جستجوی مقالات مرتبط با کلیدواژه « میکوریزا » در نشریات گروه « آب و خاک »

In order to investigate the effect of different levels of biochar and biological fertilizer (Arbuscular mycorrhizal and Azospirillum lipoferum) on agricultural properties and quality of chamomile essential oil, a factorial experiment based on randomized complete block design with 12 treatments and three repetitions was conducted in Beiranshahr region of Khorramabad city in 2019-2020 cropping year. The factors included: application of biochar at three levels (0, 10 and 20 tons per hectare) and inoculation of biological fertilizers at four levels (without inoculation (control), inoculation with arbuscular mycorrhiza, inoculation with Azospirillum lipoferum and double inoculation of arbuscular mycorrhiza and Azospirillum lipoferum). The results showed that the interaction effect of biochar and biological fertilizers on the number of branches per plant, flower diameter, number of flowers per plant, biomass yield, economic yield (fresh flower), carotenoid content and essential oil yield at the probability level of one percent and on plant height and essential oil percent it was significant at the five percent probability level. Combined use of biochar and biofertilizers in the treatment of using 20 ton.ha-1 of biochar and double inoculation of mycorrhizae and Azospirillum lipoferum compared to other fertilizer treatments, has the highest values of biological yield (174.3 g plant-1), economic yield (3 56.5 g plant-1), essential oil percentage (0.440 %), and essential oil yield (24.6 g plant-1). Also, the use of 20 ton ha-1 of biochar showed more total flavonoids (0.43%), and carotenoids (1.56 mg/g) than other treatments. The highest percentage of camazolin (0.09%) was observed in the double inoculation treatment of mycorrhiza and Azospirillum lipoferum. The results showed that the use of biochar as an organic fertilizer of natural origin along with the inoculation of biological fertilizers increases the quality and quantity of chamomile medicinal plant products in the field and also maintains the balance of the environment.

Current study was carried out by the aim of investigating the effect of rhizobium bacteria and arbuscular mycorrhizafungi on yield and micronutrients concentration on bean under different irrigation regimes in 2016 and 2017 years.In a split plots design, thethree regimes irrigation, S1= 95-100% available water(AW), S2= 75-80% AW and S3= 55-60 % AW were assigned to main plots and six bio-fertilizers, including T1= Rhizobium leguminosarum bv.  Strain of 177 and T2= Rhizobium leguminosarum bv.strain of 160 used for seed inoculation, T3= Arboscular mycorrizal (Funneliformis mosseaeand Rhizophagus irregularis) used for soil inoculation, T4= T1+T2, T5= T1+T2+T3 and T6= control were randomized to subplots. There were significant differences (p˂0.05) in yield and micronutrients concentration under irrigation regimes and bio fertilizers treatments. Highest grain yield (2371 kgha-1) was obtained in irrigation on 75-80 % available water and 160 Rhizobium inoculated treatment. The highest concentration of Fe, Mn, Zn and Cu in seed plant were in irrigation on 75-80% available water and co-inoculated treatment. The addition of Fe, Mn, Zn and Cu concentrations were, 21.4 %, 5.7%, 3.5% and 3.3% respectively than control treatment. These results suggest that inoculation of seed bean by Rhizobium and mycorrhiza, can improve yield and resistance to drought stress by increasing of micronutrients concentration in seed bean plant.

Today, numerous studies are being done to replace chemicals with natural substances in order to reduce synthetic compounds in food. Garlic (Allium sativum L.) is an important bulb vegetable because of its high concentration of allicin, flavonoids and selenium accumulation. This research was carried out to investigate the effects of two levels of mycorrhiza (inoculation and non-inoculated), three levels of selenium (0 as control, 100 and 200 mg/l) on two ecotypes (Ramhormoz and Dezful) in terms of flavonoid, allicin, daily intake of selenium and yield of garlic. The experiment was performed as factorial in the form of completely randomized design. The highest values of flavonoid (27.618 mg/g) and Allicin (5.17 mg/g) were observed in Dezful ecotype and the highest daily selenium uptake (11.08 mg/kg) was observed in Ramhormez ecotype. Mycorrhizal inoculation was significantly and positively correlated with flavonoid and garlic yield at 1% level and with allicin at 5% level, but it was reversed with daily selenium uptake (EDI) at 5 % level. Also, application of selenium fertilizer showed a positive and significant correlation with increasing daily selenium uptake and flavonoids in garlic Bulbs at 1 and 5% levels, respectively. But, it has inverse correlation with allicin content at 1 % level. Dezful ecotype had a better performance in terms of garlic weight and garlic cloves weight and there was a significant difference between two ecotypes at 1 % level. Finally, due to different effects of the treatments, if the purpose is to produce garlic enriched with Se, flavonoid or allicin, a decrease in some nutritional values and a change in ecotype must be accepted.

