فسفر

Introduction: 

Pre-cultivated plants are types of plants that, after harvesting the economic part, the plant residuals are returned to the soil to increase the organic matter and to improve the next plant’s productivity. The use of plant residues can improve biological health by reducing the chemical inputs used in agriculture. Due to the fact that in dry areas such as North Khuzestan, the role of pre-cultivated plants and the effect of their residues on growth and performance have been studied less, this research can be done in different humidity conditions before planting the main plant, and the effects of plant residues and their role on the qualitative characteristics and organic matter of the soil and the performance of plants such as corn are promising and help farmers and researchers.

 This experiment was conducted in two neighboring farms. In each year, two experiments were conducted in a randomized complete block design with four replications in two years and two locations. Before planting corn in two fields, cultivation treatments and mixing residues of four plant species, including rice, cowpea, dill, eggplant, and fallow, were used as experimental treatments. After harvesting the pre-cultivation plant, investigate the effect of plant residues on some soil characteristics over time. In order to determine the amount of moisture stored in the soil, in two stages before planting and in the middle of the corn growing season, the percentage of soil moisture by weight was calculated. To determine the organic carbon content in the soil before planting and during the mid-growth stage of the corn plant, organic carbon was oxidized using potassium dichromate in the presence of concentrated sulfuric acid, followed by titration with semi-normal ammonium ferrous sulfate in the presence of the ortho phenanthroline reagent (Walkley, 1934). Additionally, soil nitrogen was measured in the laboratory using the Kjeldahl method (Pag et al., 1982).

In both farms, the stability of the soil grains in the second year was higher than in the first year, which is due to the placement of organic materials of plant residues in the structure of the soil grains (Koocheki et al., 2020). Soil stability increased in both farms in the second year, with the difference that the soil stability increased by 64% in WET Planting and 50% in dry Planting. In the first and second years, respectively, there was a 35 and 29% increase in soil moisture compared to the control. The results of this research showed that the positive effect of plant residues in improving the organic matter of the soil after corn cultivation in wet Planting was much higher than that of dry Planting. The rice residues increased the soil organic matter by 51 and 47% in wet Planting and dry Planting. The results of the measurement data before corn cultivation showed a significant increase in the amount of nitrogen in the soil in the second year, and this increase was 55% higher in the field with the method of wet Planting cultivation. The results of the measured data after corn cultivation in the second year showed that the amount of nitrogen in the soil increased, and the intensity of the increase in the field using the wet Planting method was 44% more than the field using the dry method.

 The results of this experiment showed that the apparent specific weight of the soil in the second year, influenced by the use of plant residues, decreased over time. The preservation of plant residues has caused an increase in the amount of nitrogen in the soil, and in wet planting conditions, the intensity of its increase has been 44% more than in DRY Planting conditions. Also, by using plant residues of dill and beans, the amount of phosphorus in the soil increased.

This research was conducted in order to reduce or replace phosphate fertilizer with humic acid to investigate growth characteristics, grain yield and absorption of macro and micronutrients in safflower plants in order to reduce costs and increase the harvest. Cultivation of safflower of Goldasht variety with 9treatments were carried out a randomized complete block design. Morphological, vegetative growth and Yield traits and nutrients were measured in safflower leaves.The P50H100 treatment had the highest values in plant height, stem diameter, number of main branches, photosynthesis, chlorophyll index, stomatal conductance, capitol number, 100-grain weight, seed yield, biological yield, harvest index, oil percentage and oil yield. After P50H100treatment, H200andP25H150 treatments had better results in these indicators. P50H100treatment has the highest significant percentage of leaf nitrogen, P50H100and P50treatment has the highest significant percentage of leaf phosphorus, H200, P25H150and P50H100treatments have the highest significant amount of leaf potassium, H200treatment has the highest significant amount of leaf calcium, P25H150treatment has the highest significant amount of leaf magnesium, H200 treatment has the highest significant amount of iron and zinc in leaves, The H200 and P25H150 treatments had the highest significant amount of leaf manganese and the P25H150 treatment had the highest significant amount of leaf copper.This research showed that the most significant performance can be achieved with P50H100 treatment. After this treatment, H200 and P25H150 treatments had significant results and were appropriate. P100 and P50 treatments as well as H100 and P25H50 treatments did not have significant results compared to the superior treatments.

The aim of this experiment was to investigate the effect of biofertilizers such as phosphatebarvar-2 and seaweed extract in increasing growth traits, quality and quantity of coriander.

This study was carried out as a field experiment in a factorial based on completely randomized design with four replications. in the west of Iran, in 2016-2017. The first factor was consisted of seaweed extract in three levels (no extract, one percent and two percent) and the second factor was including phosphatebarvar-2 in two levels (inoculation and no inoculation).

The results showed that the interaction between phosphatebarvar-2 and seaweed extract had a significant effect on plant height, seed essential oil content, and phosphorus concentration in aerial parts. The highest essential oil content (0.448%) was obtained in the level of % 2 seaweed extract and inoculation with phosphatebarvar-2 fertilizer. The highest (4.962 kg/ha) essential oil yield was obtained in the treatment of inoculation with phosphatebarvar-2 and 1% seaweed extract. Also the highest (3.583%) amount of phosphorus in aerial parts was obtained in the treatment of inoculation by phosphatebarvar-2 and %2 seaweed extract.

