrates, proteins, and vitamins. This product is native to South America and its origin is from Peru, and after wheat, rice and corn, it is the fourth product in the food basket of human societies. According to the statistics of the Food and Agriculture Organization of the United Nations, the area under cultivation of this crop in Iran in 2017 was 161 thousand hectares and the crop harvested from this area is about 5.1 million tons. Traditional methods of determining potato varieties were based more on morphological features, but with the production of new products, there was a need for methods that were faster and more recognizable. Meanwhile, high-performance artificial neural network can be used to classify cultivars. Artificial neural network can classify and detect cultivars, is flexible and is used in most agricultural products. Azizi conducted a study on 120 potatoes in 10 different cultivars using a visual and image processing machine with a MATLAB R2012 software toolbox to detect texture, shape parameters and potato cultivars. First, potato cultivars were classified using LDA method, which obtained 66.7% accuracy. This method also erred in distinguishing the two cultivars Agria and Savalan and also classified the two cultivars Fontane and Satina in other classes. They also used artificial neural networks to classify potato cultivars, in which the network was 82.41% accurate with one hidden layer and 100% accurate with two hidden layers. In this study, it was found that different types of potatoes can be identified and identified with a very high level of accuracy using the three color characteristics, textural and morphological features extracted by the visual machine and the use of a non-linear classifier artificial neural network. Categorized.By determining and examining the existing relations between the force and the deformation of agricultural products up to the point of surrender, the range of forces harmful to fruit can be determined so that harvesting and transportation machines are designed in such a way that the forces from them do not exceed this range. On the other hand, one of the ways to determine the degree of ripeness of the fruit is to touch and press it with the thumb, which is an experimental way and depends on the skill of the person touching. The mechanical penetration test of the fruit can be an indicator to check the ripeness of the fruit by quantifying this diagnosis and using this diagnosis to determine the optimal harvest time.

    Methodology

    First, 5 different potato cultivars were prepared from Ardabil Agricultural Research Center and kept at a temperature of 4-10 ° C. One day later, 21 samples of each potato cultivar were prepared using a cutting cylinder and then data were collected. To determine the toughness of the samples, the Centam device available in Mohaghegh Ardabili University was used. Each potato cultivar was subjected to compressive force at three levels of loading speed of 10, 40 and 70 mm / min with 7 repetitions. Then the amount of toughness was calculated according to Equation (1). Then linear diagnostic analysis (LDA) and artificial neural networks (ANN) were used to classify potato cultivars. LDA is a supervised method used to find the most distinctive special vectors, maximizing the ratio of variance between class and within the class, and being able to classify two or more groups of samples. ANN and pattern recognition were used to find similarities and differences in the classification of potato cultivars. For this, 1 neuron was considered for the input layer, the hidden layer with the optimal number of neurons will be considered and five output neurons with Depending on the number of output classes the target will be considered. In network training, the logarithmic sigmoid transfer function and Lunberg-Marquardt learning method were used (Figure 4), and the error value was calculated using the mean square error. For learning (70%), testing (15%) and validation (15%) all data were randomly selected. Training data was provided to the network during the training and the network was adjusted according to their error. Validation was used to measure network generalization and completion of training. Data testing had no effect on training and therefore provided an independent measurement of network performance during and after training. All of the calculations and matrix classification were performed using MATLAB R2018a and X 10.4 Unscrambler software.Toughness in 5 different potato cultivars was obtained using Centam machine and Equation 1. The values obtained for the toughness of 5 potato cultivars were analyzed using Mstatc software. The results of analysis of variance were significant for the toughness of 5 different potato cultivars at the level of 1% and its coefficient of variation was 2.28. LDA and ANN methods were used to detect potato cultivars based on the values calculated for toughness. Detection results of cultivars using LDA were equal to 70.48% (Figure 6). Also, the accuracy of ANN method according to the perturbation matrix was equal to 72.4% (Figure 7).

    Conclusion

    In this study, the amount of toughness for 5 different potato cultivars was calculated using Centam machine available in Mohaghegh Ardabili University with the help of Equation 1. Chemometrics methods including LDA and ANN were used for qualitative and quantitative analysis of data to identify and classify potato cultivars. Thus, LDA and ANN were able to identify and accurately classify different potato cultivars with an accuracy of over 70%. The obtained toughness has the ability to be used as a method to distinguish different potato cultivars. The use of this method in identifying potato cultivars will be very useful for factories such as chips factory and processing units, and it is also expected that similar methods related to mechanical properties such as crispness and stiffness with the help of chemometrics methods to optimize production and The processing of agricultural products should be used in the food industry, which has led to more customer friendliness and, in addition, can reduce agricultural waste.

    Keywords: Potato, Toughness, Artificial Neural Network, Classification, LDA
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