In order to study the effect of phosphate and zinc bio-treatments on inorganic P fractionation in two cultivars of bean, an experiment was conducted as factorial in a completely randomized design. The research treatments consisted of two cultivars of bean (Talash and Sadri), four levels of P (P0: Control, P1: Use of TSP fertilizer on the basis of soil test, P2: 50 percentage of TSP + P biofertilizer, and P3: Use of P biofertilizer), and three levels of Zn (Zn0: Control, Zn1: 50 kg ha-1 ZnSO4, and Zn3: Use of biological Zn treatment). The results revealed that there were significant differences between the two cultivars on Ca2-P, Ca8-P, Ca10-P and total P. The minimum amount of inorganic P fractions was obtained from root zone soil of Sadri cultivar. The effect of P treatment was significant on inorganic P fractions, except Al-P, Fe-P and O-P. The amount of inorganic P forms showed an increase in P1 and a decrease in P2 and P3 treatments. The effect of Zn treatment was significant on inorganic P fractions, except Al-P and O-P. The minimum amount of inorganic P fractions was obtained from Zn2. Among the interaction effects, the triple interaction effect of cultivar × P × Zn was significant on Ca2-P and Ca10-P and the double interaction effect of P × Zn was significant on Ca2-P، Ca8-P, Ca10-P and TP. The maximum amounts of these forms were obtained from C1P1Zn0 and P1Zn0, and the minimum from C2P2Zn2, P2Zn2 and P2Zn3 treatments. Sadri cultivar and phosphate and Zn bio-treatments had higher efficiency in release and uptake of P inorganic forms.

In order to study the effect of biofertilizers, chemical fertilizers and water deficit stress on biological yield, lawson content and root colonization with mycorrhiza in henna5 an experiment was conducted at research greenhouse of bam university in 2014 and 2015 growing seasons . The factors were fertilizers ( without any fertilizer (F1), Humic acid(F2), application of Mycorrhizae and Vermicompost (F3), Vermicompost (F4) and Chemical fertilizer (F5) and Water deficit condition ( 100% W.R, 80% W.R and 60% W.R). The treatments were arranged as factorial in a randomized complete blocks design with fifteen treatments and three replications. Results showed that the highest weight of dry leaf, No of leaf and biological yield were obtained with application of mycorrhizae and vermicompost treatment (I1F3). With increasing stress severity, the highest weight of dry leaf (0.153g), No of leaf (171), Plant Height (120.33 cm) were obtained with in I2F3 and biological yield (5.21 g/plant) were obtained with application mycorrhizae and vermicompost treatment under 60% water requirement. The highest lawson content (69.6 mg) were obtained with in I3F3 treatment.The highest root clonization (82.2%) were obtained in I3F3 treatment. It seems that biofertilizers can consider as a replacement for chemical fertilizers in henna medicinal plant production.