The use of phosphate biofertilizer along with seaweed extract can improve the growth traits and quality of essential oil in coriander.

The present study was conducted to study the effect of different concentrations of Bacillus subtilis on morphophysiological traits of flowers and coriander in two saffron ecotypes in order to determine the appropriate concentration of bacteria.

A factorial experiment was conducted in a randomized complete block design with three replications in the research farm of Zanjan University. Experimental treatments: the first factor of five levels of different concentrations of bacteria(zero, 102, 105, 106, 108 ) and the second factor consisting of two ecotypes (Natanz, Zanjan) were considered.

Different concentrations of bacteria showed a significant effect on all morphophysiological traits measured. The main effect of ecotype also showed a significant effect on the traits at the level of one and five percent, except for the fresh weight traits of stigma, chlorophyll. Based on the results of analysis of variance, bilateral interaction (year × ecotype, ecotype × bacterium) and tripartite interaction (bacterium × year × ecotype) on flower yield and phosphorus content of saffron leaves were significant. Among the ecotypes, Natanz ecotype showed more than Zanjan ecotype in fresh and dry weight of pistachio and its diameter (16.29, 10.99 g and 19.71 mm, respectively).

Considering the effects of Bacillus subtilis on saffron ecotypes, it can be concluded that saffron ecotypes showed different reactions to biofertilizer due to their differences and different growth characteristics. According to the results, the greatest effect of Bacillus subtilis on morphophysiological traits was observed at a concentration of 108 cfu / ml.

The appropriate amount of nitrogen consumption and the positive effects of wheat straw mulch in medicinal plants are important to reduce the negative effects of drought stress and achieve high yield.

In this study, the growth and yield of black seed by combining nitrogen and wheat straw mulch at different irrigation intervals. This research was conducted as a split plot in a randomized complete block design. The first factor is different irrigation intervals in three levels including 25, 50 and 75% moisture drainage and the second factor in six levels includes 50 and 100 kg of urea per hectare in combination with mulch and consumption alone, mulch and control.

Increasing irrigation intervals decreased the amount of leaf elements. Also, an increase in ion leakage and decrease in the relative leaf water content were observed in 75% of moisture depletion. Application of wheat straw mulch was able to reduce ion leakage of black seed under drought stress conditions. Urea was effective in increasing nitrogen uptake and as a result increased the amount of photosynthetic pigments. At 25% moisture discharge, shoot dry weight per 100 kg of urea with wheat mulch and consumption alone showed an increase of 8.7% and 7.1%, respectively, compared to the control. The highest harvest index and yield components were observed in the use of mulch in combination with urea.

In total, the application of 100 kg of urea per hectare in combination with wheat straw mulch for planting black seed is recommended.

Catnip (Nepeta cataria L.) is one of the most important medicinal plants belonged to the Lamiaceae family that referred to considerable pharmacological, antimicrobial, antibacterial and pesticides activities of its essential oil in most of the literatures. The harmful effects of chemical fertilizers on the environment and human health and lack of soil organic matter in arid and semi-arid regions have resulted in an increase in the use of chemical fertilizers in these areas.Excessive use of these fertilizers has led to serious environmental pollutions during the last decades. With respect to the hazards of chemical fertilizers and the importance of medicinal plants, researchers have recently considered the application of biofertilizers and organic materials. According to literatures, the application of biofertilizers and organic fertilizers can be recommended as a proper alternation for the improvement of the quantity and quality of essential oil in Catnip.

This research was conducted as factorial layout based on a randomized complete block design (RCBD) with three replications at the research farm of the faculty of agriculture, Shahid Chamran University of Ahvaz, Iran. The first factor was included fertilizer treatments in five levels (Control (C), Azetobarvar-1 (Az), Phosphatbarvar-2 (Ph), Combination of Az and Ph (M), chemical fertilizer (Ch) included 150 kg.ha-1 granular urea (46% N) and 100 Kg.ha-1 triple superphosphate (46% p < sub>2O5)) and the second factor was comprised humic acid in two levels (0 and 20 Kg.ha-1). The studied traits in this research included soil microbial respiration and biomass carbon and soil organic matter contents, leaf nitrogen and phosphorus amounts, number and size of secretory trichomes, essential oil content, yield and composition. Leaf nitrogen and phosphorus concentrations were measured at the beginning of reproductive stage by Kjeldahl method and colorimetric method using a UV-2100-Unico spectrophotometer, respectively. Moreover, the soil microbial respiration and soil biomass carbon were determined by alkali absorption and the fumigated extraction, respectively, and soil organic matter was estimated by the modified Walkly and Blackʼs methods. Furthermore, the number and size of secretory trichomes was registered using Binocular microscope and essential oil was extracted by Clevenger-type apparatus and analyzed using GC and GC-MS at full bloom stage.

