chemical composition

Good food supply or quality, stable and reasonable price, is one of the main and important problems in the aquaculture industry. In recent years, much research has been done on the use of different species of algae as a source of protein in the aquatic diet. The present study was performed to evaluate the chemical composition, amino acid and premix fatty acid of the premix of brown macroalgae Padina australis, Sargassum ilicifolium and Stoechospermum marginatum.

In this study, after collecting macroalgae from the tidal areas of Chabahar coast, they were washed and after drying in a ratio of 1: 1: 1, they were combined and turned into powder. Chemical composition was measured by AOAC method, fatty acids by chromatography and amino acids by HPLC.

The results showed that the amount of protein, fat, ash and moisture were 8.43, 1.30, 21.26 and 68.01%, respectively. The main component of the macroalgae premix tested was moisture. The predominant saturated fatty acids in the macroalgae premix were palmitic acid (17.11 ± 0.31%), myristic acid (11.43±0.15%) and stearic acid (6.62±0.32%) were respectively. The ratio between non-essential amino acids to essential amino acids was 1.05 .Among monounsaturated fatty acids, oleic acid (17.21±7.21%) and among long-chain unsaturated fatty acids (PUFA), respectively, arachidonic acid (11.24±7.03%), linoleic Acid (9.12±3.21%) and eicosapentaenoic acid (8.13±0.68%) were predominant. The total essential and non-essential amino acids were 6.78 and 7.15 mg amino acids per gram of sample, respectively. Aspartic acid (1.88±0.05 mg amino acid per gram of sample) and glutamic acid (1.98± 0.05 mg amino acid per gram of sample) were the predominant nonessential amino acids.

In general, the results of this study showed that due to the presence of longchain unsaturated fatty acids and the balance between non-essential and essential amino acids, the use of premix of Padina australis, Sargassum ilicifolium and Stoechospermum marginatum macroalgae is recommended in aquatic nutrition.

Sesbania is a perennial legume that grows mostly in the tropics of Asia, Africa and Australia. This plant tolerates different type of soils, especially saline soils. Due to appropriate protein percentage and relatively low fiber, this plant can be used in ruminant diets. This study was conducted to determine the chemical composition and digestibility of sesbania (Sesbania sesban) using gas production technique and to evaluate the trend of changes in these parameters from the third week to ninth week of growth. The samples from whole plant, leaves and stems were collected separately at third, fifth, seventh and ninth week of planting, to measure chemical composition (dry matter, protein, ash, neutral detergent fiber and acid detergent fiber) and also in vitro digestibility by gas production technique. The results of this experiment showed that the percentage of NDF and ADF increased significantly with the growth in the whole plant, leaves and stems, so that in the whole plant the amount of NDF increased from 35.66% in the third week to 46.08% in ninth week, and ADF ranged from 22.90 in the third week to 35.43 in the ninth week. Protein concentration decreased with aging in all parts of the plant. The amount of protein in the ninth week of growth was approximately 17.38% and 21.01% in the whole plant and leaves, respectively. With increasing plant age, along with increasing the concentration of structural carbohydrates and protein reduction in all three samples (whole plant, leaves and stems), the production of cumulative gas after 48 hours, total gas production, metabolisable energy, short chain fatty acids and organic matter digestibility decreased. The results of this study showed that according to the amount of dry matter and other measured compounds, the most appropriate time to harvest this plant as a whole plant is the seventh week of cultivation and the whole sesbania or its leaves can be a good source of livestock fodder after about two months of cultivation.

Digestibility of feeds can be measured using in vivo (direct method) and in vitro methods. The aim of this study was to measured chemical composition and in vitro digestibility of dried pomegranate seed pulp and pomegranate by-products silage by gas production technique. This research was conducted in the Department of Animal Sciences, Shiraz University. The experimental diets include dried pomegranate seed pulp (DPS), pomegranate seed pulp silage (fresh pomegranate seed pulp with 2% urea; FPS), pomegranate pulp silage (mixture of same amount of peel and seed pulp with 2% urea and 3% wheat straw; PPS+U), and the same silage mixture without urea (PPS). The chemical composition (protein, fat, neutral detergent fiber (NDF), and acid detergent fiber (ADF)) and volume of gas production were measured. The results showed the highest percentage of protein (23.33) and fat (16.75) were found in PPS+U and FPS, respectively. The dried pomegranate seed pulp had the maximum amount of NDF and ADF. The maximum gas production volume during 24 hours was found in dried pomegranate seed pulp. The constant gas production rate per hour (c) was not significantly different between the treatments, but other fermentation parameters were higher in DPS than other samples. In general, our results showed that DSP, despite higher amount of NDF, has higher gas production and more energy, and also the PPS+U, is an appropriated protein feed, that these two pomegranate by-products can be tested in in vivo studies by replacement for some part of the forage and concentrate in ruminant rations.

