بیوفلوتاسیون

Tabas coal possesses favorable plastometric properties that make it suitable for use in metallurgical industries as coking coal. However, its high sulfur content, which stands at approximately 2%, poses a significant environmental pollution risk. Additionally, reducing ash content to below 10% is a critical objective of this study to prevent a decline in coal's thermal efficiency in the metallurgical industries. This research work investigates the removal of sulfur and ash from Tabas coal samples using the biological methods including bioflotation and bioleaching. Initially, a combination of mesophilic bacteria including Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptosprillium ferrooxidans were employed in the bioflotation method to detain pyrite sulfur in the Tabas coal samples. The highest reduction percentages of pyrite sulfur and ash were equal to 62% and 54.18%, respectively. In the next stage, bioleaching experiments were conducted, the effect of the test time, percentage of bacteria by volume, percentage of coal solids, and absence of bacteria on the amount of sulfur and ash removal was investigated. The test time emerged as the most critical factor. The best sulfur removal was achieved using bioleaching, with a maximum removal of 72.43%, observed for the PE coal sample. Bioflotation also achieved significant sulfur removal, with a maximum removal of 61% observed for the same sample. On the other hand, the best ash removal was achieved using bioflotation, with a maximum removal of 68.98% observed for the PE coal sample, and a maximum removal of 69.34% observed for the B4B2 coal sample using bioleaching. Finally, this research work conducted a comparison of biological methods to determine the amount of sulfur and ash reduction achieved. The results showed that both bioleaching and bioflotation were effective for coal desulfurization and ash removal, with bioleaching performing slightly better for sulfur removal and bioflotation performing slightly better for ash removal.

Flotation is the most important method for processing sulfide copper ores. Due to the high cost and environmental hazards caused by the chemical reagents used in this process (collectors, frothers, pH regulators, depressants, etc.), the possibility of replacing all these reagents or at least some of them are of special importance through environmentally friendly methods such as bio-flotation using halophilic bacteria. These bacteria have the ability of growth and proliferation in salty media and relatively neutral pHs such as sea salty water. In this research work, the four types of halophilic bacteria Halobacillus sp., Alkalibacillus almallahensis, Marinobacter sp., and Alkalibacillus sp. are studied to replace frothers (MIBC and F7240), depressant (sodium metabisulfite), and pH regulator (lime) in sulfide copper flotation using a Denver laboratory flotation cell. The results obtained indicate that each of the four types of bacteria mentioned above along with collectors (gasoil, Z11, and C7240) as the only chemical reagents (bio-flotation + collector) can depress pyrite better than the bacteria-free mode (flotation + all chemical reagents). Iron recovery in tailings in the standard flotation test is 46.8%, which is, respectively, increased to 91.9%, 74.5%, 70.3%, and 76.9% using the halophilic bacteria of Halobacillus sp., Alkalibacillus almallahensis, Marinobacter sp., and Alkalibacillus sp. On the other hand, the recovery of chalcopyrite using the bio-flotation method is lower than its recovery using the flotation method. Copper recovery in the concentrate in the standard flotation test is 89.1%, which is reached to 58.8%, 71.4%, 62.5%, and 69.4%, respectively, using the above bacteria in the bio-flotation method.

Due to the increasing consumption of lime in the flotation process to increase the pH of the system and create an alkaline environment, as well as its gradual increase in cost, the attention of researchers has been drawn to perform flotation operations in a neutral environment. Halophilic bacteria have the potential to replace flotation reducers such as lime because flotation can be done with their help at neutral pH as well. Also, due to the buffer effect of sea water, which is the chosen medium for bio-flotation, the use of bio-flotation method reduces the use of drinking water, and also reduces the consumption of chemicals. In this research work, five types of halophilic bacteria are studied for pyrite bio-depression and chalcopyrite flotation. Bio-flotation experiments are conducted using Hallimond tubes, and the bacteria Halobacillus sp., Alkalibacillus almallahensis, and Alkalibacillus sp. had better performance in pyrite depression and chalcopyrite flotation than other bacteria. The recovery of pyrite depression when using them was 30.9, 30.3, and 34.0 %, respectively, and the recovery of chalcopyrite flotation by them was equal to 52.9, 68.6, and 55.7, respectively, which indicates the high selectivity of these bacteria in flotation. In addition to the above tests, the effect of the combination of these three types of bacteria on pyrite depression and chalcopyrite flotation was also studied. The results obtained indicate that in the combination (mix) test of all three types of bacteria (33.3% of each type), pyrite was depressed better than other tests, and its recovery was 27.5%, which was lower than the single bacteria tests. Also, the effect of the combination of these three types of bacteria on the flotation of chalcopyrite is investigated, and its recovery was 72.6%, which was higher than the single bacteria tests. On the other hand, considering that the recovery of chalcopyrite in the three-bacteria combination tests was is higher than the single-bacteria and two-bacteria tests, it can be concluded that the combination of all three bacteria can cause a better synergism and improve their performance in micro-flotation tests.