According to the results, the sole application of fertilizer treatment was positively affected on soil microbial respiration and biomass carbon, leaf nitrogen and phosphorus contents. The number and size of secretory trichomes, essential oil content and yield and essential oil composition were significantly influenced when fertilizer treatments and humic acid were applied simultaneously. The highest amount of soil microbial respiration as well as biomass carbon was detected in M and the lowest was observed in control. The application of Ch and humic acid was significantly improved the soil organic matter. However, no significant difference was found between these treatments and biofertilizers treatments. The lowest amount of soil organic matter was detected in control. The maximum content of leaf nitrogen element was observed in plant treated by Ch and minimum was registered in control. The highest level of leaf phosphorus element, the size of secretory trichomes and essential oil content were obtained when Ph was applied while there was no significant difference between this biofertilizer and Ch and M. The maximum number of secretory trichomes and essential oil yield was detected in plant treated with M. The most amounts of isomers 1 and 2 of nepetalactone and caryophyllene oxide was observed in Az, Ch and control, respectively, and least was in control, Ph and Ch, respectively. The highest level of trans- caryophyllene and (E,E)–Farnesene  was obtained in the oil of plant treated with M+ and Ch+ and lowest detected in Az+ and Ch-.

According to the results, to improve the quantity and quality of Catnip essential oil, the application of biological fertilizers instead of chemical fertilizers is recommended and further research is also required to investigate both the effects of applying humic acid alone and in combination with biofertilizers on some traits.

Intercropping systems are one of the sustainable agricultural systems that defined as growing two or more plants simultaneously, lead to the use of more resources efficiently of nutrient water and land, improving plant productionIntercropping, as a new green revolution, is a sustainable strategy for the development of food production due to the lesser reliance of chemical fertilizer inputs compared with monocultures. In intercropping systems, plant nutrient uptake could improve the physical, chemical, and biological soil properties and higher nutrient mobilization in the rhizosphere. Arbuscular mycorrhizal fungi (AM) are preferred biofertilizers over other myriads of microorganisms that inhabit the interface between plant and soil. They are ubiquitous soil inhabitants and form the largest group which is predominantly associated with crops. Arbuscular mycorrhizal fungi can considerably improve plant growth, nutrients uptake, and transport, especially phosphorus, water status, and chlorophyll content. Previously studies demonstrated that higher productivity with AM fungi's application was attributed to higher nutrients availability such as P, K, Ca, Mg, etc. Thus, an experiment was conducted to evaluate the effects of barley's different intercropping patterns with grass pea and symbiosis with AM fungus on the forage yield and nutrients absorption, including N, P, K, Fe, Zn, Mn, Ca, and Mg.

In order to investigate the forage nutrients content and in intercropping of barley (Hordeum vulgare L.) with grass pea (Lathyrus sativus L.) under application of arbuscular mycorrhizal fungi, a field experiment was performed as a factorial layout based on randomized complete block design (RCBD) with ten treatments and three replications at the faculty of Agriculture, University of Maragheh, Iran, during 2017. The first factor included different planting patterns (monoculture of barley, monoculture of grass pea, 75% grass pea+ 25% barley, 50% grass pea+ 50% barley, 25% grass pea+ 75% barley), and the second factor was inoculated and non-inoculated with Glomus intraradices fungus. All data were statistically analyzed using analysis of variance (ANOVA) using MSTAT-C statistical software. The Duncan's multiple range test was used to compare means at a 5% probability level.

This study demonstrated that the macro and micronutrient content were significantly affected by different cropping patterns with the application of AM fungi. The greatest barley forage yield belonged to inoculated barley monoculture. Furthermore, the results demonstrated that the inoculated barley forage yield in monoculture was 47.48% more than the non-inoculated. The highest grass pea forage yield was achieved in monocultures and followed by a ratio of 75% grass pea+ 25% barley symbiosis with mycorrhizae fungus. Also, the highest nutrient content was achieved in grass pea monoculture with the application of AM fungi. Between different intercropping patterns, the highest nutrients content was obtained in the 75% grass pea+ 25% barley with the application of AM fungus. The higher nutrients uptake was attributed to increasing the absorption surface and improving nutrients availability with the application of AM fungi. Also, Varma et al. (2018) reported that the application of AM fungi in intercropping systems increased the transfer of the nutrients, especially nitrogen, to component plants that resulted in higher nutrients uptake in plants. These authors also reported that the higher nitrogen content with the application of AM fungi attributed to the higher activity of nitrate reductase and dikinase glucan enzymes that resulted in higher nitrogen availability for plants.

According to the results of this research, intercropping treatments of barley/grass pea with Glomus intraradices considerably influenced by the absorption of nutrients and forage yield. The content of concentration nitrogen, phosphorus, potassium iron, zinc, manganese, magnesium, and calcium were highest at all intercropping patterns coincided with the application of mycorrhiza fungi than the non-inoculated monoculture of barley. Also, between different intercropping patterns, the highest nutrients content was obtained in 75% grass pea+ 25% barley pattern accompanied by application of mycorrhiza that suggested as a better pattern to achievement high-quality forage for farmers. Therefore, intercropping of barley/grass pea with the application of mycorrhizae can improve forage quality of barley and grass pea from the point of view concentration of nutrients and were influential in production forage with high quantity.

Khuzestan province is one of the important regions for sugarcane production with more than 100,000 hectares under sugarcane cultivation. Sugarcane monoculture is common all over the world and continuing this system gradually reduces the yield and soil fertility. Monoculture systems, by creating unfavorable biological and physicochemical conditions in the soil, reduce the amount of organic carbon and nutrients in the soil. However, in an agricultural system, suitable agronomic selection, proper management of agronomic operation specially plowing, proper use of chemical and organic fertilizers, preservation of vegetation and proper management of plant residue have a decisive role in the amount and dynamics of mineral and organic elements. Various studies showed that rotation, particularly with green fertilizers, have led to breaking the pathogenic cycle and improving the physical and chemical properties of soil, including soil organic matter.