A study was conducted to evaluate the effect priming and inoculation with bio-fertilizers on the chemical composition and in vitro fermentation parameters of lentil. The lentil seeds to priming treatments, with two percent sodium hypochlorite disinfection solution, and cultured for 8 hours before the desired solutions (hormone gibberellic acid, and salicylic acid and distilled water) were kept. Treatments were: The first factor is the use of mycorrhizae in three levels [Non-inoculated (control), inoculation with mycorrhizal fungi species Glomus intraradices and G. mosseae]. The second factor involves priming treatments in 5 levels [Hydro-priming (using water), priming with gibberellic acid 100 ppm, Priming with salicylic acid 100 ppm, Priming with gibberellic acid 100 ppm × priming with salicylic acid 100 ppm and control (no treatment)]. The chemical composition of the samples was determined using the standard methods. In vitro digestibility of samples was determined by the batch culture method. Results showed that there was significant difference among treatments on chemical composition. In the absence of fungus, treatment combination of two hormones had most and salicylic acid and gibberellic acid had lowest crude protein content. In this study, the lowest neutral detergent fiber and acid detergent fiber content was related to treatment Glomus intraradices Glomus intraradices × salicylic acid. Results showed that pre-treatment of lentil seed with salicylic acid and combination of two hormones in drought conditions, compared with Glomus intraradices and Glomus mosseae, increased crude protein, neutral detergent fiber and nutrients digestibility. The highest apgas production potential (294.9 ml) and metabolizable energy (13.48 Mj/kg) were related to treatments Glomus mosseae and Glomus mosseae × gibberellic acid, respectively. Totally, the obtained results of this study showed that any treatment of crop seeds for resistance to diseases, pests and stresses such as salinity and drought, making ability of roots in the absorption of water and nutrients can affect nutritive values of their forages and other by- products.

A study was conducted to evaluate the effect of addition alcoholic extract of propolis and organic acid (acetic acid) on chemical composition, fermentation characteristic and gas production parameters of barley silage in a complete randomized design. Whole crop barley was harvested and chopped with a conventional forage harvester under farm condition to length of 2-3 cm. Representative of barley forage samples were packed manually, in triplicate into plastic bags and were stored at ambient temperature and allowed to ensile for 1, 3, 7, 21 and 45 days. The following treatments were applied to the forage samples: 1) barley silage without any additives (control), 2) control + alcoholic extract of propolis (500 µl/kg of fresh forage) and 3) control + acetic acid (1% of DM).Results showed that there were differences among treatments on DM content and pH (p < 0.05). The highest and lowest pH was related to barley silage treated with organic acid and control treatment, respectively. Compared with control treatment, barley silage treated with acetic acid and alcoholic extract of propolis improved (40 and 18 h, respectively) aerobic stability (p < 0.05). The fungi count was lowest in organic acid treatment on days 3 and 7 (p < 0.05). However, the fungi population was not observed on days 21 and 45 after ensiling in all treatments. Organic acid treated barley silage had highest gas production potential and in contrast, using alcoholic extract of propolis increased ME, OMD and SCFA content. Generally, obtained results showed that although organic acid and alcoholic extract of propolis as additives had not considerably effect on nutritive value of barley silage, but they improved aerobic stability (p < 0.05).