Bean (Phaseolus vulgaris L.) is one of the Biological Nitrogen Fixation (BNF) in pulse crops. The simple and interaction effects between microorganisms, especially fungi, phosphate and Zinc (Zn) solubilizing microorganisms and Rhizobium bacteria can have very effective impacts on nutrient uptake, plant height, branch number, germination, plant growth, nodulation, BNF, colonization and yield in different plants.This study was done to evaluate the effect of Phosphate, Nitrogen (N), and Zn bio-fertilizers on growth morphological characteristics, nodulation, BNF and root colonization in two cultivars of bean. This experiment was carried out as a factorial in a randomized complete design (RCD) with three replications. The research treatments consisted of two cultivars of Pinto bean (Talash and Sadri), four levels of Phosphurus (P) (P0: Control, P1: Use of triple super phosphate (TSP) fertilizer on the basis of soil test, P2: 50 percentage of TSP recommendation and phosphate bio-fertilizer that consist of of inoculum of Funneliformiss mosseae, Rhizophagus intraradices and Clariodeoglumus etunicatum with Azotobacter bacteria , and P3: Use of P bio-fertilizer), three levels of N (N0: Control, N1: Use of urea, and N2: Use of biological Nitrogen that consist of Rhizobium legominosarium bv. phaseoli strain 133-136-111 inoculation), and Zn (Zn0: Control, Zn1: 50 kg ha-1ZnSO4, and Zn2: Use of biological Zn consist of Pseudomonas aeuroginosa strain MPFM and Pseudomonas fluorescens strain 187 inoculum). Grain inoculation was done in shadow and after drying, inoculated grains were immediately cultivated. The measured properties consist of morphological growth characteristics, nodulation, nodule number and root colonization. The results of experiment indicated that the effect of cultivar, P, N and Zn treatments was significant on studied parameters. The maximum of these parameters was obtained from Sadri cultivar, P2, P3, N2 and Zn2 bio treatments. The highest of studied parameters was obtained from P2Zn2, P2N2 and N2Zn2 treatments among the dual interaction effects. The concurrent inoculation of bean with mixture of mycorhizae + Azetobacter + R. leguminosarum + Pseudomonas significantly increased plant wet weight and nodule number. The triple interaction effect was significance on plant wet weight and nodule numbere only. The maximum amount of plant wet weight (45.3 gram per pot) and nodule number per shrub (28 nodules) were obtained from P2N2Zn1 and P3N2Zn2 treatments respectively. Despite the lack of significance difference interactions between triplicate treatments, the maximum amount of nodule grade, colonization percentage and BNF, 16.4, 44.9 perceent and 64.4 Kg ha-1 respectively were obtained from P3N2Zn2 treatment. Also, the highest amount of plant dry weight, the number of germinated shrub and emergency speed, 22.5 g pot-1, 4.3 shrub pot-1 and 0.54 shrub day-1 respectively were obtained from the combined treatments of P2N2Zn2 and P2N2Zn3. The maximum of studied parameters was obtained from P2, P3, N2 and Zn2 which represents the effective role of mycorrhizae fungi, phosphate and Zn solubilizing bacteria in increasing growth morphological properties, wet and dry weight, nodulation, colonization, BNF and reduction of P and N fertilizers. The studird parameters were increased with ombined using of double and triple of phosphate, nitrogen and zinc biotreatments. The combined use of phosphate, nitrogen and zinc, P2N2Zn2 treatment, is recommended to increase the morphological growth characteristics, nodulation and other studied parameters in this experiment

The effect of biofertilizer and chemical fertilizer on water use efficiency and physiological growth indices of henna as medicinal plant was studied at three water regimes in 2014-2015 grows. The research was carried out in an greenhouse experiment using a factorial based randomized complete bloch design with three replications. The factors were fertilizers ( without any fertilizer (𝐹1), humic acid(𝐹2), application of mycorrhizae and vermicompost (𝐹3), vermicompost (𝐹4) and chemical fertilizer (𝐹5) and water deficit condition ( 100% W.R, 80% W.R and 60% W.R). Results showed that the application of arbuscular mycorrhizae, humic acid and normal condition (no water deficit), increased Plant height, Stem diameter, Leaf dry weight, Leaf fresh weight, No.khur, Plant dry weight and No.sub stem.The above mentioned characteristics were decreased under water deficit condition, non application of mycorrhizae and non application of humic acid. Application of mycorrhizae and humic acid significantly increased water use efficiency. The intraction effects of water deficit and mycorrhizae, was statisticaly significant. The highest water use efficiency (89.5𝑔⁄𝑚3 ) ocuured under water deficit condition (60%W.R) and application mycorrhizae and vermicompost. The lowest amount of water use efficiency (37.42𝑔⁄𝑚3 ) observed without any fertilizer under normal condition ( no water deficit).