In order to evaluate the changes in the amount of carbon and organic matter, nitrogen, phosphorus and potassium residue in soil under different crop rotation conditions, this study was performed in a randomized complete block design with three replications during 2014-2016 for three consecutive years in the field of Amirkabir sugarcane Agro-Industry. The experiment treatments consisted of ten alternatives: 1) wheat-clover-sugarcane; 2) rapeseed-clover-sugarcane; 3) barley-clover-sugarcane; 4) sorghum-clover-sugarcane; 5) clover-vetch-sugarcane; 6) clover- clover- sugarcane, 7) clover- clover (third aftermath)- sugarcane, 8) clover- soybean- sugarcane, 9) clover- corn - sugarcane, and 10) fallow- fallow- sugarcane. The results showed that there was no significant difference between the changes in phosphorus in three years of experiment at both soil depths, but the amount of potassium and organic carbon was decreased during the third year of experiment. Analysis of variance showed that the amount of P, N and organic carbon content were significantly different in both soil depths under crop rotations. Also, under crop rotation, potassium and C/N ratio were different in 0-30 cm and 30-60 cm soil depths, respectively. The highest amount of nitrogen, phosphorus and carbon to nitrogen ratio were observed in clover-clover- sugarcane, fallow-fallow-sugarcane and sorghum-clover-sugarcane.

The results showed that the changes in the phosphorus content during three years of implementation of the project based on variance analysis even at 0-30 C depth, or at a depth of 60 to 30 cm, did not have a significant difference and the amount of phosphorus was remained almost constant.NitrogenThe results showed that the process of nitrogen changes during three years of experiment was based on analysis of variance at 0-30 and 30-60 cm of soil depths has significant difference and the amount of nitrogen has changed.Organic matter, Organic carbon and C:NOrganic matters of the soil is as the most effective factor in the sustainability of pasture and forest ecosystems, soil protection layer against erosion, temperature regulator, habitat and food supply for soil organisms and the main place of mineral nutrition.Potassium Potassium plays an important role in soil fertility. The statistical analysis of soil potassium showed that the amount of potassium had significant differences in two studied soil layers during three years of project implementation.Sugarcane yieldThe results showed that the highest yields were related to the crop rotation of clover-mung bean-sugarcane and clover- clover (third aftermath)-sugarcane rotations with 124 and 121 t.ha-1, respectively. Also, the lowest yield was related to the varieties of fallow-fallow-sugarcane and clover-corn-Sugarcane, with 95 and 98 t.ha-1, respectively.

The results showed that phosphorus in the soil did not change significantly during the experiment, but the amount of potassium, organic carbon and soil organic matter during the first and the second years remained constant but it decreased during the third year. Also, the implementation of different crop rotation affected soil nutrient elements. The highest amount of nitrogen, phosphorus and carbon to nitrogen ratios were observed in clover-clover- sugarcane, fallow-fallow-sugarcane and sorghum-clover-sugarcane. The impact of crop rotation on sugarcane yield also showed that cultivating legumes of clover and mung bean before sugarcane has a great influence on sugarcane yield.

Use of chemical fertilizers is an essential component of modern farming and about 50% of the world’s crop production can be attributed to fertilizer use. Sustainable production of crops cannot be maintained by using only chemical fertilizers and similarly it is not possible to obtain higher crop yield using organic manure alone. A balanced fertilization is needed to obtain optimum potential yield. The continuous imbalanced use of chemical fertilizers is creating complexity in our soils and the soil health is deteriorating. Application of municipal solid waste compost (MSW) is commonly applied to the soils to improve their physical, chemical and biological properties. Soil amendment with MSW is very useful for agricultural crop production. Wheat (Triticum aestivum L.) is the world’s leading cereal crop both in area and production and about two-third of people of our planet live on it. It plays an important role in diet of human being as well as in industrial uses. The present study was conducted to better understand the effect of MSW and chemical fertilizers on quantitative and qualitative yield of irrigated wheat in kermanshah condition.

In order to study the effects of municipal solid waste compost (MSW) and chemical fertilizers on quantitative and qualitative yield of Bahar wheat cultivar, a field experiment was conducted with chemical fertilizers at four levels (0, conventional farmers, based on soil test and 25% lower than the soil test), and MSW at four levels (0, 10, 20 and 30 ton.ha-1) in a factorial experiment based on randomized complete blocks design (RCBD) with three replications in Kermanshah region during 2014-2015.Prior to sowing, MSW and chemical fertilizers was applied and mixed thoroughly with the soil. Before planting, combined soil sample from a depth of 0–30 cm was collected to determine some soil chemical properties.Plots were designed with 4 ×4 m, 1 m apart from each other., 1.5 m alley was kept between blocks. Wheat seeds (var. Bahar) were planted 2.5 cm apart from each other. During the growth and development stages, plots were irrigated based on the crop water requirement. Irrigation method was sprinkler. Grain yield were determined after the harvest and seed samples (harvesting stage) were taken and rinsed with distilled water, oven dried at 70°C, ground, digested and analyzed for determining the N, P, K, Fe, Zn, Mn, Cu, Pb and Cd concentration. Analysis of variance was performed using MSTAT-C and mean comparisons done by Duncan’s multiple range test (P ≤ 0.05).