This study was conducted to investigate chemical composition, gas production parameters and digestibility of dominant species of weed plants on in vitro conditions. In this experiment, samples of Echinochola crus-gali, Portulaca oleracea, Sorghum halepense, Amaranthus retroflexus, Chenopodium album and Cynodon dactylon were collected from the Gonbad Kavoos area before seeding. The mean annual rainfall amount is below 450 mm and means annual temperature is above 20 °C. Plant specimens were dried in an oven at 60 °C until they reached constant weight and milled to pass a 1 and 1.5 mm screen. Their nutritive value was evaluated through determination of chemical compositions and in vitro gas production techniques. Samples were tested in an in vitro gas production method (96 h incubation) and batch rumen culture system (24 h incubation). Results showed that there were differences among treatments on chemical composition (P<0.05). Portulaca oleracea had highest Ash and crude protein and lowest NDF and total phenol. Highest content of NDF and total phenol and lowest Ash was related to Sorghum halepense. There were significantly differences among treatments on gas production parameters and Portulaca oleracea and Chenopodium album had lowest lowest and highest gas productionpotential, respectively. Highest and lowest short chain fatty acid content and OMD were related to Echinochloa crus-galli and Chenopodium album, respectively. Portulaca oleraceaand Sorghum halepense had highest and lowest in vitro DMD and OMD, microbial crude protein and microbial crude protein efficiency, respectively. The obtained results of this study showed that different weed plants have different nutritive value; however, these plants can be regarded as feed resources for animal nutrition. In general, the results showed that the weeds in the fields are nutritionally different, which is due to differences in the chemical composition and their nature.

The aim of this study was to determine of nutritive value of green and black tea waste, and determination of metabolizable energy (ME) and net energy for lactation (NEL) using in vitro gas production method. The obtained data was completely randomized design was analyzed using SAS procedures. The values of crude protein, crude ash, ether extract, neutral detergent fiber and acid detergent fiber in green tea waste were 13.7, 3.7, 2, 44.6 and 31.7 %DM, also for black tea waste were 14.8, 4.7, 2.4, 39.6 and 27.1 %DM, respectively. The value of total phenol and extractable tannin for green tea waste were 20.3 and 18.7 %DM, and for black tea waste were 18.7 and 12.7 %DM, respectively. After 96 h of incubation, black tea waste and alfalfa had the highest and lowest gas production volume among the test feeds (p<0.05). The gas production potential amounts of the green and black tea waste and alfalfa hay were obtained 284.2, 294 and 261.9 ml/g DM, respectively. Rate of gas production for green and black tea waste and alfalfa hay were 0.074, 0.074 and 0.072 (h-1), respectively. Black tea waste had the greatest estimated metabolizable energy and net energy for lactation (9.45 and 5.77 MJ/Kg DM) among feedstuffs. These results indicated that black and green tea waste had a nutritive value higher than last bloom alfalfa and can be used as a part of ruminants ration.

This Experiment was carried out to determine the effects of urea and molasses -treated Corn Single Crosses SC 704 Stalklage in fattening performance of Turky-Ghashghaii male lamb. At first, corn residues were gatherd after harvesting of corn grain by Cambine method and processed with urea and molasses. Then, evaluates its chemical composition and in vivo apparent digestibility. The Corn Stalklage treated and untreated were included in the ration as to replace zero , 30 and 50% of total forage in a completely randomized design in the ration of fattening lambs. The rations (iso-nitrogenous and iso-energetic) were fed to 6 Turky-Ghashghaii ram lambs. Results showed that In vivo digestibility coefficients for DM OM, NDF and ADF had significant differences between treated and non-treated Corn Stalklage.  It seems that according to the results of fattening traits and digestibility, the use of treated corn residues with 4% urea plus 10% molasses with ratios of 50% as total forages in feeding lambs in comparison with other treatments, had a better daily weight gain.

According to the importance of prebiotic inulin on sexual maturation in fish, this study changing the chemical composition (fat, protein, moisture and ash) in various stages of maturation muscle tissue in the zebra fish. Therefore, zebrafish larvae were fed with experimental diets containing (0, 1, 2 and 3 gr/kg diet) of inulin and two-stage sampling (45 days and 90 days after the active feeding) took place at every stage in the chemical composition (lipid, protein, moisture and ash) muscle tissue (based on the percentage dry weight) in fish muscle were measured. In the first sampling stage, fat and muscle protein was significantly higher than the second sampling stage (P<0.05). Given that the second stage fish were full sexual maturity, the amount of fat in muscle decreased (P<0.05). Moisture and ash levels were significantly (P<0.05) higher in the second stage sampling than the first stage. Overall, our results showed that different stage of living affected on muscle chemical composition muscle in Zebrafish.