Phytoremediation is a biological method by which plants are used to remove environmental pollution caused by toxic elements such as the accumulation of cadmium, copper, lead, chromium, etc. from soil. The arbuscular mycorrhizal fungi (AMF) help the plant to increase uptake and translocation of low mobility nutrients and also micronutrients. In this research, arbuscular mycorrhizal inoculant was produced through the isolation of fungi from the roots of marigold and its propagation with clover and sorghum under greenhouse conditions. Moreover the symbiotic effect of arbuscular mycorrhizal fungi on the concentration and transfer of cadmium from the roots to the shoots in Marigold was studied under greenhouse conditions. The experiment was conducted using a factorial based on randomized complete books design with five levels of Cd (0,100, 200, 400, 800 mg kg-1 soil), using the soluble from of CdCl2.H2O and two level of mycorrihizal inoculation (non- inoculated and inoculated) with four replications. Greenhouse test results showed concentrations of cadmium in shoots ranges between 3.91 to 94.9 and in roots between 4.23 and 151 mg.kg-1, which causes toxicity in the plant, with more severe symptoms of toxicity in non-mycorrhizal plants. The results also showed inoculation of marigold with AMF caused increase of P translocation to shoot and accumulation of Cd in roots. Phytoremediation is a biological method by which plants are used to remove environmental pollution caused by toxic elements such as the accumulation of cadmium, copper, lead, chromium, etc. from soil. The arbuscular mycorrhizal fungi (AMF) help the plant to increase uptake and translocation of low mobility nutrients and also micronutrients. In this research, arbuscular mycorrhizal inoculant was produced through the isolation of fungi from the roots of marigold and its propagation with clover and sorghum under greenhouse conditions. Moreover the symbiotic effect of arbuscular mycorrhizal fungi on the concentration and transfer of cadmium from the roots to the shoots in Marigold was studied under greenhouse conditions. The experiment was conducted using a factorial based on randomized complete books design with five levels of Cd (0,100, 200, 400, 800 mg kg-1 soil), using the soluble from of CdCl2.H2O and two level of mycorrihizal inoculation (non- inoculated and inoculated) with four replications. Greenhouse test results showed concentrations of cadmium in shoots ranges between 3.91 to 94.9 and in roots between 4.23 and 151 mg.kg-1, which causes toxicity in the plant, with more severe symptoms of toxicity in non-mycorrhizal plants. The results also showed inoculation of marigold with AMF caused increase of P translocation to shoot and accumulation of Cd in roots.

Drought stress is one of the main problems in agricultural productions in arid and semiarid regions such as Iran. Lack of water influences on most of plant physiological processes such as photosynthes, cellular development and uptake and transmission of nutrients in plants. Some approaches such as selection of resistance cultivars to drought stress, and selection of dripped irrigation have been applied in order to increase the irrigation efficiency. In recent years, biological approaches such as mycorrhizal symbiosis have been used to alleviate the detrimental effects of drought stress. Mycorrhizal symbioses increase the absorption of nutrients, especially phosphorus, and reduce the adverse effects of environmental stresses. It can also improve the host plant growth and yield. The percentage of mycorrhizal dependency of host plants depends on different environmental factors (such as light intensity, temperature, soil conditions), as well as morphological and physiological characteristics of plants. 1n 2010, a greenhouse pot experiment was conducted at University of Agriculture and Natural Resources Ramin. The effect of mycorrhizal inoculation on root morphology of three leek genotypes and uptake of phosphorous, calcium and potassium in shoot and root were studied. The experiment was conducted in a completely randomized design consisting of a 3×3×2 factorial combination. Experimental factors included three levels of soil moisture 40, 60 and 80% of available water in the soil), two mycorrhizal status (with and without fungus Glomus intraradices) and three leek genotypes including: Shadegan (with low root branching, short and thin root length), Esfahan (with abundant root branching and long root length) and Porrum (with low root branching, short and thick root length). The treatments were replicated four times. The soil was autoclaved at 121°C and15 PSI for 15 minutes and gently packed into PVC pots, 200 mm long and 150 mm in diameter. Leek seeds were sterilized with sodium hypochlorite (NaOCl) solution (10%) for 20 min. Two hundred grams of inoculum (spore, hyphae, mycorrhizal clover of root fragments and soil) were placed in deep of plant root. Each pot received cm-3 nutrients solution, free of P weekly. Plants equally watered for one mounth then, drought stresses were applied. Leeks were harvested 12 weeks after planting. Sub-samples of roots were taken for determination of root length were cleared in 10% (w/v) KOH solution and then were stained with trypan blue and root colonization was studied using modified Phillips & Hayman. The colonized root length was determined by binocular and gridline intersect method of Tennant. Phosphorus concentrations were measured by the method of colorimetery with a spectrophotometer. Potassium and calcium concentrations were determined by flame photometer and titration with vercin (Ethylene diamine tetra acetic acid: EDTA), respectively. The statistical analysis was performed using MSTAT-C statistical software and means were compared by Duncan’s multiple range test at the significance level of P<0.05. The results showed that the total content of phosphorus, potassium and calcium was reduced with increasing drought stress. The concentrations of phosphorus, potassium and calcium in root and leaves of three genotypes were different depending on genotype, morphological root characteristics of leeks. At all drought stress levels, the concentration and total content of three elements were greater in mycorrhizal treatments compare to non- mycorrhizal treatments. At all drought stress levels, the total content of three elements in unit root length were greater in mycorrhizal treatments compare to non- mycorrhizal treatments because of Mycorrhizal colonization significantly increased leeks root length and root dry weight in all drought stress levels. The results also revealed that Shadegan genotype with weak root system had high dependency on Glomus intraradices that ultimately led to a higher uptake of nutrients. These findings supported the Baylis’s hypothesis even in drought stress condition Clearly, the results of this study indicated that mycorrhizal fungi inoculation improved the nutritional status of leeks and the negative effects of drought stress were reduced by increasing the absorption of phosphorus, potassium and calcium. leek genotypes response to mycorrhizal symbiosis under drought stress was related to root morphology. Shadegan genotype with low root branching and short and thin root length (weak root system) had stronger symbiotic relationship with mycorrhizal fungi compared to the other genotypes which has ultimately led to a higher uptake of nutrients (phosphorus, potassium, calcium) in mycorrhizal Shadegan leeks.