Results showed that MWS and chemical fertilizers and their interaction effects had significant effects on experimental traits. Using of MWS with chemical fertilizers led to increase plant height, 1000 grain weight, seed number per spike, grain yield, protein percent and nutrients concentration in grain. The results of means comparison showed that the highest grain yield (5900 kg.ha-1), 1000 seed weight (39 g), grain number per spike (71), protein percent (12.6) and nutrients concentration in grain was obtained under the co-application of 10 ton.ha-1 MWS and chemical fertilizers (25 percent less than the soil test) and the lowest amounts of experimental traits were shown in control treatment. Our results are in agreement with some experiments which use of compost and chemical fertilizers on irrigated wheat.

The result of this experiment revealed that using of compost and chemical fertilizers in integrated form instead of individual application has a beneficial effect on improving the quantitative and qualitative yield of irrigated wheat. Based on the obtained results, co-application of 10 ton.ha-1 compost and chemical fertilizers (25 percent less than the soil test) at kermanshah condition can be recommended.

Plants under natural and agronomic conditions are constantly exposed to different stresses. In this regard, drought stress is the most important limiting factor to crop yields in many parts of the world and Iran, particularly, if the water stress occurs during the flowering stage, it will affect the crop production. Drought stress disrupts the nutritional balance of plant. Proper nutrition under stress conditions can partly help plant against various stresses. Plants growth under stress can be improved by micro nutrients foliar application. Boron is important in the plant growth and its deficiency is one of the major constraints to crop production. Zinc plays a key role in the various biochemical processes of plant cells. Its deficiency will be apparent in various forms such as growth retardation, yield and concentration of the element in different parts of a plant such as seeds. The aim of this study was to investigate the effect of boron and zinc foliar application on yield and some agronomic and morphological traits of spring safflower (cv. Mahalli-e Esfahan) under late-season water deficit in Ardabil province.

The field experiment was conducted at the Research Farm of the University of Mohaghegh Ardabili, Ardabil, Iran during 2013-14 and 2014-15 growing seasons. The experimental design was a split factorial in a randomized complete block with three replications. Three irrigation treatments (S1: full irrigation until the end of season (control), S2: irrigation with holding from flowering and S3: irrigation with holding from seed filling) were randomized to the main plots and the sub-plot included factorial combination of B (B1: 0, B2: 350, B3: 700 ppm) and Zn (Zn1: 0, Zn2: 1000, Zn3: 2000 ppm). B was added as boric acid (H3BO3) and Zn as zinc sulfate (ZnSO4). The treatments were applied during flowering (when 50% of the plants were at anthesis) as foliar applications. All other agricultural practices (weeds control and irrigation), were performed when they were required and as recommended for safflower production. The measured traits included seed coat percentage, seed kernel percentage, seed coat to kernel ratio, leaf number, capitol diameter, seed length, seed width and seed yield. Analyses of variance and comparison of means at P≤0.05 were carried out, using SAS 9.1 software. The means were compared using LSD test.

Combined analysis of variance of the data showed that year, irrigation and year × irrigation interaction were significant for all the traits. Also, all traits (except leaf number) were significantly affected by B, Zn and B × Zn interaction. Seed coat percentage, seed kernel percentage and seed coat to kernel ratio were significantly affected by year×B, year × Zn and year×B×Zn interactions. Seed length and seed yield were significantly affected by year×irrigation×B and year×B×Zn interactions, respectively. During the first year, all the investigated traits influenced from deterrent impacts of drought stress. Water deficit stress at the flowering and seed filling stages significantly decreased seed yield compared to full irrigation (15.62% at flowering and 12.77% at seed filling). During the second year, there was no significant difference among all the treatments due to heavy rainfall. The results showed that B and Zn foliar application had a positive and significant effect on seed coat percentage, seed kernel percentage, seed coat to kernel ratio, capitol diameter, seed length, seed width and seed yield. The treatments of B3Zn2 and B2Zn2 had the greatest increase of these parameters in comparison with B1Zn1 (control) in both years. Boron and zinc foliar application significantly improved most traits under water stress levels.Acknowledgments We would like to thank from the Faculty of Agriculture, Mohaghegh Ardabili University, for supporting this study.

In this study, soil fungal isolated from Helianthemum ledifolium root and around of desert truffles were identified from ten different regions in Gonbad Kavoos in Golestan province. Desert truffles samples, were identified by morphological and molecular methods. Twenty one species of the fungal belonging to thirteen genera were isolated. Most of the fungal isolates were recovered from soil around desert truffle T. claveryi ascocarps and H. ledifolium rhizosphere respectively. Aspergillus and Fusarium were the predominant species which were isolated from soil around desert truffles. Acremonium, Paecilomyces, Polyschema and Ulocladium species were isolated with least abundance for the first time from desert area of Gonbad. Also Polyschema sclerotigenum was recorded for the first time in Iran. The results of the soil analysis collected from different regions showed that there was little difference in the structure and amount of lime in the soil. Most of the collected desert soil was alkaline, non-saline, with low organic matter, low phosphorus, sand and high lime.Amount of phosphorous was not significantly different in various desert soil samples.

In order to study the effect of irrigation levels (irrigation after 70, 110 and 150 mm evaporation from pan), phosphate fertilizer levels (control, 25 and 50% recommended phosphorus fertilizer) and mycorrhizal fertilizer (no application, G. mossae, G. intraradices, G. hoci and application of a mixture of mycorrhizal species) on peppermint,anSplit plot experimentlayout based on randomized complete block design with three replications at Azad University of Tabriz during two years of 2017 and 2018. In this study, Dehydration of soil caused a significant reduction in growth, yield and physiological functions, while the application of a mixture of three mycorrhizal species, the total dry weight increased by 36.9%. In the first year, the use of chemical phosphorus fertilizer and mixture of mycorrhizal species increased the total dry weight and essential oil yield.In the second harvest, in the second year, the application of 50% of the recommended dose of phosphorus fertilizer and the application of a mixture of studied species increased the yield of 18.37 and 38.19 percent in essential oil yield. The application of phosphate and mycorrhizal fertilizers in irrigation treatments improved the chlorophyll index and the content of chlorophyll a and b peppermint. Application of a mixture of mycorrhizal species and reducing the amount of phosphorus fertilizer to 50% of the recommended dose can improve the growth, essential oil yield and some physiological characteristics of the plant in both conditions of irrigation and dehydration.

Poor soil fertility is one of the main problems that limit the successful agricultural production and the global economy. Therefore, it is necessary to regulate or correct poor soil fertility, by providing vital nutrients for optimum plant growth. Improving soil fertility through fertilizer can increase crop production per unit area. But excess use of chemical fertilizers, in addition to disrupting the biological balance, nutrition and physical properties of soils, causes to environmental hazards, pollution of surface and ground water resources. Biofertilizers can reinforcement soil fertility  and provide the required plant nutrition with respect of sustainable agriculture. These organisms not only stimulate the growth of plants by helping the specific elements, but also reduce disease, improve soil structure and consequently increase the quantity and quality of their product. Pseudomonas, Bacillus, Azospirillum, Azotobater and Rhizobium are the most important plant growth promoting rhizobacteria (PGPR). Several mechanisms have been suggested by which PGPR can promote plant growth, including auxins, enhancing stress resistance, asymbiotic N2 fixation, solubilization of inorganic phosphate, mineralization of organic phosphate or other nutrients and inhibiting the growth of pathogenic microorganisms.

In order to determine the best time of application of Pseudomonas ssp. strains (sp.S10-1 + sp.S19-1 , sp.S14-3 + sp.S19-1 , sp.S19-1+ sp.S21-1) as a plant growth promoting rhizobacteria on growth and nutritional characteristics of tomato (Lycopersicon esculentum Mill cv. Super Chief) an experiment based on randomized complete block designed with 8 treatments and 3 replications in field of research station Khalatpoushan Faculty of Agriculture, Tabriz University. Treatments used in this experiment included : 1-  Inoculation with Pseudomonas bacteria sp.S10-1 + Pseudomonas sp.S19-1 during seed planting in the nursery, 2- Inoculation with Pseudomonas bacteria sp.S14-3+Pseudomonas sp.S19-1  during seed planting  in the nursery, 3- Inoculation with Pseudomonas bacteria sp.S19-1+Pseudomonas sp.S21-1  during seed planting  in the nursery, 4- Inoculation with Pseudomonas bacteria sp.S10-1 + Pseudomonas sp.S19-1 during planting seedlings in the main land 5- Inoculation with Pseudomonas bacteria sp.S14-3+Pseudomonas sp.S19-1 during planting seedlings in the main land 6- Inoculation with Pseudomonas bacteria sp.S19-1+Pseudomonas sp.S21-1 during planting seedlings in the main land 7- control treatment without bacteria inoculation 8- fertilizer treatment (according to the soil test).  

The result showed that the effects of the timing of bacteria inoculation on tomato characters such as yield, fruit size and the percentage of marketable fruit, percentage of K, vitamin C is meaningful and the highest amount of yield, fruit size, the percentage of marketable fruit, percentage of K in fruit was in T2 treatment concluded (4130.9 g m-2 and 60.22 and 31.18 and 4.7 mg g-1) respectively and the highest amounts of the vitamin C in T3 treatment as 7.02 mg 100g-1 fruit weight. However the other measured traits, including percentage of percentage of N, P in plant leaves, length and diameter of fruit and percentage of the dry matter did not affected by the timing of bacteria inoculation. According to the result  timing of bacteria inoculation has a significant effect on  yield and quality improvement of tomato.

The results of this research showed that tomato seed inoculation with the Pseudomonas ssp. strains including (sp.S10-1 + sp.S19-1 , sp.S14-3 + sp.S19-1 , sp.S19-1+ sp.S21-1)  compared with soil inoculation  of  Pseudomonas ssp. strains significantly increased yield, vitamin C, potassium content of fruit and percentage of marketable fruit. However, the increase in the amount of potassium, phosphorous and nitrogen concentration in plant leaves was not significant. In addition to increasing the yield and quality of tomato, tomato seed inoculation with the Pseudomonas ssp. strains compared with soil inoculation with the strains Pseudomonas is considered a better method.