Plants are the most sources for cadmium enters to human food and this element in food regime may be a serious threat to human health. Thus, the effect of Azotobacter inoculum (Azotobacter chroococcum), mycorrhiza fungi (Glomus intraradaices), zinc sulphate and urea on cadmium Convertation and growth parameters of fenugreek medicinal plant (Trigonella foenum gracum L.) in cadmium polluted soils were evaluated. This experiment conducted in randomized complete block design with three replications at the research greenhouse of Shahrekord University. Eight fertilizer treatments included control (without fertilizer), urea, urea+ zinc sulfate, urea+ Azotobacter, urea+ mycorrhiza, urea + zinc sulfate + Azotobacter, urea + zinc sulfate + mycorrhiza, urea + zinc sulfate + mycorrhiza + Azotobacter. The results indicated that the highest of plant height, leaf fresh weight and shoot fresh weight of the aboveground of fenugreek were obtained in urea+ Azotobacter but it didn’t have a significant difference with that of the urea + zinc sulfate + mycorrhiza. Also, the lowest cadmium concentration (0.09 mg/kg) was produced in urea+ Azotobacter and urea + zinc sulfate + mycorrhiza (P≤0.05). Appliction two biofertilizers sources plus zinc sulfate in compared to chemical fertilizer treatments had appropriate vegetative growth and fairly cadmium concentration. In conclusion, Azotobacter application for fenugreek production in addition to increase yield and can be effective in preventing of cadmium transmission in the human food regime as well as.

Mycorrhizal status of pistachio seedling in Kerman province in regard to region and some soil properties and also symbiotic potential of Sorghum, clover and onion isolates with pistachio seedling were evaluated in this research. 72 samples of pistachio nurseries and 67 samples of Sorghum, clover and onion farms were gathered from Kerman province and mycorrhizal colonization percent of their roots were determined after clearing with KOH and staining with Tryphan blue. All soil samples were analyzed for EC and pH and then for soil texture of samples with different colonization percent. The result showed that all of samples had mycorrhizal symbiosis and the colonization percent was varied from minimum 33 to maximum 97 percent. The colonization percent of Kerman, Mahan and Bardsir samples was related mainly to 75-100% range, but 25-50% for Zarand samples. Based on regression test, there was a significant relation between soil texture and pH (α=5%) and also EC (α=5%) with colonization percent. The colonization percent were decreased significantly in soil salinity more than 4 dS/m. Inoculation of pistachio seedling with 20 mycorrhizal isolates of pistachio, sorghum, onion and clover plants via a greenhouse experiment showed that all isolates had colonization potential of pistachio roots after 60 days and colonization percent was varied from minimum 63 to maximum 88 percent. Inoculation of Pistachio seedlings with selected isolates had significant effect on most vegetative index of seedlings in compare to control treatment.

Under arid and semi-arid conditions, water deficiency may limit the use of nitrogen by plant and lead to a decline in crop production and water use efficiency. Therefore, in order to study the effect of drought stress and various fertilizers on different aspects of water use efficiency and biomass of isabgol, a factorial experiment was conducted in a randomized complete blocks design with four replications under microcosm conditions. Treatments included two levels of drought stress (non-stress and with stress) as the first factor, two levels of mycorrhizae (inoculated with G. intraradices and non-inoculated) as the second factor and three fertilizer types (separate application of Azotobacter chrococcum, application of 40 kg N/ha as urea fertilizer, and combined application of azotobacter plus 20 kg N/ha as urea) as the third factor. The results indicated that drought stress reduced the root and shoot biomass on average by 31% and 28%, respectively. But, mycorrhizae caused the increase of root and shoot biomass on average by 65% and 24.7 %, respectively. Water use efficiency increased for the aboveground biomass and total biomass production in drought stress plus mycorrhizae and 40 kg N/ha as urea. However, water use efficiency for seed and mucilage production was only affected by drought stress and mycorrhizae. In conclusion, results showed that water use efficiency for seed production was only increased by mycorrhizal inoculation under drought stress. However, water use efficiency for mucilage production not only increased in drought stress conditions, but also it increased more in mycorrhizal inoculation treatment under drought stress conditions.