Phosphate solubilizing and nitrogen-fixing bacteria and mycorrhizal fungi play an important role in the supply of nutrients in plants. To evaluate the efficacy of plant growth promoting bacteria and arbuscular mycorrhizal fungi (AMF) on physiology traits of Tareh Irani a field experiment in a randomized complete block design with 10 treatments and 3 replications was conducted. In this experiment tareh Irani seeds, (Tabriz landrace) inoculated with bacteria 1) P. putida P13, Pantoea agglomerans P5 (the biofertilizer Barvar2), 2) Azotobacter sp. (in biofertilizer Nitroxin), 3) bacteria Azospirillum sp. AC46 I, Azospirillum sp. AC49VП, 4) bacteria P. fluorescens Chao, P. putida Tabriz 5) two species of AMF, Glomus versiforme, G. intraradices. Results indicated that the colonization percent, percentage of chlorophyll, vitamin C content, total poly phenoles, phosphorus and nitrogen (p ≤ 0.01) and pyruvic acid, soluble proteins, (p ≤ 0.05) were affected by biofertilizers. Combination of mycorrhizal fungi and nitrogen-fixing bacteria (Azotobacter sp.) was better than other biofertilizer treatments in aspect of improving of quantity and quality traits in Tareh Irani. So that the bacteria can increase the efficiency of mycorrhizal fungi treatments were combined because of better nutrition and more food is especially phosphorus and nitrogen.

One of the phenomena that lead to the deterioration of surface water quality specially reservoirs is eutrophication caused by nutrients increasing like nitrates and phosphates in water resources. In this study, nitrate, phosphate and other factors such as temperature, turbidity, biological oxygen demand were measured monthly in six stations during the years 1393-1394 and the status of eutrophication in Kani-Brazan wetland and its influencing factors were investigated. The concentration of phosphorus and nitrogen of the wetland water were classified to the fourth trophic level of Oligotroph, Mezotroph, Eutroph and Hypertroph, and by comparison with international standards of Nuremberg, wetland eutrophication status was determined. Results showed that the average annual value of the water temperature was 17.4°C, pH=8.02, turbidity=16.03 and BOD was 5.8 (max=8.2 in spring and min=6.9 in summer). The values of all forms of nitrogen varied between 0.4 to 0.8 mg/lit indicating the trophic health of the wetland. The amont of phosphorus in wetland was upper than the limit of 0.03 mg/lit indicated that continue of this process could have consequences for the wetland ecosystem and affects attractive aquatic environment greatly.

Mycorrhiza can be used as an efficient tool to increase nutrients productivity to achieve sustainable agriculture. This experiment conducted to investigate the interaction of phosphorus and mycorrhiza on growth characteristics and production of mini-tuber and nutrients uptake of seedlings under greenhouse condition. The experiment was arranged as factorial in completely randomized design with three replications in agricultural and natural resource research center of Hamadan in 2015. First factor included of inoculation with mycorrhizal fungi, Rhizophagus irregularis, (inoculated and non-inoculated) and the second factor was the amount of phosphorus (0.965, 0.482, 0.241 and 0.12 mM phosphorus according to H2PO-4 in Hoagland solution (100, 75, 50 and 25 percent of Hoagland complete phosphorus concentration (HCPC), respectively). The results showed that the maximum values of seedling establishment (98.3%), number of leaves (13.3), the number of mini-tubers (8.9), the total weight of mini-tubers per plant (53.2 g), shoot height (54.6 cm), phosphorus in mini-tubers dry matter (%0.38) and leaves (%0.363) observed in phosphorus 75% HCPC and inoculation treatment and no significant differences was seen relative to phosphorus 100% HCPC and inoculation treatment. Inoculation with mycorrhizal fungi significantly increased the mini-tubers and leaves nitrogen, dry matter and weight of mini-tuber relative to non-inoculated treatments. Generally, respect to that there was no significant difference between 75% and 100% phosphorus in inoculation treatment, the applying of mycorrhizal fungi can reduce agricultural inputs consumption without any significant decrease in yield.

The effects of grafting and vermicompost on yield and quality of tomato were studied by a factorial experiment based on completely randomized design with 4 replication in research greenhouse of Mohaghegh Ardabili University at 2013. Rootstocks (none-grafting, grafting on ‘Yedi’ and ‘KingKong’) and five volume of vermicompost (0, 15, 30, 45 and 60%) in substrate were considered as experimental factors. For this purpose, traits such as number of flower and truss, growth duration of fruits, number of fruits, fruits mean weight, fruit yield, TSS and TA of fruits, phosphorous and potassium content of aerial parts of plants were measured. Results showed that flowering in grafted plants significantly retarded in comparison to non-grafted plants. Addition of vermicompost into substrate significantly increased number of flowers, panicles and fruits per each plant, TSS and TA but mean weight and size of fruits was decreased. Application of vermicompost improved phosphorous and potassium content in aerial parts of plants. Interaction between grafting and vermicompost was significant on yield and growth duration of plants. In general, we can say that grafting on ‘KingKong’ and ‘Yedi’ rootstocks and application of vermicompost can improve yield and quality of tomato plants.