Sesame (Sesamum indicum L.) is one of the most important oilseed crops in the world. Drought stress is one of the environmental factors limiting sesame production. The effects of water deficiency can be reduced by inoculation of plant roots with mycorrhiza fungi. In this experiment، the objective was to determine the effects of different levels of water application (60، 80 and 100% of soil moisture depletion) using surface drip irrigation method and inoculation of plants with mycorrhiza on WUEag of sesame. The experiment was arranged using a factorial design based on randomized complete block design with three replications. The field experiment was conducted at the Agricultural Research Farm of Isfahan University of Technology in 2011. Results showed that the effect of irrigation regime on WUEag of seed yield was not significant. However، the effect of mycorrhiza on WUEag of seed yield was significant. The highest WUEag (0. 74 kg/m3) was related to irrigation regime of 80% and mycorrhiza. The effect of irrigation regime and mycorrhiza on WUEag of oil yield were significant. The highest WUEag (0. 43 kg/m3) was related to irrigation regime of 100% and mycorrhiza inoculation.

Effect of drought stress and mycorrhizal symbiosis on growth and phosphorus (P) uptake by two sorghum cultivars different in root morphology was studied in a pot experiment. A factorial experiment with a randomized complete block design was performed. In this study, sorghum speed feed cultivar with high root proliferation and branching and sorghum KFS2 cultivar with low root proliferation and branching were colonized by Glomus intraradices. All plants were watered equally in the first three weeks to establish them. Drought stress was applied from the week four and the plants were watered when 40% (T1), 60% (T2) and 80% (T3) of the available water was consumed. Results showed that in both sorghum cultivars, plant dry weight decreased as drought stress was increased. However, dry weight of mycorrhizal plants was higher than that of non-mycorrhizal plants at all levels of drought stress. This increase for speed feed cultivar was greater than for KFS2 cultivar. Phosphorus concentration and total P uptake in shoot of both mycorrhizal plants were greater than those of non-mycorrhizal plants. P accumulation in both cultivars decreased by increasing drought stress due to the decline in plant biomass. However, P accumulation per unit length of colonized root increased by increasing drought stress. The greater percentage of root colonization and the increased total length of external hyphae per unit length of colonized root of KFS2 cultivar led to an increase in mycorrhizal growth response and improved P nutrition of KFS2 cultivar compared to those of speed feed cultivar.

Soil enzymes are the catalysts for important metabolic process functions including the decomposition of organic inputs and the detoxification of xenobiotics. The aim of this research was to determine the pattern of variation in the activities of dehydrogenase, urease, lipase and phosphatase enzymes, determining the number of hetrotrophic and degrading bacteria and measuring the soil respiration and yield plants during the bioremediation of oil contaminated soils. To this aim, the soil deliberately contaminated with crude oil at a 1 and 2 wt% rate and in four treatments including: plant multiflorum (T1), plant multiflorum with mycorrhiza inoculation (T2), plant multiflorum with oil degrading bacteria inoculation (T3), plant multiflorum with mycorrhiza and oil degrading bacteria inoculation (T4) was employed for bioremediation of oil contaminated soil. The above parameters were determined in five stages during bioremediation and ultimately for the yield of plants at the end of this period. The results showed that the activity of urease and hydrogenase anzymes were increased or decreased parallel to contaminant increase and decrease. In contrast, the activity of lipase anzyme was decreased with contaminant increase and increased with contaminant decrease. Therfore, it can be a good choice for monitoring of bioremediation of contaminated soils. The results showed that the number of degrading and hetrotrophic microorganisms were increased by increasing the amount of contamination and the number of degrading and heterotrophic bacteria were decreased parallel to contaminant decreasing especially in those samples treated with mycorrhiza inoculation. The plant yield and amount of degradation of oil compounds were highest in mycorrhiza plus degrading bacteria treatment.