IntroductionSalvia leriifolia Benth. (Nowruzak) is a herbal plant from Lamiaceae family, which is exclusively native of Iran and Afghanistan. Existence of this species is reported in Khorasan and Semnan provinces of Iran. As extreme pressure of grazing and harvesting the nature has put this species at risk of extinction, and on the other hand this plant has numerous herbal values and its cultivation can produce the market needs as well as preserve it from extinction. Therefore its domestication is now a priority and optimized nutrition for cultivated plants is one of the important factors in quality and quantity enhancement of the product. The aim of this research was to study the effect of three main nutrient: nitrogen, Phosphor, and potassium (NPK) on growth of Nowruzak and measure its morphological reactions to these elements in the poor soil of its habitat.
Materials and methodsTo study the effect of different nutritious elements on quantitative properties of Nowruzak, a factorial experiment was conducted as randomized complete block design with three replications in the Research Field of Islamic Azad University of Gonabad in March 2014. In this experiment, nitrogen fertilizers in three levels (N1= control, N2=100 kg.ha-1 and N3=200 kg.ha-1 based on net nitrogen), Phosphor in three levels (0, 50 and100 kg.ha-1 based on P2O5) and potassium fertilizers in two levels (0- and 50 kg.ha-1 based on K2O) were applied of farm to specify the effect of each elements and their reactions on quantitative growth properties of Nowruzak.
Results and discussionResults show that the effect of different levels of nitrogen fertilizer on plant height, harvest index, cover crown diameter and number of fertile branches are significant at 1% level. In a way that levels of nitrogen fertilizer are placed in first, second and third group of Duncan multiple range test, respectively. The highest plant height with average of 41.97 cm is assigned to 200 kg net nitrogen . Moreover, application of 200 kg.ha-1 of net nitrogen led to highest cover crown diameter with average of 92.07 cm and led to increase the number of fertile branches with average of 31.75 per plant. The same trend as nitrogen observed for different levels of phosphorous. Application of 100 kg Phosphor resulted highest number of leaves with average of 140 leaf/plant. The highest plant height is also assigned to the group of 100 kg Phosphor fertilizer with average of 41.97 cm. Moreover application of this amount of fertilizer led to highest cover crown diameter (94.06 cm) which also resulted in increase of the number of fertile branches (average: 33.21 branches per plant).
The results of analysis of variance regarding potassium fertilizer show that its application has significant effect on all the measured parameters at 1% level. Application of 50 kg of potassium fertilizer, in comparison with no application, results in increase of the number of leaves (average: 122 per plant), plant height (average: 37.06 cm), cover crown diameter (average 78.13 cm) and the number of fertile branches (average 26.55). The obtained results show that Bajestan ecotype of Nowruzak showed an acceptable adaptability to fertilizer application in Gonabad city which indicated the poorness of the soil.
ConclusionAs chemical fertilizers are among the main factors of soil fertilization, Nowruzak medicinal plant showed a normal response to highest amount of applied fertilizers, therefore it is justified to apply chemical fertilizers in cultivation of this plant in the field to increase the plant size which is the economically valuable trait

Corn is one of the important forage plants for livestock. Yield improvement and forage quality are essential issues in forage production. Bio-fertilizers and soil amendments have great potential as suitable alternatives for chemical fertilizers, but are less known in this area. The combined application of zeolite and mycorrhiza can improve conditions for plant growth while preventing loss of nutrients from the rhizosphere. The researchers reported that the combined use of zeolites (5 percent of soil weight) and mycorrhizae reduced leaching of nitrogen and phosphorus (Molaee et al., 2010). This experiment was performed to study the influence on corn growth of zeolite as a soil mineral amendment and mycorrhiza, at different levels of phosphorus chemical fertilizer and concentration of grain elements.
The experiment was performed as factorial study based on the complete randomized block design with three replications at the Faculty of Agriculture of Shahrood University located in Bastam in 2013. The experiment factors included zeolites, at two levels, 0 and 9 tons per hectare and mycorrhiza species (Rhizophagus irregularis), at two levels, Inoculation and without inoculation and triple superphosphate chemical fertilizer at three levels, 0, 50 and 100 kg per hectare which were applied to corn single cross 704. Determination of plant root colonization was carried out based on the Giovannetti and Mosse method (Giovannetti and Mosse, 1980). Measurement of the amount of chlorophyll was carried out at the flowering stage (tassel ratings) using a chlorophyll meter (SPAD 502). For measuring the nutrients of grain digestion, the perchloric acid and nitric acid method was used at a ratio of 1 to 6 (Hanson, 1950). Phosphorus was measured with a spectrophotometer (Hanson, 1950), potassium with flame photometer (Ghazzanshahi, 1997) and calcium grain using titration (Ghazzanshahi, 1997).
The results showed mycorrhizal fungi inoculation with root corn resulted in an increased percentage of colonization of about 25%. The application of phosphorus fertilizer (p≤ 0.01), zeolite (p The results showed application of mycorrhiza with soil amendments (zeolite) can be an alternative to some parts of phosphate chemical fertilizers in sustainable agricultural systems for the cultivation of maize and can improve the quality of the grain. Since the plant variety was a forage plant, and given the importance of forage quality in animal nutrition, the application of biological fertilizers and soil amendments can prevent the excessive consumption of chemical inputs while achieving a favourable biological yield and improving forage quality on farms.